EC 3.6.1.5: apyrase
HydrolasesOpen in Browse
CatLog read 56 records for EC 3.6.1.5 from 3 papers, for 3 organisms. Names in these papers: NTPDase1 (CD39), apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, TgNTPDase1 and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 98 records for EC 3.6.1.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.6.1.5 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Crystallographic evidence for a domain motion in rat nucleoside triphosphate diphosphohydrolase (NTPDase) 1 | Rattus norvegicus | 26 |
| Potato tuber isoapyrases: substrate specificity, affinity labeling, and proteolytic susceptibility | Solanum tuberosum | 22 |
| The ATP/ADP substrate specificity switch between Toxoplasma gondii NTPDase1 and NTPDase3 is caused by an altered mode of binding of the substrate base | Toxoplasma gondii | 8 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 1,N6-ethenoadenosine 5'-diphosphate | 1384 | 0.073 | 2 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 1,N6-ethenoadenosine 5'-diphosphate |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 1,N6-ethenoadenosine 5'-triphosphate | 1642 | 0.031 | 5.3 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 1,N6-ethenoadenosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 2'(3')-O-(N-methylanthraniloyl)adenosine 5'-diphosphate | 828 | 0.014 | 5.91 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 2'(3')-O-(N-methylanthraniloyl)adenosine 5'-diphosphate |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 2'(3')-O-(N-methylanthraniloyl)adenosine 5'-triphosphate | 965 | 0.012 | 8.04 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 2'(3')-O-(N-methylanthraniloyl)adenosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate | 328 | 0.019 | 1.73 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate | 982 | 0.018 | 5.5 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 2'-deoxyadenosine 5'-O-(1-thiotriphosphate) | 1101 | 0.021 | 5.24 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 2'-deoxyadenosine 5'-O-(1-thiotriphosphate) |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 2'-deoxyadenosine 5'-triphosphate | 2222 | 0.018 | 1.234 × 10⁵ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 2'-deoxyadenosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 2'-deoxycytidine 5'-triphosphate | 2174 | 0.029 | 7.5 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 2'-deoxycytidine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 2'-deoxyguanosine 5'-triphosphate | 3019 | 0.028 | 1.078 × 10⁵ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 2'-deoxyguanosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Desirée isoapyrase | Solanum tuberosum | 2'-deoxythymidine 5'-triphosphate | 2841 | 0.027 | 1.052 × 10⁵ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Desirée isoapyrase, 2'-deoxythymidine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 1,N6-ethenoadenosine 5'-diphosphate | 86 | 0.114 | 800 | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 1,N6-ethenoadenosine 5'-diphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 1,N6-ethenoadenosine 5'-triphosphate | 682 | 0.024 | 2.84 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 1,N6-ethenoadenosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 2'(3')-O-(N-methylanthraniloyl)adenosine 5'-diphosphate | 23 | 0.017 | 1.4 × 10³ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 2'(3')-O-(N-methylanthraniloyl)adenosine 5'-diphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 2'(3')-O-(N-methylanthraniloyl)adenosine 5'-triphosphate | 800 | 0.018 | 4.44 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 2'(3')-O-(N-methylanthraniloyl)adenosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate | 70 | 0.009 | 7.8 × 10³ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate | 191 | 0.008 | 2.39 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 2'-deoxyadenosine 5'-O-(1-thiotriphosphate) | 595 | 0.048 | 1.24 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 2'-deoxyadenosine 5'-O-(1-thiotriphosphate) |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 2'-deoxyadenosine 5'-triphosphate | 636 | 0.031 | 2.05 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 2'-deoxyadenosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 2'-deoxycytidine 5'-triphosphate | 490 | 0.032 | 1.53 × 10⁴ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 2'-deoxycytidine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 2'-deoxyguanosine 5'-triphosphate | 573 | 0.133 | 4.3 × 10³ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 2'-deoxyguanosine 5'-triphosphate |
| apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase | Solanum tuberosum | 2'-deoxythymidine 5'-triphosphate | 455 | 0.093 | 4.9 × 10³ | pH 6, 30 °C | PMID 15896365 | Open record: apyrase (ATP-diphosphohydrolase), Pimpernel isoapyrase, 2'-deoxythymidine 5'-triphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | adenosine 5'-diphosphate | 77.3 | 0.0047 | 1.64 × 10⁴ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), adenosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | adenosine 5′-[(α,β)-methyleno] triphosphate | 40.5 | — | — | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), adenosine 5′-[(α,β)-methyleno] triphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphate | — | 0.0114 | — | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphate | 83.2 | — | — | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphate | — | — | 7.3 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphatein presence of 100 μM AMP (inhibitor); apparent Km | — | 0.0488 | — | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphatein presence of 100 μM AMP (inhibitor); apparent kcat | 82.6 | — | — | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphatein presence of 100 μM AMP (inhibitor); apparent kcat/Km | — | — | 1.7 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphatein presence of 100 μM UMP (inhibitor); apparent Km | — | 0.0131 | — | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphatein presence of 100 μM UMP (inhibitor); apparent kcat | 83.2 | — | — | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphatein presence of 100 μM UMP (inhibitor); apparent kcat/Km | — | — | 6.4 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphatein presence of 100 μM AHM (inhibitor); apparent Km | — | 0.149 | — | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphatein presence of 100 μM AHM (inhibitor); apparent kcat | 103.7 | — | — | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-diphosphatein presence of 100 μM AHM (inhibitor); apparent kcat/Km | — | — | 700 | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | guanosine 5'-triphosphate | 80.9 | 0.009 | 9 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), guanosine 5'-triphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | inosine 5'-diphosphate | 79.9 | 0.0105 | 7.6 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), inosine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | inosine 5'-triphosphate | 105.3 | 0.0108 | 9.8 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), inosine 5'-triphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | thiamine pyrophosphate | 80.2 | 0.7495 | 110 | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), thiamine pyrophosphate |
| NTPDase1 (CD39) | Rattus norvegicus | uridine 5'-diphosphate | 80.9 | 0.0113 | 7.2 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), uridine 5'-diphosphate |
| NTPDase1 (CD39) | Rattus norvegicus | uridine 5'-triphosphate | 103.4 | 0.01 | 1.03 × 10⁴ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39), uridine 5'-triphosphate |
| NTPDase1 (CD39)variant K257M | Rattus norvegicus | adenosine 5'-diphosphate | 50.4 | 0.0025 | 2.02 × 10⁴ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39) variant K257M, adenosine 5'-diphosphate |
| NTPDase1 (CD39)variant Y409F | Rattus norvegicus | adenosine 5'-diphosphate | 31.1 | 0.0051 | 6.1 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39) variant Y409F, adenosine 5'-diphosphate |
| NTPDase1 (CD39)variant Y413F | Rattus norvegicus | adenosine 5'-diphosphate | 86.1 | 0.0032 | 2.69 × 10⁴ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39) variant Y413F, adenosine 5'-diphosphate |
| NTPDase1 (CD39)variant ΔMIL (T190–A206 replaced by KTPGGS) | Rattus norvegicus | adenosine 5'-diphosphate | 86.9 | 0.0059 | 1.47 × 10⁴ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39) variant ΔMIL (T190–A206 replaced by KTPGGS), adenosine 5'-diphosphate |
| NTPDase1 (CD39)variant ΔMIL (T190–A206 replaced by KTPGGS) | Rattus norvegicus | uridine 5'-diphosphate | 93 | 0.0175 | 5.3 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39) variant ΔMIL (T190–A206 replaced by KTPGGS), uridine 5'-diphosphate |
| NTPDase1 (CD39)variant ΔMIL (T190–A206 replaced by KTPGGS) | Rattus norvegicus | uridine 5'-triphosphate | 121.2 | 0.0129 | 9.4 × 10³ | pH 7.4, 25 °C | PMID 22100451 | Open record: NTPDase1 (CD39) variant ΔMIL (T190–A206 replaced by KTPGGS), uridine 5'-triphosphate |
| TgNTPDase1variant C258S/C268S/E493G (TgNTPDase1-RG) | Toxoplasma gondii | adenosine 5'-diphosphate | 109 | 0.322 | 340 | pH 7.4 | PMID 24115522 | Open record: TgNTPDase1 variant C258S/C268S/E493G (TgNTPDase1-RG), adenosine 5'-diphosphate |
| TgNTPDase1variant C258S/C268S/E493G (TgNTPDase1-RG) | Toxoplasma gondii | adenosine 5'-triphosphate | 1.41 × 10³ | 0.084 | 1.7 × 10⁴ | pH 7.4 | PMID 24115522 | Open record: TgNTPDase1 variant C258S/C268S/E493G (TgNTPDase1-RG), adenosine 5'-triphosphate |
| TgNTPDase1variant C258S/C268S/R492G (TgNTPDase1-GE) | Toxoplasma gondii | adenosine 5'-diphosphate | 570 | 2.1 | 270 | pH 7.4 | PMID 24115522 | Open record: TgNTPDase1 variant C258S/C268S/R492G (TgNTPDase1-GE), adenosine 5'-diphosphate |
| TgNTPDase1variant C258S/C268S/R492G (TgNTPDase1-GE) | Toxoplasma gondii | adenosine 5'-triphosphate | 1.23 × 10³ | 2.53 | 490 | pH 7.4 | PMID 24115522 | Open record: TgNTPDase1 variant C258S/C268S/R492G (TgNTPDase1-GE), adenosine 5'-triphosphate |
| TgNTPDase1variant C258S/C268S/R492G/E493G (TgNTPDase1-GG) | Toxoplasma gondii | adenosine 5'-diphosphate | 93 | 0.36 | 254 | pH 7.4 | PMID 24115522 | Open record: TgNTPDase1 variant C258S/C268S/R492G/E493G (TgNTPDase1-GG), adenosine 5'-diphosphate |
| TgNTPDase1variant C258S/C268S/R492G/E493G (TgNTPDase1-GG) | Toxoplasma gondii | adenosine 5'-triphosphate | 2.2 × 10³ | 0.12 | 1.9 × 10⁴ | pH 7.4 | PMID 24115522 | Open record: TgNTPDase1 variant C258S/C268S/R492G/E493G (TgNTPDase1-GG), adenosine 5'-triphosphate |
| TgNTPDase3variant C258S/C268S/G492R/G493E (TgNTPDase3-RE) | Toxoplasma gondii | adenosine 5'-diphosphate | 67 | 1.25 | 54 | pH 7.4 | PMID 24115522 | Open record: TgNTPDase3 variant C258S/C268S/G492R/G493E (TgNTPDase3-RE), adenosine 5'-diphosphate |
| TgNTPDase3variant C258S/C268S/G492R/G493E (TgNTPDase3-RE) | Toxoplasma gondii | adenosine 5'-triphosphate | 222 | 2.46 | 90 | pH 7.4 | PMID 24115522 | Open record: TgNTPDase3 variant C258S/C268S/G492R/G493E (TgNTPDase3-RE), adenosine 5'-triphosphate |