Values CatLog read from this paper
The site for GTP hydrolysis on the archaeal elongation factor 2 is unmasked by aliphatic alcohols
PMID 9116052doi:10.1016/s0300-9084(97)84335-0Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (27 values) from this paper: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) from Sulfolobus solfataricus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.023 | 9 × 10⁻⁴ | 26 | pH 7.8, 50 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate20-50 pmol SsEF-2 in 200 µL; 0.5-200 µM [gamma-32P]GTP | 0.038 | 0.0012 | 32 | pH 7.8, 60 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.118 | 0.0014 | 84 | pH 7.8, 70 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.152 | 0.001 | 152 | pH 7.8, 75 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.23 | 0.0013 | 177 | pH 7.8, 80 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.512 | 0.0053 | 97 | pH 7.8, 87 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.89 | 0.02 | 45 | pH 7.8, 91 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphate | 0.558 | 0.0532 | 11 | pH 7.8, 95 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphateno ribosome (GTPaseg); with 3 µM GDP (competitive inhibitor) | — | 0.0061 | — | pH 7.8, 60 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphateno ribosome (GTPaseg); with 5 µM GDP (competitive inhibitor) | — | 0.0095 | — | pH 7.8, 60 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |
| elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg) | Sulfolobus solfataricus | guanosine 5'-triphosphateno ribosome (GTPaseg); with 8 µM GDP (competitive inhibitor) | — | 0.0136 | — | pH 7.8, 60 °C | Open record: elongation factor 2 (SsEF-2), intrinsic GTPase (GTPaseg), guanosine 5'-triphosphate |