Values CatLog read from this paper
Kinetic and structural analysis of mutant Escherichia coli dihydroorotases: a flexible loop stabilizes the transition state
PMID 17711307doi:10.1021/bi701098eOpen in Browse
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CatLog read 5 records (15 values) from this paper: dihydroorotase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydroorotase | Escherichia coli | N-carbamoyl-DL-aspartate | 181.1 | 1.5 | 120 | pH 5.8, 30 °C | Open record: dihydroorotase, N-carbamoyl-DL-aspartate |
| dihydroorotasevariant T109S | Escherichia coli | N-carbamoyl-DL-aspartate | 49.8 | 6 | 8.3 | pH 5.8, 30 °C | Open record: dihydroorotase variant T109S, N-carbamoyl-DL-aspartate |
| dihydroorotasevariant T110A | Escherichia coli | N-carbamoyl-DL-aspartate | 11.5 | 4 | 2.9 | pH 5.8, 30 °C | Open record: dihydroorotase variant T110A, N-carbamoyl-DL-aspartate |
| dihydroorotasevariant T110S | Escherichia coli | N-carbamoyl-DL-aspartate | 59.7 | 3 | 20 | pH 5.8, 30 °C | Open record: dihydroorotase variant T110S, N-carbamoyl-DL-aspartate |
| dihydroorotasevariant T110V | Escherichia coli | N-carbamoyl-DL-aspartate | 145.5 | 6 | 24 | pH 5.8, 30 °C | Open record: dihydroorotase variant T110V, N-carbamoyl-DL-aspartate |