CatLogenzyme kinetics catalog

Values CatLog read from this paper

The effects of calcium ions and adenine nucleotides on the activity of pig heart 2-oxoglutarate dehydrogenase complex

PMID 39549doi:10.1042/bj1800533Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (20 values) from this paper: 2-oxoglutarate dehydrogenase complex, citrate synthase from Sus scrofa, Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.1.3.7

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutaratecalculated free Ca2+ <10^-9 M—2.5—pH 7, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutaratecalculated free Ca2+ 3.14 x 10^-7 M—0.98—pH 7, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutaratecalculated free Ca2+ 5.01 x 10^-7 M—0.65—pH 7, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutaratecalculated free Ca2+ 1.93 x 10^-6 M—0.31—pH 7, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutaratecalculated free Ca2+ 3.27 x 10^-5 M—0.16—pH 7, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutarate1 mM NAD+; 1 mM thiamin pyrophosphate; 0.25 mM CoA—1.2—pH 6.8, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutarate1 mM NAD+; 1 mM thiamin pyrophosphate; 0.25 mM CoA—0.15—pH 6.8, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutarate—0.2—30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutaratesaturating Ca2+ (separate experiment)—0.08—pH 6.8, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutaratesaturating Ca2+ (separate experiment); in the text—0.3—pH 7.2, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutarate1 mM NAD+; 1 mM thiamin pyrophosphate; 0.25 mM CoA; Table 4—24.5—pH 7.2, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutarate1 mM NAD+; 1 mM thiamin pyrophosphate; 0.25 mM CoA; Table 4—8.6—pH 7.2, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutarate1 mM NAD+; 1 mM thiamin pyrophosphate; 0.25 mM CoA; Table 4—0.84—pH 7.2, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutarate1 mM NAD+; 1 mM thiamin pyrophosphate; 0.25 mM CoA; Table 4—0.79—pH 7.2, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutarate1 mM NAD+; 1 mM thiamin pyrophosphate; 0.25 mM CoA; Table 4—0.34—pH 7.2, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofa2-oxoglutarate1 mM NAD+; 1 mM thiamin pyrophosphate; 0.25 mM CoA; Table 4—0.075—pH 7.2, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofaNAD+free Ca2+ <1 nM—0.0612—pH 7, 30 °COpen
2-oxoglutarate dehydrogenase complexSus scrofaNAD+free Ca2+ 33 µM—0.0619—pH 7, 30 °COpen
citrate synthaseRattus norvegicusoxaloacetatewithout Ca2+ (compared with 30 µM Ca2+)—0.01—pH 7, 30 °COpen
citrate synthaseRattus norvegicusoxaloacetate30 µM Ca2+—0.005—pH 7, 30 °COpen