EC 3.5.5.7: Aliphatic nitrilase

HydrolasesOpen in Browse

CatLog read 39 records for EC 3.5.5.7 from 2 papers, for 2 organisms. Names in these papers: nitrilase, nitrilase (NitPf5).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 26 records for EC 3.5.5.7 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.5.7 in Browse.

Papers

Papers with records for EC 3.5.5.7
Paper	Organisms	Records
An amino acid at position 142 in nitrilase from Rhodococcus rhodochrous ATCC 33278 determines the substrate specificity for aliphatic and aromatic nitriles	Rhodococcus rhodochrous	28
Identification and characterization of a novel nitrilase from Pseudomonas fluorescens Pf-5	Pseudomonas fluorescens	11

Records

Records for EC 3.5.5.7 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
nitrilase	Rhodococcus rhodochrous	2-Cyanopyridine	1.77 × 10⁻⁴	1.53	1.15 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase, 2-Cyanopyridine
nitrilase	Rhodococcus rhodochrous	Adiponitrile	4.6 × 10⁻⁴	0.933	4.93 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase, Adiponitrile
nitrilase	Rhodococcus rhodochrous	Benzonitrile	1.57 × 10⁻⁴	1.07	1.47 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase, Benzonitrile
nitrilase	Rhodococcus rhodochrous	Glutaronitrile	2.88 × 10⁻⁴	1.24	2.33 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase, Glutaronitrile
nitrilase	Rhodococcus rhodochrous	m-Tolunitrile	2.38 × 10⁻⁴	1.85	1.28 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase, m-Tolunitrile
nitrilasevariant K9A	Rhodococcus rhodochrous	2-Cyanopyridine	1.88 × 10⁻⁴	1.25	1.5 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant K9A, 2-Cyanopyridine
nitrilasevariant K9A	Rhodococcus rhodochrous	Adiponitrile	5.72 × 10⁻⁴	0.851	6.73 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant K9A, Adiponitrile
nitrilasevariant K9A	Rhodococcus rhodochrous	Benzonitrile	1.57 × 10⁻⁴	1.02	1.53 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant K9A, Benzonitrile
nitrilasevariant K9A	Rhodococcus rhodochrous	Glutaronitrile	3.27 × 10⁻⁴	1.19	2.75 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant K9A, Glutaronitrile
nitrilasevariant K9A	Rhodococcus rhodochrous	m-Tolunitrile	1.75 × 10⁻⁴	1.62	1.08 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant K9A, m-Tolunitrile
nitrilasevariant L79A	Rhodococcus rhodochrous	2-Cyanopyridine	2.52 × 10⁻⁴	1.5	1.68 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant L79A, 2-Cyanopyridine
nitrilasevariant L79A	Rhodococcus rhodochrous	Adiponitrile	4.97 × 10⁻⁴	0.921	5.4 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant L79A, Adiponitrile
nitrilasevariant L79A	Rhodococcus rhodochrous	Benzonitrile	2.1 × 10⁻⁴	0.91	2.3 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant L79A, Benzonitrile
nitrilasevariant L79A	Rhodococcus rhodochrous	Glutaronitrile	3.15 × 10⁻⁴	1.15	2.72 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant L79A, Glutaronitrile
nitrilasevariant L79A	Rhodococcus rhodochrous	m-Tolunitrile	2.77 × 10⁻⁴	1.79	1.55 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant L79A, m-Tolunitrile
nitrilasevariant Y142A	Rhodococcus rhodochrous	2-Cyanopyridine	3.08 × 10⁻⁴	0.819	3.77 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142A, 2-Cyanopyridine
nitrilasevariant Y142A	Rhodococcus rhodochrous	Benzonitrile	2.88 × 10⁻⁴	1.58	1.82 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142A, Benzonitrile
nitrilasevariant Y142A	Rhodococcus rhodochrous	m-Tolunitrile	1.88 × 10⁻⁴	0.612	3.08 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142A, m-Tolunitrile
nitrilasevariant Y142F	Rhodococcus rhodochrous	2-Cyanopyridine	1.18 × 10⁻⁴	1.56	7.7 × 10⁻⁵	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142F, 2-Cyanopyridine
nitrilasevariant Y142F	Rhodococcus rhodochrous	Adiponitrile	1.77 × 10⁻⁴	0.688	2.57 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142F, Adiponitrile
nitrilasevariant Y142F	Rhodococcus rhodochrous	Benzonitrile	2.48 × 10⁻⁴	3.45	7.2 × 10⁻⁵	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142F, Benzonitrile
nitrilasevariant Y142F	Rhodococcus rhodochrous	Glutaronitrile	1.77 × 10⁻⁴	1.56	1.13 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142F, Glutaronitrile
nitrilasevariant Y142F	Rhodococcus rhodochrous	m-Tolunitrile	1.42 × 10⁻⁴	1.19	1.18 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142F, m-Tolunitrile
nitrilasevariant Y142S	Rhodococcus rhodochrous	2-Cyanopyridine	7.33 × 10⁻⁴	3.75	1.95 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142S, 2-Cyanopyridine
nitrilasevariant Y142S	Rhodococcus rhodochrous	Benzonitrile	4.3 × 10⁻⁴	1.82	2.37 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142S, Benzonitrile
nitrilasevariant Y142S	Rhodococcus rhodochrous	m-Tolunitrile	3.77 × 10⁻⁴	1.49	2.53 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142S, m-Tolunitrile
nitrilasevariant Y142V	Rhodococcus rhodochrous	Benzonitrile	1.38 × 10⁻⁴	0.49	2.82 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142V, Benzonitrile
nitrilasevariant Y142V	Rhodococcus rhodochrous	m-Tolunitrile	1.02 × 10⁻⁴	0.47	2.2 × 10⁻⁴	pH 7.5, 25 °C	PMID 18412544	Open record: nitrilase variant Y142V, m-Tolunitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	(E)-but-2-enedinitrile	9.2	23	0.4	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), (E)-but-2-enedinitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	1-Chlorobenzonitrile	0.17	35.2	0.005	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), 1-Chlorobenzonitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	2-Thiopheneacetonitrile	0.24	29.5	0.008	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), 2-Thiopheneacetonitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	Acrylonitrile	0.33	23.3	0.014	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), Acrylonitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	Benzonitrile	0.22	23.8	0.009	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), Benzonitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	butanedinitrile	8.8	17.9	0.5	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), butanedinitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	decanedinitrile	3.3	25.3	0.16	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), decanedinitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	n-Butyronitrile	0.2	35.3	0.006	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), n-Butyronitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	pentanedinitrile	5	24	0.25	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), pentanedinitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	Phenylacetonitrile	0.49	35.2	0.014	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), Phenylacetonitrile
nitrilase (NitPf5)	Pseudomonas fluorescens	Propionitrile	0.17	43.9	0.004	pH 7, 30 °C	PMID 19153727	Open record: nitrilase (NitPf5), Propionitrile