CatLogenzyme kinetics catalog

EC 3.7.1.8: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase

HydrolasesOpen in Browse

CatLog read 22 records for EC 3.7.1.8 from 4 papers, for 3 organisms. Names in these papers: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD), 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (HsaD), BphD (2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid 5,6-hydrolase).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 58 records for EC 3.7.1.8 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.7.1.8 in Browse.

Papers

PaperOrganismsRecords
Identification of a serine hydrolase as a key determinant in the microbial degradation of polychlorinated biphenylsBurkholderia cepacia9
Purification and preliminary characterization of a serine hydrolase involved in the microbial degradation of polychlorinated biphenylsBurkholderia cepacia8
Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolismMycobacterium tuberculosis3
Catalytic role for arginine 188 in the C-C hydrolase catalytic mechanism for Escherichia coli MhpC and Burkholderia xenovorans LB400 BphDBurkholderia xenovorans2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD)Burkholderia cepacia2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoateTable III4.181.9 × 10⁻⁴2.25 × 10⁴pH 7.5, 25 °CPMID 10821847Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD)Burkholderia cepacia2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoateFigure 4—2.1 × 10⁻⁴—pH 7.5, 25 °CPMID 10821847Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD)Burkholderia cepacia3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate0.00895.4 × 10⁻⁴16pH 7.5, 25 °CPMID 10821847Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD)Burkholderia cepacia4-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate5.9 × 10⁻⁴——pH 7.5, 25 °CPMID 10821847Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD)Burkholderia cepacia4-OH HOPDA0.0055——pH 7.5, 25 °CPMID 10821847Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD)Burkholderia cepacia5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate1.530.0049311pH 7.5, 25 °CPMID 10821847Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD)Burkholderia cepacia6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate2.13.3 × 10⁻⁴6.4 × 10³pH 7.5, 25 °CPMID 10821847Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD)Burkholderia cepacia6-(3-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate3.94.6 × 10⁻⁴8.5 × 10³pH 7.5, 25 °CPMID 10821847Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD)Burkholderia cepacia6-(4-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate3.131.3 × 10⁻⁴2.46 × 10⁴pH 7.5, 25 °CPMID 10821847Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD)Burkholderia cepacia2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoateMichaelis-Menten fit (LEONORA)52.5 × 10⁻⁴2 × 10⁴pH 7.5, 25 °CPMID 9722515Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD)Burkholderia cepacia2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoateactivity followed by product (benzoate) formation at 225 nm2.43.9 × 10⁻⁴6.2 × 10³ calc.pH 7.5, 25 °CPMID 9722515Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD)Burkholderia cepacia2-hydroxy-6-oxohexa-2,4-dienoate0.10.0392.6pH 7.5, 25 °CPMID 9722515Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD)Burkholderia cepacia5-methyl-HODA0.0170.310.054pH 7.5, 25 °CPMID 9722515Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD)Burkholderia cepacia6-ethyl-HODA6.70.00411.6 × 10³pH 7.5, 25 °CPMID 9722515Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD)Burkholderia cepacia6-isopropyl-HODA4.80.00133.6 × 10³pH 7.5, 25 °CPMID 9722515Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD)Burkholderia cepacia6-methyl-HODA0.30.0358.6pH 7.5, 25 °CPMID 9722515Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD)Burkholderia cepacia6-tert-butyl-HODA7.50.00145.6 × 10³pH 7.5, 25 °CPMID 9722515Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (HsaD)Mycobacterium tuberculosis2-hydroxy-5-methyl-8-(7a-methyl-1,5-dioxooctahydro-1H-inden-4-yl)-6-oxoocta-2,4-dienoic acid0.550.01733pH 7.5, 25 °CPMID 19875455Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (HsaD)Mycobacterium tuberculosis2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid0.0660.0089pH 7.5, 25 °CPMID 19875455Open
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (HsaD)Mycobacterium tuberculosis8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid0.330.311.06pH 7.5, 25 °CPMID 19875455Open
BphD (2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid 5,6-hydrolase)variant R190KBurkholderia xenovorans2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid0.0090.0022.8pH 8, 25 °CPMID 17029402Open
BphD (2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid 5,6-hydrolase)variant R190QBurkholderia xenovorans2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid0.0160.02820.57pH 8, 25 °CPMID 17029402Open