CatLogenzyme kinetics catalog

EC 4.1.3.7: citrate (si)-synthase

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CatLog read 60 records for EC 4.1.3.7 from 5 papers, for 8 organisms. Names in these papers: citrate synthase.

EC 4.1.3.7 is no longer current; BRENDA: “transferred to EC 2.3.3.1”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

PaperOrganismsRecords
The kinetic properties of citrate synthase from rat liver mitochondriaRattus norvegicus20
Chimeric allosteric citrate synthases: construction and properties of citrate synthases containing domains from two different enzymesAcinetobacter anitratum, Acinetobacter anitratum (large domain) / Escherichia coli (small domain) and 2 more15
Bacterial citrate synthases: purification, molecular weight and kinetic mechanismAcinetobacter anitratum, Azotobacter vinelandii and 1 more14
Mutation of essential catalytic residues in pig citrate synthaseSus scrofa9
The effects of calcium ions and adenine nucleotides on the activity of pig heart 2-oxoglutarate dehydrogenase complexRattus norvegicus2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
citrate synthaseAcinetobacter anitratumacetyl-coenzyme A412-nm DTNB assay; initial velocity studies; no KCl—0.1—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseAcinetobacter anitratumacetyl-coenzyme A412-nm DTNB assay; initial velocity studies; with 100 mM KCl—0.03—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseAcinetobacter anitratumacetyl-coenzyme A942——pH 7.8, 30 °CPMID 4211224Open
citrate synthaseAcinetobacter anitratumoxaloacetateno KCl added—0.0105—21 °CPMID 1524432Open
citrate synthaseAcinetobacter anitratumoxaloacetate0.1 M KCl—0.0072—21 °CPMID 1524432Open
citrate synthaseAcinetobacter anitratumoxaloacetateno KCl—0.1—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseAcinetobacter anitratumoxaloacetatewith 100 mM KCl—0.1—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseAcinetobacter anitratumS-acetyl-coenzyme Ano KCl added—0.11—21 °CPMID 1524432Open
citrate synthaseAcinetobacter anitratumS-acetyl-coenzyme A0.1 M KCl—0.035—21 °CPMID 1524432Open
citrate synthasechimera CS ACI::ecoAcinetobacter anitratum (large domain) / Escherichia coli (small domain)oxaloacetateno KCl added—0.0132—21 °CPMID 1524432Open
citrate synthasechimera CS ACI::ecoAcinetobacter anitratum (large domain) / Escherichia coli (small domain)oxaloacetate0.1 M KCl—0.0124—21 °CPMID 1524432Open
citrate synthasechimera CS ACI::ecoAcinetobacter anitratum (large domain) / Escherichia coli (small domain)S-acetyl-coenzyme Ano KCl added—0.35—21 °CPMID 1524432Open
citrate synthasechimera CS ACI::ecoAcinetobacter anitratum (large domain) / Escherichia coli (small domain)S-acetyl-coenzyme A0.1 M KCl—0.074—21 °CPMID 1524432Open
citrate synthaseAzotobacter vinelandiiacetyl-coenzyme A412-nm DTNB assay; initial velocity studies; no KCl—0.08—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseAzotobacter vinelandiiacetyl-coenzyme A412-nm DTNB assay; initial velocity studies; with 100 mM KCl—0.03—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseAzotobacter vinelandiiacetyl-coenzyme A2.1 × 10³——pH 7.8, 30 °CPMID 4211224Open
citrate synthaseAzotobacter vinelandiioxaloacetateno KCl—0.02—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseAzotobacter vinelandiioxaloacetatewith 100 mM KCl—0.01—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseBacillus subtilisacetyl-coenzyme Ano KCl—0.06—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseBacillus subtilisacetyl-coenzyme Awith 100 mM KCl—0.04—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseBacillus subtilisoxaloacetateno KCl—0.03—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseBacillus subtilisoxaloacetatewith 100 mM KCl—0.03—pH 7.8, 30 °CPMID 4211224Open
citrate synthaseEscherichia colioxaloacetateno KCl added; [acetyl-CoA] = 1.0 mM—0.021—21 °CPMID 1524432Open
citrate synthaseEscherichia colioxaloacetate0.1 M KCl—0.026—21 °CPMID 1524432Open
citrate synthaseEscherichia coliS-acetyl-coenzyme A—0.12—21 °CPMID 1524432Open
citrate synthasechimera CS ECO::aciEscherichia coli (large domain) / Acinetobacter anitratum (small domain)oxaloacetateno KCl added—0.0094—21 °CPMID 1524432Open
citrate synthasechimera CS ECO::aciEscherichia coli (large domain) / Acinetobacter anitratum (small domain)oxaloacetate0.1 M KCl—0.0077—21 °CPMID 1524432Open
citrate synthasechimera CS ECO::aciEscherichia coli (large domain) / Acinetobacter anitratum (small domain)S-acetyl-coenzyme Ano KCl added—0.36—21 °CPMID 1524432Open
citrate synthasechimera CS ECO::aciEscherichia coli (large domain) / Acinetobacter anitratum (small domain)S-acetyl-coenzyme A0.1 M KCl—0.03—21 °CPMID 1524432Open
citrate synthaseRattus norvegicusacetyl-CoAoxaloacetate fixed at 1.3 µM; apparent Km; Fig. 1—0.0162—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusacetyl-CoAoxaloacetate fixed at 2.5 µM; apparent Km; Fig. 1—0.0169—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusacetyl-CoAoxaloacetate fixed at 3.5 µM; apparent Km; Fig. 1—0.0149—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusacetyl-CoAoxaloacetate fixed at 5.6 µM; apparent Km; Fig. 1—0.0157—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusacetyl-CoAoxaloacetate fixed at 10.6 µM; apparent Km; Fig. 1—0.0166—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusacetyl-CoAFig. 3—0.0163—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusacetyl-CoAoxaloacetate fixed at 34 µM; apparent Km; Fig. 6—0.0172—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusacetyl-CoAoxaloacetate fixed at 34 µM; apparent Km; Fig. 6—0.0275—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusacetyl-CoAoxaloacetate fixed at 34 µM; apparent Km; Fig. 6—0.0656—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusacetyl-CoAoxaloacetate fixed at 34 µM; apparent Km; Fig. 6—0.143—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetatewithout Ca2+ (compared with 30 µM Ca2+)—0.01—pH 7, 30 °CPMID 39549Open
citrate synthaseRattus norvegicusoxaloacetate30 µM Ca2+—0.005—pH 7, 30 °CPMID 39549Open
citrate synthaseRattus norvegicusoxaloacetateacetyl-CoA fixed at 3.5 µM; apparent Km; Fig. 2—0.00203—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetateacetyl-CoA fixed at 7.0 µM; apparent Km; Fig. 2—0.00187—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetateacetyl-CoA fixed at 14.0 µM; apparent Km; Fig. 2—0.0019—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetateacetyl-CoA fixed at 27.5 µM; apparent Km; Fig. 2—0.00196—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetateacetyl-CoA fixed at 55 µM; apparent Km; Fig. 2—0.00196—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetateFig. 4—0.00199—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetateacetyl-CoA fixed at 84 µM; apparent Km; Fig. 7—0.002—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetateacetyl-CoA fixed at 84 µM; apparent Km; Fig. 7—0.0035—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetateacetyl-CoA fixed at 84 µM; apparent Km; Fig. 7—0.0048—25 °CPMID 5820645Open
citrate synthaseRattus norvegicusoxaloacetateacetyl-CoA fixed at 84 µM; apparent Km; Fig. 7—0.0048—25 °CPMID 5820645Open
citrate synthaseSus scrofaacetyl coenzyme A—0.0163—pH 8.1, 25 °CPMID 1702991Open
citrate synthaseSus scrofaoxaloacetate960.0061.6 × 10⁴ calc.pH 8.1, 25 °CPMID 1702991Open
citrate synthasevariant D375ESus scrofaacetyl coenzyme A—0.0041—pH 8.1, 25 °CPMID 1702991Open
citrate synthasevariant D375ESus scrofaoxaloacetate0.236 × 10⁻⁴380 calc.pH 8.1, 25 °CPMID 1702991Open
citrate synthasevariant D375GSus scrofaoxaloacetate0.06——pH 8.1, 25 °CPMID 1702991Open
citrate synthasevariant H274GSus scrofaacetyl coenzyme A—0.0054—pH 8.1, 25 °CPMID 1702991Open
citrate synthasevariant H274GSus scrofaoxaloacetate0.10.003529 calc.pH 8.1, 25 °CPMID 1702991Open
citrate synthasevariant H274RSus scrofaacetyl coenzyme A—0.0065—pH 8.1, 25 °CPMID 1702991Open
citrate synthasevariant H274RSus scrofaoxaloacetate0.150.003839 calc.pH 8.1, 25 °CPMID 1702991Open