Values CatLog read from this paper
Purification and preliminary characterization of a serine hydrolase involved in the microbial degradation of polychlorinated biphenyls
PMID 9722515doi:10.1074/jbc.273.36.22943Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (38 values) from this paper: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), HODA hydrolase TodF from Burkholderia cepacia, Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD) | Burkholderia cepacia | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoateMichaelis-Menten fit (LEONORA) | 5 | 2.5 × 10⁻⁴ | 2 × 10⁴ | pH 7.5, 25 °C | Open record: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate |
| 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD) | Burkholderia cepacia | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoateactivity followed by product (benzoate) formation at 225 nm | 2.4 | 3.9 × 10⁻⁴ | 6.2 × 10³ calc. | pH 7.5, 25 °C | Open record: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate |
| 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD) | Burkholderia cepacia | 2-hydroxy-6-oxohexa-2,4-dienoate | 0.1 | 0.039 | 2.6 | pH 7.5, 25 °C | Open record: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), 2-hydroxy-6-oxohexa-2,4-dienoate |
| 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD) | Burkholderia cepacia | 5-methyl-HODA | 0.017 | 0.31 | 0.054 | pH 7.5, 25 °C | Open record: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), 5-methyl-HODA |
| 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD) | Burkholderia cepacia | 6-ethyl-HODA | 6.7 | 0.0041 | 1.6 × 10³ | pH 7.5, 25 °C | Open record: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), 6-ethyl-HODA |
| 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD) | Burkholderia cepacia | 6-isopropyl-HODA | 4.8 | 0.0013 | 3.6 × 10³ | pH 7.5, 25 °C | Open record: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), 6-isopropyl-HODA |
| 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD) | Burkholderia cepacia | 6-methyl-HODA | 0.3 | 0.035 | 8.6 | pH 7.5, 25 °C | Open record: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), 6-methyl-HODA |
| 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD) | Burkholderia cepacia | 6-tert-butyl-HODA | 7.5 | 0.0014 | 5.6 × 10³ | pH 7.5, 25 °C | Open record: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (BphD), 6-tert-butyl-HODA |
| HODA hydrolase TodF | Pseudomonas putida | 2-hydroxy-6-oxohexa-2,4-dienoate | 2.2 | 0.018 | 120 | pH 7.5, 25 °C | Open record: HODA hydrolase TodF, 2-hydroxy-6-oxohexa-2,4-dienoate |
| HODA hydrolase TodF | Pseudomonas putida | 5-methyl-HODA | 2.1 | 0.064 | 30 | pH 7.5, 25 °C | Open record: HODA hydrolase TodF, 5-methyl-HODA |
| HODA hydrolase TodF | Pseudomonas putida | 6-ethyl-HODA | 14 | 0.0015 | 9 × 10³ | pH 7.5, 25 °C | Open record: HODA hydrolase TodF, 6-ethyl-HODA |
| HODA hydrolase TodF | Pseudomonas putida | 6-isopropyl-HODA | 0.41 | 0.022 | 19 | pH 7.5, 25 °C | Open record: HODA hydrolase TodF, 6-isopropyl-HODA |
| HODA hydrolase TodF | Pseudomonas putida | 6-methyl-HODA | 35 | 0.017 | 2 × 10³ | pH 7.5, 25 °C | Open record: HODA hydrolase TodF, 6-methyl-HODA |