EC 3.5.2.3: dihydroorotase
HydrolasesOpen in Browse
CatLog read 28 records for EC 3.5.2.3 from 3 papers, for 2 organisms. Names in these papers: dihydroorotase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 44 records for EC 3.5.2.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.2.3 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Mechanism of the dihydroorotase reaction | Escherichia coli | 12 |
| Activation of Latent Dihydroorotase from Aquifex aeolicus by Pressure | Aquifex aeolicus | 11 |
| Kinetic and structural analysis of mutant Escherichia coli dihydroorotases: a flexible loop stabilizes the transition state | Escherichia coli | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| dihydroorotase | Aquifex aeolicus | dihydroorotate35 bar | 1.7 | 5.33 | 0.32 calc. | pH 8, 25 °C | PMID 27746403 | Open record: dihydroorotase, dihydroorotate |
| dihydroorotase | Aquifex aeolicus | dihydroorotate70 bar | 3.13 | 4.41 | 0.710 calc. | pH 8, 25 °C | PMID 27746403 | Open record: dihydroorotase, dihydroorotate |
| dihydroorotase | Aquifex aeolicus | dihydroorotate175 bar | 6.7 | 5.74 | 1.2 calc. | pH 8, 25 °C | PMID 27746403 | Open record: dihydroorotase, dihydroorotate |
| dihydroorotase | Aquifex aeolicus | dihydroorotate350 bar | 9.57 | 6.65 | 1.44 calc. | pH 8, 25 °C | PMID 27746403 | Open record: dihydroorotase, dihydroorotate |
| dihydroorotase | Aquifex aeolicus | dihydroorotate700 bar | 10.1 | 6.71 | 1.51 calc. | pH 8, 25 °C | PMID 27746403 | Open record: dihydroorotase, dihydroorotate |
| dihydroorotase | Aquifex aeolicus | dihydroorotate | 62.3 | 2.38 | 26.2 calc. | pH 8.3, 74 °C | PMID 27746403 | Open record: dihydroorotase, dihydroorotate |
| dihydroorotasevariant C181G | Aquifex aeolicus | dihydroorotate | 15.5 | 3.89 | 3.98 calc. | pH 8.3, 74 °C | PMID 27746403 | Open record: dihydroorotase variant C181G, dihydroorotate |
| dihydroorotasevariant D179V | Aquifex aeolicus | dihydroorotateDHO-ATC complex | 9.2 | 0.24 | 38 calc. | pH 8.3, 74 °C | PMID 27746403 | Open record: dihydroorotase variant D179V, dihydroorotate |
| dihydroorotasevariant D179V | Aquifex aeolicus | dihydroorotateisolated DHO subunit (no ATC); isolated DHO subunit; no ATC | 3 | 6.2 | 0.48 calc. | pH 8.3, 74 °C | PMID 27746403 | Open record: dihydroorotase variant D179V, dihydroorotate |
| dihydroorotasevariant D183V | Aquifex aeolicus | dihydroorotateDHO-ATC complex | 9.7 | 0.47 | 21 calc. | pH 8.3, 74 °C | PMID 27746403 | Open record: dihydroorotase variant D183V, dihydroorotate |
| dihydroorotasevariant D183V | Aquifex aeolicus | dihydroorotateisolated DHO subunit (no ATC); isolated DHO subunit; no ATC | 6.7 | 24 | 0.28 calc. | pH 8.3, 74 °C | PMID 27746403 | Open record: dihydroorotase variant D183V, dihydroorotate |
| dihydroorotase | Escherichia coli | N-carbamoyl-DL-aspartate | 181.1 | 1.5 | 120 | pH 5.8, 30 °C | PMID 17711307 | Open record: dihydroorotase, N-carbamoyl-DL-aspartate |
| dihydroorotasevariant C63S/C65S/C121S/C179S | Escherichia coli | L-dihydroorotate | 100 | 0.08 | 1.2 × 10³ | pH 8 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S, L-dihydroorotate |
| dihydroorotasevariant C63S/C65S/C121S/C179S | Escherichia coli | L-dihydroorotate | 15 | 0.7 | 21 | pH 8 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S, L-dihydroorotate |
| dihydroorotasevariant C63S/C65S/C121S/C179S | Escherichia coli | L-dihydroorotate | 1.9 | 0.23 | 8.3 | pH 8 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S, L-dihydroorotate |
| dihydroorotasevariant C63S/C65S/C121S/C179S | Escherichia coli | N-carbamoyl-L-aspartate | 160 | 1.7 | 100 | pH 5.8 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S, N-carbamoyl-L-aspartate |
| dihydroorotasevariant C63S/C65S/C121S/C179S | Escherichia coli | N-carbamoyl-L-aspartate | 25 | 15 | 1.6 | pH 5.8 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S, N-carbamoyl-L-aspartate |
| dihydroorotasevariant C63S/C65S/C121S/C179S | Escherichia coli | N-carbamoyl-L-aspartate | 8.2 | 4 | 4.3 | pH 5.8 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S, N-carbamoyl-L-aspartate |
| dihydroorotasevariant C63S/C65S/C121S/C179S | Escherichia coli | N-carbamoyl-L-aspartate | 160 | 1.7 | 150 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S, N-carbamoyl-L-aspartate | |
| dihydroorotasevariant C63S/C65S/C121S/C179S | Escherichia coli | thiodihydroorotate | 4.4 | 0.03 | 150 | pH 8 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S, thiodihydroorotate |
| dihydroorotasevariant C63S/C65S/C121S/C179S | Escherichia coli | thiodihydroorotate | 0.42 | 0.009 | 48 | pH 8 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S, thiodihydroorotate |
| dihydroorotasevariant C63S/C65S/C121S/C179S/D250E | Escherichia coli | N-carbamoyl-L-aspartate | 12 | 1.9 | 6.3 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S/D250E, N-carbamoyl-L-aspartate | |
| dihydroorotasevariant C63S/C65S/C121S/C179S/D250S | Escherichia coli | N-carbamoyl-L-aspartate | 0.022 | 0.51 | 0.043 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S/D250S, N-carbamoyl-L-aspartate | |
| dihydroorotasevariant C63S/C65S/C121S/C179S/R20K | Escherichia coli | N-carbamoyl-L-aspartate | 15 | 0.9 | 17 | PMID 15610022 | Open record: dihydroorotase variant C63S/C65S/C121S/C179S/R20K, N-carbamoyl-L-aspartate | |
| dihydroorotasevariant T109S | Escherichia coli | N-carbamoyl-DL-aspartate | 49.8 | 6 | 8.3 | pH 5.8, 30 °C | PMID 17711307 | Open record: dihydroorotase variant T109S, N-carbamoyl-DL-aspartate |
| dihydroorotasevariant T110A | Escherichia coli | N-carbamoyl-DL-aspartate | 11.5 | 4 | 2.9 | pH 5.8, 30 °C | PMID 17711307 | Open record: dihydroorotase variant T110A, N-carbamoyl-DL-aspartate |
| dihydroorotasevariant T110S | Escherichia coli | N-carbamoyl-DL-aspartate | 59.7 | 3 | 20 | pH 5.8, 30 °C | PMID 17711307 | Open record: dihydroorotase variant T110S, N-carbamoyl-DL-aspartate |
| dihydroorotasevariant T110V | Escherichia coli | N-carbamoyl-DL-aspartate | 145.5 | 6 | 24 | pH 5.8, 30 °C | PMID 17711307 | Open record: dihydroorotase variant T110V, N-carbamoyl-DL-aspartate |