EC 3.5.2.2: dihydropyrimidinase
HydrolasesOpen in Browse
CatLog read 50 records for EC 3.5.2.2 from 4 papers, for 6 organisms. Names in these papers: D-hydantoinase, dihydropyrimidinase (SmelDhp), D-hydantoinase (D-PfHYD), D-hydantoinase AcDHTase and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 51 records for EC 3.5.2.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.2.2 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Enzymatic activity assay of D-hydantoinase by isothermal titration calorimetry. Determination of the thermodynamic activation parameters for the hydrolysis of several substrates | Agrobacterium tumefaciens | 22 |
| Structure of dihydropyrimidinase from Sinorhizobium meliloti CECT4114: new features in an amidohydrolase family member | Sinorhizobium meliloti | 16 |
| Characterization and Soluble Expression of D-Hydantoinase from Pseudomonas fluorescens for the Synthesis of D-Amino Acids | Pseudomonas fluorescens | 6 |
| Thermotolerant D-Hydantoinases for Efficient and Stereoselective Intermediate Synthesis of (R)-3-Isobutylglutaric Acid Monoamide, a Key Intermediate in Pregabalin Production | Anoxybacillus caldiproteolyticus, Bacillus halodurans and 1 more | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin | 16.6 | 3.4 | 4.9 calc. | pH 7.5, 20 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin | 20.5 | 5.1 | 4.0 calc. | pH 7.5, 25 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin | 27.3 | 8.2 | 3.3 calc. | pH 7.5, 35 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin | 37.3 | 11.6 | 3.22 calc. | pH 7.5, 40 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoinisothermal titration calorimetry (ITC) assay | 24.9 | 7.9 | 3.2 | pH 7.5, 30 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin100 mM cacodylate buffer; 8 µM enzyme | 21.7 | 5.3 | 4.1 | pH 7.5, 30 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin50 mM cacodylate buffer; 30-50 µg protein | 24.7 | 6.6 | 3.7 | pH 7.5, 30 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 4.9 | 0.4 | 12 calc. | pH 7.5, 20 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 6.4 | 1.4 | 4.6 calc. | pH 7.5, 25 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 12.6 | 2.5 | 5.0 calc. | pH 7.5, 30 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 26 | 5.5 | 4.7 calc. | pH 7.5, 35 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 92.6 | 7 | 13 calc. | pH 7.5, 40 °C | PMID 16497383 | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.0055 | 0.1 | 0.055 calc. | pH 7.5, 20 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.02 | 0.23 | 0.087 calc. | pH 7.5, 25 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.061 | 0.43 | 0.14 calc. | pH 7.5, 30 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.22 | 2.6 | 0.085 calc. | pH 7.5, 35 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.44 | 3.7 | 0.12 calc. | pH 7.5, 40 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 0.6 | 0.4 | 1.5 calc. | pH 7.5, 20 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 1 | 0.9 | 1.1 calc. | pH 7.5, 25 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 3.9 | 1.2 | 3.2 calc. | pH 7.5, 30 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 7.7 | 2.6 | 3.0 calc. | pH 7.5, 35 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 11.4 | 4.7 | 2.4 calc. | pH 7.5, 40 °C | PMID 16497383 | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |
| D-hydantoinase (D-PfHYD) | Pseudomonas fluorescens | 5-(1H-indol-3-ylmethyl)imidazolidine-2,4-dione | 1.43 | 4.64 | 0.31 | pH 9, 45 °C | PMID 26821258 | Open record: D-hydantoinase (D-PfHYD), 5-(1H-indol-3-ylmethyl)imidazolidine-2,4-dione |
| D-hydantoinase (D-PfHYD) | Pseudomonas fluorescens | 5-(2-chlorophenyl)imidazolidine-2,4-dione | 0.37 | 8.57 | 0.04 | pH 9, 45 °C | PMID 26821258 | Open record: D-hydantoinase (D-PfHYD), 5-(2-chlorophenyl)imidazolidine-2,4-dione |
| D-hydantoinase (D-PfHYD) | Pseudomonas fluorescens | 5-(4-hydroxyphenyl)imidazolidine-2,4-dione | 74.2 | 7.52 | 9.87 | pH 9, 45 °C | PMID 26821258 | Open record: D-hydantoinase (D-PfHYD), 5-(4-hydroxyphenyl)imidazolidine-2,4-dione |
| D-hydantoinase (D-PfHYD) | Pseudomonas fluorescens | 5-benzylimidazolidine-2,4-dione | 2.91 | 9.49 | 0.31 | pH 9, 45 °C | PMID 26821258 | Open record: D-hydantoinase (D-PfHYD), 5-benzylimidazolidine-2,4-dione |
| D-hydantoinase (D-PfHYD) | Pseudomonas fluorescens | 5-isobutylimidazolidine-2,4-dione | 391 | 4.42 | 88.5 | pH 9, 45 °C | PMID 26821258 | Open record: D-hydantoinase (D-PfHYD), 5-isobutylimidazolidine-2,4-dione |
| D-hydantoinase (D-PfHYD) | Pseudomonas fluorescens | 5-isopropylimidazolidine-2,4-dione | 77.3 | 7.63 | 10.1 | pH 9, 45 °C | PMID 26821258 | Open record: D-hydantoinase (D-PfHYD), 5-isopropylimidazolidine-2,4-dione |
| D-hydantoinase AcDHTase | Anoxybacillus caldiproteolyticus | 3-isobutylglutarimide | 36.53 | 42.4 | 0.8612 | pH 10, 70 °C | PMID 41846534 | Open record: D-hydantoinase AcDHTase, 3-isobutylglutarimide |
| D-hydantoinase AcDHTasevariant M63A/F65H/C317T | Anoxybacillus caldiproteolyticus | 3-isobutylglutarimide | 18.03 | 24.73 | 0.7291 | pH 10, 70 °C | PMID 41846534 | Open record: D-hydantoinase AcDHTase variant M63A/F65H/C317T, 3-isobutylglutarimide |
| D-hydantoinase BhDHTase | Bacillus halodurans | 3-isobutylglutarimide | 19.84 | 43.77 | 0.4533 | pH 10, 80 °C | PMID 41846534 | Open record: D-hydantoinase BhDHTase, 3-isobutylglutarimide |
| D-hydantoinase BhDHTasevariant M63A/F65H/C317T | Bacillus halodurans | 3-isobutylglutarimide | 19.41 | 49.15 | 0.3949 | pH 10, 80 °C | PMID 41846534 | Open record: D-hydantoinase BhDHTase variant M63A/F65H/C317T, 3-isobutylglutarimide |
| D-hydantoinase GsDHTase | Geobacillus sp. | 3-isobutylglutarimide | 26.17 | 28.31 | 0.9244 | pH 10, 80 °C | PMID 41846534 | Open record: D-hydantoinase GsDHTase, 3-isobutylglutarimide |
| D-hydantoinase GsDHTasevariant M63A/F65H/C317T | Geobacillus sp. | 3-isobutylglutarimide | 34.21 | 57.73 | 0.5926 | pH 10, 80 °C | PMID 41846534 | Open record: D-hydantoinase GsDHTase variant M63A/F65H/C317T, 3-isobutylglutarimide |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | 5,6-dihydrouracil | 2 | 3 | 0.667 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), 5,6-dihydrouracil |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | D-5-(2-methylthioethyl)hydantoin | 18 | 66 | 0.273 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), D-5-(2-methylthioethyl)hydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | D-5-benzylhydantoin | 2 | 5 | 0.4 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), D-5-benzylhydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | D-5-butylhydantoin | 3 | 14 | 0.214 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), D-5-butylhydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | D-5-ethylhydantoin | 17 | 18 | 0.944 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), D-5-ethylhydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | D-5-isobutylhydantoin | 2 | 10 | 0.2 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), D-5-isobutylhydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | D-5-isopropylhydantoin | 5 | 44 | 0.114 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), D-5-isopropylhydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | D-5-methylhydantoin | 197 | 72 | 2.736 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), D-5-methylhydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | D-5-propylhydantoin | 6 | 26 | 0.231 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), D-5-propylhydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | DL-5-(2-methylthioethyl)hydantoin | 12 | 140 | 0.086 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), DL-5-(2-methylthioethyl)hydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | DL-5-(4-hydroxyphenyl)hydantoin | 1 | 20 | 0.05 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), DL-5-(4-hydroxyphenyl)hydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | DL-5-isopropylhydantoin | 5 | 87 | 0.057 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), DL-5-isopropylhydantoin |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | DL-5-methyl-5,6-dihydrouracil | 1 | 1 | 1 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), DL-5-methyl-5,6-dihydrouracil |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | DL-6-isopropyl-5,6-dihydrouracil | 1 | 81 | 0.012 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), DL-6-isopropyl-5,6-dihydrouracil |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | DL-6-methyl-5,6-dihydrouracil | 8 | 111 | 0.072 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), DL-6-methyl-5,6-dihydrouracil |
| dihydropyrimidinase (SmelDhp) | Sinorhizobium meliloti | imidazolidine-2,4-dione | 12 | 125 | 0.096 | pH 8, 40 °C | PMID 19895890 | Open record: dihydropyrimidinase (SmelDhp), imidazolidine-2,4-dione |