Values CatLog read from this paper
Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Syechocystis sp. PCC6803
PMID 25450592doi:10.1016/j.jsb.2014.10.003Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (54 values) from this paper: nitrilase (Nit6803) from Synechocystis sp. PCC 6803.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | 2-Butenenitrile | 0.19 | 7.95 | 0.0239 | pH 7, 30 °C | Open record: nitrilase (Nit6803), 2-Butenenitrile |
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | 2-Cyanopyridine | 1.8 | 1.47 | 1.224 | pH 7, 30 °C | Open record: nitrilase (Nit6803), 2-Cyanopyridine |
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | 3-Cyanopyridine | 0.45 | 3.2 | 0.1406 | pH 7, 30 °C | Open record: nitrilase (Nit6803), 3-Cyanopyridine |
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | Benzonitrile | 0.65 | 1.21 | 0.5372 | pH 7, 30 °C | Open record: nitrilase (Nit6803), Benzonitrile |
| nitrilase (Nit6803) | Synechocystis sp. PCC 6803 | Fumaronitrile | 15.13 | 9.01 | 1.679 | pH 7, 30 °C | Open record: nitrilase (Nit6803), Fumaronitrile |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | 2-Butenenitrile | 0.03 | 9.16 | 0.00328 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146F, 2-Butenenitrile |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | 2-Cyanopyridine | 1.95 | 1.26 | 1.548 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146F, 2-Cyanopyridine |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | 3-Cyanopyridine | 1.07 | 4.41 | 0.2426 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146F, 3-Cyanopyridine |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | Benzonitrile | 0.67 | 1.26 | 0.5317 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146F, Benzonitrile |
| nitrilase (Nit6803)variant W146F | Synechocystis sp. PCC 6803 | Fumaronitrile | 8.75 | 11.45 | 0.7642 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146F, Fumaronitrile |
| nitrilase (Nit6803)variant W146H | Synechocystis sp. PCC 6803 | 2-Cyanopyridine | 1.81 | 1.3 | 1.392 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146H, 2-Cyanopyridine |
| nitrilase (Nit6803)variant W146H | Synechocystis sp. PCC 6803 | 3-Cyanopyridine | 1.21 | 3.94 | 0.3071 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146H, 3-Cyanopyridine |
| nitrilase (Nit6803)variant W146H | Synechocystis sp. PCC 6803 | Benzonitrile | 0.98 | 1.33 | 0.7368 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146H, Benzonitrile |
| nitrilase (Nit6803)variant W146H | Synechocystis sp. PCC 6803 | Fumaronitrile | 9.22 | 11.06 | 0.8336 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146H, Fumaronitrile |
| nitrilase (Nit6803)variant W146Y | Synechocystis sp. PCC 6803 | 2-Cyanopyridine | 2.05 | 1.13 | 1.814 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146Y, 2-Cyanopyridine |
| nitrilase (Nit6803)variant W146Y | Synechocystis sp. PCC 6803 | 3-Cyanopyridine | 1.13 | 3.16 | 0.3576 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146Y, 3-Cyanopyridine |
| nitrilase (Nit6803)variant W146Y | Synechocystis sp. PCC 6803 | Benzonitrile | 0.79 | 0.98 | 0.8061 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146Y, Benzonitrile |
| nitrilase (Nit6803)variant W146Y | Synechocystis sp. PCC 6803 | Fumaronitrile | 7.42 | 10.77 | 0.6889 | pH 7, 30 °C | Open record: nitrilase (Nit6803) variant W146Y, Fumaronitrile |