EC 3.6.5.3: protein-synthesizing GTPase
HydrolasesOpen in Browse
CatLog read 16 records for EC 3.6.5.3 from 2 papers, for 1 organism. Names in these papers: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), elongation factor 2 (SsEF-2), intrinsic GTPase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 37 records for EC 3.6.5.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.6.5.3 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| The site for GTP hydrolysis on the archaeal elongation factor 2 is unmasked by aliphatic alcohols | Sulfolobus solfataricus | 11 |
| The A26G replacement in the consensus sequence A-X-X-X-X-G-K-[T,S] of the guanine nucleotide binding site activates the intrinsic GTPase of the elongation factor 2 from the archaeon Sulfolobus… | Sulfolobus solfataricus | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| elongation factor 2 (SsEF-2), intrinsic GTPase | Sulfolobus solfataricus | guanosine 5'-triphosphate10% (v/v) ethylene glycol | 0.0033 | 0.0096 | 0.33 | pH 7.5, 60 °C | PMID 10336648 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase, guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase | Sulfolobus solfataricus | guanosine 5'-triphosphate40% (v/v) ethylene glycol | 0.032 | 0.001 | 32 | pH 7.5, 60 °C | PMID 10336648 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase, guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPasevariant A26G | Sulfolobus solfataricus | guanosine 5'-triphosphateno ethylene glycol (0%) | 0.133 | 0.0194 | 6.7 | pH 7.5, 60 °C | PMID 10336648 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase variant A26G, guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPasevariant A26G | Sulfolobus solfataricus | guanosine 5'-triphosphate10% (v/v) ethylene glycol | 0.12 | 0.0055 | 22 | pH 7.5, 60 °C | PMID 10336648 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase variant A26G, guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPasevariant A26G | Sulfolobus solfataricus | guanosine 5'-triphosphate40% (v/v) ethylene glycol | 0.165 | 8 × 10⁻⁴ | 207 | pH 7.5, 60 °C | PMID 10336648 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase variant A26G, guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.023 | 9 × 10⁻⁴ | 26 | pH 7.8, 50 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate20-50 pmol SsEF-2 in 200 µL; 0.5-200 µM [gamma-32P]GTP | 0.038 | 0.0012 | 32 | pH 7.8, 60 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.118 | 0.0014 | 84 | pH 7.8, 70 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.152 | 0.001 | 152 | pH 7.8, 75 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.23 | 0.0013 | 177 | pH 7.8, 80 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.512 | 0.0053 | 97 | pH 7.8, 87 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.89 | 0.02 | 45 | pH 7.8, 91 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.558 | 0.0532 | 11 | pH 7.8, 95 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphateno ribosome (GTPaseg); with 3 µM GDP (competitive inhibitor) | — | 0.0061 | — | pH 7.8, 60 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphateno ribosome (GTPaseg); with 5 µM GDP (competitive inhibitor) | — | 0.0095 | — | pH 7.8, 60 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphateno ribosome (GTPaseg); with 8 µM GDP (competitive inhibitor) | — | 0.0136 | — | pH 7.8, 60 °C | PMID 9116052 | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |