Values CatLog read from this paper

An amino acid at position 142 in nitrilase from Rhodococcus rhodochrous ATCC 33278 determines the substrate specificity for aliphatic and aromatic nitriles

PMID 18412544doi:10.1042/bj20080440Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (84 values) from this paper: nitrilase from Rhodococcus rhodochrous.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.5.7

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
nitrilase	Rhodococcus rhodochrous	2-Cyanopyridine	1.77 × 10⁻⁴	1.53	1.15 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase, 2-Cyanopyridine
nitrilase	Rhodococcus rhodochrous	Adiponitrile	4.6 × 10⁻⁴	0.933	4.93 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase, Adiponitrile
nitrilase	Rhodococcus rhodochrous	Benzonitrile	1.57 × 10⁻⁴	1.07	1.47 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase, Benzonitrile
nitrilase	Rhodococcus rhodochrous	Glutaronitrile	2.88 × 10⁻⁴	1.24	2.33 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase, Glutaronitrile
nitrilase	Rhodococcus rhodochrous	m-Tolunitrile	2.38 × 10⁻⁴	1.85	1.28 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase, m-Tolunitrile
nitrilasevariant K9A	Rhodococcus rhodochrous	2-Cyanopyridine	1.88 × 10⁻⁴	1.25	1.5 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant K9A, 2-Cyanopyridine
nitrilasevariant K9A	Rhodococcus rhodochrous	Adiponitrile	5.72 × 10⁻⁴	0.851	6.73 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant K9A, Adiponitrile
nitrilasevariant K9A	Rhodococcus rhodochrous	Benzonitrile	1.57 × 10⁻⁴	1.02	1.53 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant K9A, Benzonitrile
nitrilasevariant K9A	Rhodococcus rhodochrous	Glutaronitrile	3.27 × 10⁻⁴	1.19	2.75 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant K9A, Glutaronitrile
nitrilasevariant K9A	Rhodococcus rhodochrous	m-Tolunitrile	1.75 × 10⁻⁴	1.62	1.08 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant K9A, m-Tolunitrile
nitrilasevariant L79A	Rhodococcus rhodochrous	2-Cyanopyridine	2.52 × 10⁻⁴	1.5	1.68 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant L79A, 2-Cyanopyridine
nitrilasevariant L79A	Rhodococcus rhodochrous	Adiponitrile	4.97 × 10⁻⁴	0.921	5.4 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant L79A, Adiponitrile
nitrilasevariant L79A	Rhodococcus rhodochrous	Benzonitrile	2.1 × 10⁻⁴	0.91	2.3 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant L79A, Benzonitrile
nitrilasevariant L79A	Rhodococcus rhodochrous	Glutaronitrile	3.15 × 10⁻⁴	1.15	2.72 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant L79A, Glutaronitrile
nitrilasevariant L79A	Rhodococcus rhodochrous	m-Tolunitrile	2.77 × 10⁻⁴	1.79	1.55 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant L79A, m-Tolunitrile
nitrilasevariant Y142A	Rhodococcus rhodochrous	2-Cyanopyridine	3.08 × 10⁻⁴	0.819	3.77 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142A, 2-Cyanopyridine
nitrilasevariant Y142A	Rhodococcus rhodochrous	Benzonitrile	2.88 × 10⁻⁴	1.58	1.82 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142A, Benzonitrile
nitrilasevariant Y142A	Rhodococcus rhodochrous	m-Tolunitrile	1.88 × 10⁻⁴	0.612	3.08 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142A, m-Tolunitrile
nitrilasevariant Y142F	Rhodococcus rhodochrous	2-Cyanopyridine	1.18 × 10⁻⁴	1.56	7.7 × 10⁻⁵	pH 7.5, 25 °C	Open record: nitrilase variant Y142F, 2-Cyanopyridine
nitrilasevariant Y142F	Rhodococcus rhodochrous	Adiponitrile	1.77 × 10⁻⁴	0.688	2.57 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142F, Adiponitrile
nitrilasevariant Y142F	Rhodococcus rhodochrous	Benzonitrile	2.48 × 10⁻⁴	3.45	7.2 × 10⁻⁵	pH 7.5, 25 °C	Open record: nitrilase variant Y142F, Benzonitrile
nitrilasevariant Y142F	Rhodococcus rhodochrous	Glutaronitrile	1.77 × 10⁻⁴	1.56	1.13 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142F, Glutaronitrile
nitrilasevariant Y142F	Rhodococcus rhodochrous	m-Tolunitrile	1.42 × 10⁻⁴	1.19	1.18 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142F, m-Tolunitrile
nitrilasevariant Y142S	Rhodococcus rhodochrous	2-Cyanopyridine	7.33 × 10⁻⁴	3.75	1.95 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142S, 2-Cyanopyridine
nitrilasevariant Y142S	Rhodococcus rhodochrous	Benzonitrile	4.3 × 10⁻⁴	1.82	2.37 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142S, Benzonitrile
nitrilasevariant Y142S	Rhodococcus rhodochrous	m-Tolunitrile	3.77 × 10⁻⁴	1.49	2.53 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142S, m-Tolunitrile
nitrilasevariant Y142V	Rhodococcus rhodochrous	Benzonitrile	1.38 × 10⁻⁴	0.49	2.82 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142V, Benzonitrile
nitrilasevariant Y142V	Rhodococcus rhodochrous	m-Tolunitrile	1.02 × 10⁻⁴	0.47	2.2 × 10⁻⁴	pH 7.5, 25 °C	Open record: nitrilase variant Y142V, m-Tolunitrile