Values CatLog read from this paper
Enzymatic activity assay of D-hydantoinase by isothermal titration calorimetry. Determination of the thermodynamic activation parameters for the hydrolysis of several substrates
PMID 16497383doi:10.1016/j.jbbm.2006.01.002Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (47 values) from this paper: D-hydantoinase from Agrobacterium tumefaciens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 19 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin | 16.6 | 3.4 | 4.9 calc. | pH 7.5, 20 °C | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin | 20.5 | 5.1 | 4.0 calc. | pH 7.5, 25 °C | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin | 27.3 | 8.2 | 3.3 calc. | pH 7.5, 35 °C | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin | 37.3 | 11.6 | 3.22 calc. | pH 7.5, 40 °C | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoinisothermal titration calorimetry (ITC) assay | 24.9 | 7.9 | 3.2 | pH 7.5, 30 °C | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin100 mM cacodylate buffer; 8 µM enzyme | 21.7 | 5.3 | 4.1 | pH 7.5, 30 °C | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-ethyl-hydantoin50 mM cacodylate buffer; 30-50 µg protein | 24.7 | 6.6 | 3.7 | pH 7.5, 30 °C | Open record: D-hydantoinase, D-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 4.9 | 0.4 | 12 calc. | pH 7.5, 20 °C | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 6.4 | 1.4 | 4.6 calc. | pH 7.5, 25 °C | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 12.6 | 2.5 | 5.0 calc. | pH 7.5, 30 °C | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 26 | 5.5 | 4.7 calc. | pH 7.5, 35 °C | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | D-5-methylthioethyl-hydantoin | 92.6 | 7 | 13 calc. | pH 7.5, 40 °C | Open record: D-hydantoinase, D-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.0055 | 0.1 | 0.055 calc. | pH 7.5, 20 °C | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.02 | 0.23 | 0.087 calc. | pH 7.5, 25 °C | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.061 | 0.43 | 0.14 calc. | pH 7.5, 30 °C | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.22 | 2.6 | 0.085 calc. | pH 7.5, 35 °C | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-ethyl-hydantoin | 0.44 | 3.7 | 0.12 calc. | pH 7.5, 40 °C | Open record: D-hydantoinase, L-5-ethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 0.6 | 0.4 | 1.5 calc. | pH 7.5, 20 °C | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 1 | 0.9 | 1.1 calc. | pH 7.5, 25 °C | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 3.9 | 1.2 | 3.2 calc. | pH 7.5, 30 °C | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 7.7 | 2.6 | 3.0 calc. | pH 7.5, 35 °C | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |
| D-hydantoinase | Agrobacterium tumefaciens | L-5-methylthioethyl-hydantoin | 11.4 | 4.7 | 2.4 calc. | pH 7.5, 40 °C | Open record: D-hydantoinase, L-5-methylthioethyl-hydantoin |