EC 3.7.1.14: 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase
HydrolasesOpen in Browse
CatLog read 19 records for EC 3.7.1.14 from 2 papers, for 1 organism. Names in these papers: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase), MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 3 records for EC 3.7.1.14 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.7.1.14 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Catalytic mechanism of C-C hydrolase MhpC from Escherichia coli: kinetic analysis of His263 and Ser110 site-directed mutants | Escherichia coli | 11 |
| Catalytic role for arginine 188 in the C-C hydrolase catalytic mechanism for Escherichia coli MhpC and Burkholderia xenovorans LB400 BphD | Escherichia coli | 8 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 28 | 0.0068 | 4.1 × 10³ | pH 8, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase), 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 21 | — | — | pH 7, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase), 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant D235A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.016 | — | — | pH 8, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant D235A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant H114A | Escherichia coli | 2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid | 0.036 | 0.0238 | 1.5 calc. | pH 8, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant H114A, 2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant H114A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 4.5 | 0.0025 | 1.8 × 10³ | pH 8, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant H114A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant H263A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.0029 | 0.0055 | 0.53 | pH 8, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant H263A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant H263A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.0055 | — | — | pH 7, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant H263A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant S110A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.0054 | 0.0185 | 0.29 | pH 8, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant S110A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant S110A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.0035 | — | — | pH 7, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant S110A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant S110G | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.001 | 0.0175 | 0.056 | pH 8, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant S110G, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant S40A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 2.9 | 0.0012 | 2.4 × 10³ | pH 8, 25 °C | PMID 15663941 | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant S40A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant C261A | Escherichia coli | 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid | 12 | 0.0042 | 2.9 × 10³ | pH 8, 25 °C | PMID 17029402 | Open record: MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant C261A, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant F173D | Escherichia coli | 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid | 0.26 | 0.01 | 26 | pH 8, 25 °C | PMID 17029402 | Open record: MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant F173D, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant F173G | Escherichia coli | 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid | 7.5 | 0.058 | 130 | pH 8, 25 °C | PMID 17029402 | Open record: MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant F173G, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant N109A | Escherichia coli | 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid | 0.13 | 0.004 | 33 | pH 8, 25 °C | PMID 17029402 | Open record: MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant N109A, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant N109H | Escherichia coli | 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid | 0.08 | 0.004 | 19 | pH 8, 25 °C | PMID 17029402 | Open record: MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant N109H, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant R188K | Escherichia coli | 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid | 0.74 | 0.038 | 19 | pH 8, 25 °C | PMID 17029402 | Open record: MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant R188K, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant R188Q | Escherichia coli | 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid | 0.1 | 0.077 | 1.4 | pH 8, 25 °C | PMID 17029402 | Open record: MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant R188Q, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant W264G | Escherichia coli | 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid | 2.7 | 0.125 | 21 | pH 8, 25 °C | PMID 17029402 | Open record: MhpC (2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant W264G, 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid |