Values CatLog read from this paper
Catalytic mechanism of C-C hydrolase MhpC from Escherichia coli: kinetic analysis of His263 and Ser110 site-directed mutants
PMID 15663941doi:10.1016/j.jmb.2004.11.032Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (24 values) from this paper: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 28 | 0.0068 | 4.1 × 10³ | pH 8, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase), 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 21 | — | — | pH 7, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase), 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant D235A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.016 | — | — | pH 8, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant D235A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant H114A | Escherichia coli | 2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid | 0.036 | 0.0238 | 1.5 calc. | pH 8, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant H114A, 2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant H114A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 4.5 | 0.0025 | 1.8 × 10³ | pH 8, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant H114A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant H263A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.0029 | 0.0055 | 0.53 | pH 8, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant H263A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant H263A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.0055 | — | — | pH 7, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant H263A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant S110A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.0054 | 0.0185 | 0.29 | pH 8, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant S110A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant S110A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.0035 | — | — | pH 7, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant S110A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant S110G | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 0.001 | 0.0175 | 0.056 | pH 8, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant S110G, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |
| MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase)variant S40A | Escherichia coli | 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid | 2.9 | 0.0012 | 2.4 × 10³ | pH 8, 25 °C | Open record: MhpC (2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase) variant S40A, 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid |