EC 3.5.5.1: nitrilase
HydrolasesOpen in Browse
CatLog read 74 records for EC 3.5.5.1 from 5 papers, for 8 organisms. Names in these papers: nitrilase, nitrilase (Nit6803), arylacetonitrilase nitA, nitrilase 1 (NIT1) and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 53 records for EC 3.5.5.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.5.1 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Purification and characterization of heterologously expressed nitrilases from filamentous fungi | Aspergillus niger, Gibberella moniliformis and 2 more | 19 |
| Enzymatic characterization of the recombinant Arabidopsis thaliana nitrilase subfamily encoded by the NIT2/NIT1/NIT3-gene cluster | Arabidopsis thaliana | 18 |
| Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Syechocystis sp. PCC6803 | Synechocystis sp. PCC 6803 | 18 |
| A positively charged amino acid at position 129 in nitrilase from Rhodococcus rhodochrous ATCC 33278 is an essential residue for the activity with meta-substituted benzonitriles | Rhodococcus rhodochrous | 11 |
| Improving catalytic performance of an arylacetonitrilase by semirational engineering | uncultured organism | 8 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| arylacetonitrilase nitA | uncultured organism | m-Chloromandelonitrile | 1.985 | 5.67 | 0.3497 | pH 7.5, 40 °C | PMID 28687857 | Open record: arylacetonitrilase nitA, m-Chloromandelonitrile |
| arylacetonitrilase nitA | uncultured organism | Mandelonitrile | 4.609 | 1.98 | 2.328 | pH 7.5, 40 °C | PMID 28687857 | Open record: arylacetonitrilase nitA, Mandelonitrile |
| arylacetonitrilase nitA | uncultured organism | o-Chloromandelonitrile | 1.319 | 3.98 | 0.3313 | pH 7.5, 40 °C | PMID 28687857 | Open record: arylacetonitrilase nitA, o-Chloromandelonitrile |
| arylacetonitrilase nitA | uncultured organism | p-Chloromandelonitrile | 2.21 | 6.15 | 0.3583 | pH 7.5, 40 °C | PMID 28687857 | Open record: arylacetonitrilase nitA, p-Chloromandelonitrile |
| arylacetonitrilase nitAvariant S190G | uncultured organism | m-Chloromandelonitrile | 3.415 | 5.47 | 0.6237 | pH 7.5, 40 °C | PMID 28687857 | Open record: arylacetonitrilase nitA variant S190G, m-Chloromandelonitrile |
| arylacetonitrilase nitAvariant S190G | uncultured organism | Mandelonitrile | 13.23 | 0.76 | 17.39 | pH 7.5, 40 °C | PMID 28687857 | Open record: arylacetonitrilase nitA variant S190G, Mandelonitrile |
| arylacetonitrilase nitAvariant S190G | uncultured organism | o-Chloromandelonitrile | 1.817 | 3.89 | 0.4663 | pH 7.5, 40 °C | PMID 28687857 | Open record: arylacetonitrilase nitA variant S190G, o-Chloromandelonitrile |
| arylacetonitrilase nitAvariant S190G | uncultured organism | p-Chloromandelonitrile | 3.069 | 6.08 | 0.5045 | pH 7.5, 40 °C | PMID 28687857 | Open record: arylacetonitrilase nitA variant S190G, p-Chloromandelonitrile |
| nitrilase | Aspergillus niger | (R,S)-2-phenylpropionitrile | — | 0.8 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, (R,S)-2-phenylpropionitrile |
| nitrilase | Aspergillus niger | (R,S)-Mandelonitrile | — | 11.4 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, (R,S)-Mandelonitrile |
| nitrilase | Aspergillus niger | Phenylacetonitrile | — | 3.4 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, Phenylacetonitrile |
| nitrilase | Gibberella moniliformis | 3-Chlorobenzonitrile | — | 0.93 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, 3-Chlorobenzonitrile |
| nitrilase | Gibberella moniliformis | 3-Cyanopyridine | — | 3.2 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, 3-Cyanopyridine |
| nitrilase | Gibberella moniliformis | 4-Chlorobenzonitrile | — | 1.3 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, 4-Chlorobenzonitrile |
| nitrilase | Gibberella moniliformis | 4-Cyanopyridine | — | 2.2 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, 4-Cyanopyridine |
| nitrilase | Gibberella moniliformis | Benzonitrile | — | 0.41 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, Benzonitrile |
| nitrilase | Gibberella moniliformis | Phenylacetonitrile | — | 0.75 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, Phenylacetonitrile |
| nitrilase | Neurospora crassa | (R,S)-2-phenylpropionitrile | — | 1.3 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, (R,S)-2-phenylpropionitrile |
| nitrilase | Neurospora crassa | (R,S)-Mandelonitrile | — | 3.4 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, (R,S)-Mandelonitrile |
| nitrilase | Neurospora crassa | Phenylacetonitrile | — | 2 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, Phenylacetonitrile |
| nitrilase | Penicillium marneffei | 2-Cyanopyridine | — | 11.7 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, 2-Cyanopyridine |
| nitrilase | Penicillium marneffei | 3-Chlorobenzonitrile | — | 11.1 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, 3-Chlorobenzonitrile |
| nitrilase | Penicillium marneffei | 3-Cyanopyridine | — | 34.4 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, 3-Cyanopyridine |
| nitrilase | Penicillium marneffei | 4-Chlorobenzonitrile | — | 2.6 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, 4-Chlorobenzonitrile |
| nitrilase | Penicillium marneffei | 4-Cyanopyridine | — | 15.4 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, 4-Cyanopyridine |
| nitrilase | Penicillium marneffei | Benzonitrile | — | 7.9 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, Benzonitrile |
| nitrilase | Penicillium marneffei | Phenylacetonitrile | — | 9.1 | — | pH 8, 30 °C | PMID 21892598 | Open record: nitrilase, Phenylacetonitrile |
| nitrilase | Rhodococcus rhodochrous | 3-aminobenzonitrile | 2.88 × 10⁻⁴ | 1.863 | 1.548 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase, 3-aminobenzonitrile | |
| nitrilase | Rhodococcus rhodochrous | 3-methylbenzonitrile | 1.98 × 10⁻⁴ | 1.851 | 1.068 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase, 3-methylbenzonitrile | |
| nitrilase | Rhodococcus rhodochrous | 4-aminobenzonitrile | 2.83 × 10⁻⁴ | 1.699 | 1.67 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase, 4-aminobenzonitrile | |
| nitrilase | Rhodococcus rhodochrous | 4-methylbenzonitrile | 6.95 × 10⁻⁴ | 2.426 | 2.87 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase, 4-methylbenzonitrile | |
| nitrilase | Rhodococcus rhodochrous | Benzonitrile | 1.293 × 10⁻⁴ | 1.068 | 1.223 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase, Benzonitrile | |
| nitrilasevariant R129H | Rhodococcus rhodochrous | 3-aminobenzonitrile | 1.53 × 10⁻⁴ | 0.483 | 3.18 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase variant R129H, 3-aminobenzonitrile | |
| nitrilasevariant R129H | Rhodococcus rhodochrous | 3-methylbenzonitrile | 1.78 × 10⁻⁴ | 0.592 | 3.03 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase variant R129H, 3-methylbenzonitrile | |
| nitrilasevariant R129H | Rhodococcus rhodochrous | Benzonitrile | 4.68 × 10⁻⁴ | 3.13 | 1.5 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase variant R129H, Benzonitrile | |
| nitrilasevariant R129K | Rhodococcus rhodochrous | 3-aminobenzonitrile | 1.4 × 10⁻⁴ | 0.456 | 3.08 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase variant R129K, 3-aminobenzonitrile | |
| nitrilasevariant R129K | Rhodococcus rhodochrous | 3-methylbenzonitrile | 1.58 × 10⁻⁴ | 0.55 | 2.9 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase variant R129K, 3-methylbenzonitrile | |
| nitrilasevariant R129K | Rhodococcus rhodochrous | Benzonitrile | 1.62 × 10⁻⁴ | 1.468 | 1.1 × 10⁻⁴ | PMID 19903476 | Open record: nitrilase variant R129K, Benzonitrile | |
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | 2-Butenenitrile | 0.19 | 7.95 | 0.0239 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803), 2-Butenenitrile |
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | 2-Cyanopyridine | 1.8 | 1.47 | 1.224 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803), 2-Cyanopyridine |
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | 3-Cyanopyridine | 0.45 | 3.2 | 0.1406 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803), 3-Cyanopyridine |
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | Benzonitrile | 0.65 | 1.21 | 0.5372 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803), Benzonitrile |
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | Fumaronitrile | 15.13 | 9.01 | 1.679 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803), Fumaronitrile |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | 2-Butenenitrile | 0.03 | 9.16 | 0.00328 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146F, 2-Butenenitrile |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | 2-Cyanopyridine | 1.95 | 1.26 | 1.548 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146F, 2-Cyanopyridine |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | 3-Cyanopyridine | 1.07 | 4.41 | 0.2426 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146F, 3-Cyanopyridine |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | Benzonitrile | 0.67 | 1.26 | 0.5317 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146F, Benzonitrile |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | Fumaronitrile | 8.75 | 11.45 | 0.7642 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146F, Fumaronitrile |
| nitrilase (Nit6803)variant W146H | Synechocystis sp. PCC 6803 | 2-Cyanopyridine | 1.81 | 1.3 | 1.392 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146H, 2-Cyanopyridine |
| nitrilase (Nit6803)variant W146H | Synechocystis sp. PCC 6803 | 3-Cyanopyridine | 1.21 | 3.94 | 0.3071 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146H, 3-Cyanopyridine |
| nitrilase (Nit6803)variant W146H | Synechocystis sp. PCC 6803 | Benzonitrile | 0.98 | 1.33 | 0.7368 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146H, Benzonitrile |
| nitrilase (Nit6803)variant W146H | Synechocystis sp. PCC 6803 | Fumaronitrile | 9.22 | 11.06 | 0.8336 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146H, Fumaronitrile |
| nitrilase (Nit6803)variant W146Y | Synechocystis sp. PCC 6803 | 2-Cyanopyridine | 2.05 | 1.13 | 1.814 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146Y, 2-Cyanopyridine |
| nitrilase (Nit6803)variant W146Y | Synechocystis sp. PCC 6803 | 3-Cyanopyridine | 1.13 | 3.16 | 0.3576 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146Y, 3-Cyanopyridine |
| nitrilase (Nit6803)variant W146Y | Synechocystis sp. PCC 6803 | Benzonitrile | 0.79 | 0.98 | 0.8061 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146Y, Benzonitrile |
| nitrilase (Nit6803)variant W146Y | Synechocystis sp. PCC 6803 | Fumaronitrile | 7.42 | 10.77 | 0.6889 | pH 7, 30 °C | PMID 25450592 | Open record: nitrilase (Nit6803) variant W146Y, Fumaronitrile |
| nitrilase 1 (NIT1) | Arabidopsis thaliana | (methylthio)acetonitrile | — | 0.88 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 1 (NIT1), (methylthio)acetonitrile |
| nitrilase 1 (NIT1) | Arabidopsis thaliana | 3-phenylpropionitrile | — | 0.22 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 1 (NIT1), 3-phenylpropionitrile |
| nitrilase 1 (NIT1) | Arabidopsis thaliana | Allylcyanide | — | 0.6 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 1 (NIT1), Allylcyanide |
| nitrilase 1 (NIT1) | Arabidopsis thaliana | indole-3-acetonitrile | — | 11.1 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 1 (NIT1), indole-3-acetonitrile |
| nitrilase 1 (NIT1) | Arabidopsis thaliana | Phenylacetonitrile | — | 0.14 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 1 (NIT1), Phenylacetonitrile |
| nitrilase 1 (NIT1) | Arabidopsis thaliana | Phenylbutyronitrile | — | 0.08 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 1 (NIT1), Phenylbutyronitrile |
| nitrilase 2 (NIT2) | Arabidopsis thaliana | (methylthio)acetonitrile | — | 1.09 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 2 (NIT2), (methylthio)acetonitrile |
| nitrilase 2 (NIT2) | Arabidopsis thaliana | 3-phenylpropionitrile | — | 0.43 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 2 (NIT2), 3-phenylpropionitrile |
| nitrilase 2 (NIT2) | Arabidopsis thaliana | Allylcyanide | — | 6.27 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 2 (NIT2), Allylcyanide |
| nitrilase 2 (NIT2) | Arabidopsis thaliana | indole-3-acetonitrile | — | 7.4 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 2 (NIT2), indole-3-acetonitrile |
| nitrilase 2 (NIT2) | Arabidopsis thaliana | Phenylacetonitrile | — | 1 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 2 (NIT2), Phenylacetonitrile |
| nitrilase 2 (NIT2) | Arabidopsis thaliana | Phenylbutyronitrile | — | 0.09 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 2 (NIT2), Phenylbutyronitrile |
| nitrilase 3 (NIT3) | Arabidopsis thaliana | (methylthio)acetonitrile | — | 1.31 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 3 (NIT3), (methylthio)acetonitrile |
| nitrilase 3 (NIT3) | Arabidopsis thaliana | 3-phenylpropionitrile | — | 0.42 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 3 (NIT3), 3-phenylpropionitrile |
| nitrilase 3 (NIT3) | Arabidopsis thaliana | Allylcyanide | — | 2.09 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 3 (NIT3), Allylcyanide |
| nitrilase 3 (NIT3) | Arabidopsis thaliana | indole-3-acetonitrile | — | 30.1 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 3 (NIT3), indole-3-acetonitrile |
| nitrilase 3 (NIT3) | Arabidopsis thaliana | Phenylacetonitrile | — | 0.23 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 3 (NIT3), Phenylacetonitrile |
| nitrilase 3 (NIT3) | Arabidopsis thaliana | Phenylbutyronitrile | — | 0.07 | — | pH 8, 30 °C | PMID 11525507 | Open record: nitrilase 3 (NIT3), Phenylbutyronitrile |