CatLogenzyme kinetics catalog

EC 4.2.1.22: cystathionine beta-synthase

LyasesOpen in Browse

CatLog read 90 records for EC 4.2.1.22 from 7 papers, for 5 organisms. Names in these papers: cystathionine beta-synthase (ytCBS-His), cystathionine β-synthase (CBS-A), cystathionine beta-synthase, cystathionine beta-synthase (BaCBS) and 2 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 46 records for EC 4.2.1.22 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.2.1.22 in Browse.

Papers

PaperOrganismsRecords
Role of active-site residues Thr81, Ser82, Thr85, Gln157, and Tyr158 in yeast cystathionine beta-synthase catalysis and reaction specificity—31
Crystallographic and mutational analyses of cystathionine β-synthase in the H2 S-synthetic gene cluster in Lactobacillus plantarumLactobacillus plantarum22
Biogenesis of Hydrogen Sulfide and Thioethers by Cystathionine Beta-SynthaseHomo sapiens10
Trypsin cleavage of human cystathionine beta-synthase into an evolutionarily conserved active core: structural and functional consequencesHomo sapiens10
Structural characterization and functional analysis of cystathionine β-synthase: an enzyme involved in the reverse transsulfuration pathway of Bacillus anthracisBacillus anthracis9
Characterization of the S289A,D mutants of yeast cystathionine beta-synthaseyeast4
Insights into Domain Organization and Regulatory Mechanism of Cystathionine Beta-Synthase from Toxoplasma gondiiToxoplasma gondii4

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
cystathionine beta-synthaseHomo sapienshydrogen sulfideno AdoMet0.355.020.07pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthaseHomo sapienshydrogen sulfidewith 0.5 mM AdoMet0.784.70.17pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthaseHomo sapiensL-cysteineL-Cys varied; L-Hcy fixed at 20 mM (saturating); no AdoMet7.0913.410.53pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthaseHomo sapiensL-cysteineL-Cys varied; L-Hcy fixed at 20 mM (saturating)31.3415.921.98pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthaseHomo sapiensL-cysteineno AdoMet1.068.110.13pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthaseHomo sapiensL-cysteinewith 0.5 mM AdoMet1.418.410.18pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthaseHomo sapiensL-homocysteinewithout AdoMet61.53.9pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-homocysteinewith AdoMet (S-adenosyl-L-methionine) as activator20.51.612.7pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-homocysteinedimer6.70.512.5pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-homocysteineAdoMet presence not stated in Table III—1.54—pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-homocysteineAdoMet presence not stated in Table III; dimer—0.53—pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-homocysteineno AdoMet8.174.311.93pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthaseHomo sapiensL-homocysteinewith 0.5 mM AdoMet26.784.486.02pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthaseHomo sapiensL-serinewithout AdoMet632pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-serinewith AdoMet (S-adenosyl-L-methionine) as activator20.52.48.6pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-serinedimer6.72.72.6pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-serineAdoMet presence not stated in Table III—2.39—pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-serineAdoMet presence not stated in Table III; dimer—2.7—pH 8.6, 37 °CPMID 9675031Open
cystathionine beta-synthaseHomo sapiensL-serineno AdoMet0.391.270.28pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthaseHomo sapiensL-serinewith 0.5 mM AdoMet0.621.320.47pH 8.6, 37 °CPMID 28874062Open
cystathionine beta-synthase (BaCBS)Bacillus anthracisHomocysteineOAS-dependent CBS assay: 50 mM Tris-HCl pH 8.2; 0.25 mM PLP8.392.733.06pH 8.2, 37 °CPMID 28921884Open
cystathionine beta-synthase (BaCBS)Bacillus anthracisHomocysteine0.4 mM lead acetate; cysteine held at 250 µM; 0.73 µM enzyme3.380.3210.56pH 8.2, 37 °CPMID 28921884Open
cystathionine beta-synthase (BaCBS)Bacillus anthracisO-acetylserine4.461.1683.82pH 8.2, 37 °CPMID 28921884Open
cystathionine beta-synthase (BaCBS)Bacillus anthracisO-acetylserine0.76.180.11pH 7.4, 25 °CPMID 28921884Open
cystathionine beta-synthase (BaCBS)variant A72SBacillus anthracisHomocysteine10.913.593.04pH 8.2, 37 °CPMID 28921884Open
cystathionine beta-synthase (BaCBS)variant A72SBacillus anthracisO-acetylserine0.99.010.1pH 7.4, 25 °CPMID 28921884Open
cystathionine beta-synthase (BaCBS)variant A72SBacillus anthracisO-acetylserine4.881.652.96pH 8.2, 37 °CPMID 28921884Open
cystathionine beta-synthase (BaCBS)variant E220RBacillus anthracisHomocysteine5.741.513.83pH 8.2, 37 °CPMID 28921884Open
cystathionine beta-synthase (BaCBS)variant E220RBacillus anthracisO-acetylserine5.462.052.67pH 8.2, 37 °CPMID 28921884Open
cystathionine beta-synthase (TgCBS)variant R353*Toxoplasma gondiiL-homocysteineL-Ser + L-Hcys -> L-Cth; 0.1-30 mM L-Ser; cosubstrate L-Ser—0.229pH 9, 37 °CPMID 35897745Open
cystathionine beta-synthase (TgCBS)variant R353*Toxoplasma gondiiL-homocysteineL-OAS + L-Hcys -> L-Cth; 1-100 mM L-OAS; cosubstrate L-OAS—0.2721pH 9, 37 °CPMID 35897745Open
cystathionine beta-synthase (TgCBS)variant R353*Toxoplasma gondiiL-serine5.70.610pH 9, 37 °CPMID 35897745Open
cystathionine beta-synthase (TgCBS)variant R353*Toxoplasma gondiiO-acetyl-L-serine5.62.82pH 9, 37 °CPMID 35897745Open
cystathionine beta-synthase (ytCBS)variant S289AyeastL-homocysteine—0.166.3pH 8.6, 37 °CPMID 19264153Open
cystathionine beta-synthase (ytCBS)variant S289AyeastL-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth0.8527.10.031pH 8.6, 37 °CPMID 19264153Open
cystathionine beta-synthase (ytCBS)variant S289AyeastL-serinebeta-elimination reaction L-Ser -> pyruvate + NH30.006156.41.1 × 10⁻⁴pH 8.6, 37 °CPMID 19264153Open
cystathionine beta-synthase (ytCBS)variant S289AyeastS-(2-amino-2-carboxyethyl)-L-homocysteine0.00450.90.0053pH 8.6, 37 °CPMID 19264153Open
cystathionine beta-synthase (ytCBS-His)—L-cystathionine1.03—7.5pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)—L-homocysteine——80pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)—L-serine17—25pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Q157A—L-cystathioninereverse reaction; hydrolysis of L-Cth to L-Ser + L-Hcys——0.00118pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Q157A—L-cystathioninebeta-elimination reaction L-Cth -> L-Hcys + pyruvate + NH3——0.00105pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Q157A—L-serine1.24—0.0062pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Q157E—L-cystathioninereverse reaction; hydrolysis of L-Cth to L-Ser + L-Hcys——0.0042pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Q157E—L-cystathioninebeta-elimination reaction L-Cth -> L-Hcys + pyruvate + NH30.0237—0.023pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Q157E—L-serine0.73—0.0019pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Q157H—L-homocysteine——0.7pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Q157H—L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth0.082—0.021pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Q157H—L-serinecoupled assay: continuous LDH assay for pyruvate formation0.075—3.9 × 10⁻⁴pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant S82A—L-cystathionine0.083—0.08pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant S82A—L-homocysteine——20pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant S82A—L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth5.3—0.33pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant S82A—L-serinecoupled assay: continuous LDH assay for pyruvate formation0.0208—0.016pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T81A—L-cystathionine——8 × 10⁻⁴pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T81A—L-homocysteine——2.1pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T81A—L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth0.45—0.0032pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T81A—L-serinecoupled assay: continuous LDH assay for pyruvate formation0.0051—9 × 10⁻⁵pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T85A—L-cystathioninereverse reaction; hydrolysis of L-Cth to L-Ser + L-Hcys0.133—0.54pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T85A—L-cystathioninebeta-elimination reaction L-Cth -> L-Hcys + pyruvate + NH30.035—0.07pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T85A—L-homocysteine——16pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T85A—L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth13.2—6.1pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T85A—L-serinecoupled assay: continuous LDH assay for pyruvate formation0.073—0.56pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant T85A—L-serineby fitting to eq 70.10.50.20 calc.pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Y158F—L-cystathioninereverse reaction; hydrolysis of L-Cth to L-Ser + L-Hcys0.418—1.49pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Y158F—L-cystathioninebeta-elimination reaction L-Cth -> L-Hcys + pyruvate + NH30.0055—0.013pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Y158F—L-homocysteine——30pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Y158F—L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth8.2—7pH 8.6, 37 °CPMID 14967036Open
cystathionine beta-synthase (ytCBS-His)variant Y158F—L-serinecoupled assay: continuous LDH assay for pyruvate formation0.0176—0.09pH 8.6, 37 °CPMID 14967036Open
cystathionine β-synthase (CBS-A)Lactobacillus plantarumL-cysteinesingle-substrate fit (Eq. 4)1.18.90.12pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)Lactobacillus plantarumL-cysteine0.1-40 mM L-homocysteine; cosubstrate L-homocysteine7.65.91.3pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)Lactobacillus plantarumL-homocysteine0.2-100 mM L-OAS; 0.1-20 mM L-homocysteine; 10 min at 37 °C—179pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)Lactobacillus plantarumL-homocysteine1-150 mM L-cysteine; 0.1-40 mM L-homocysteine; 37 °C—0.5414pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)Lactobacillus plantarumO-acetyl-L-serine1 mM tris(2-carboxyethyl)phosphine; 0.2-100 mM L-OAS110814pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)Lactobacillus plantarumO-acetyl-L-serine1 mM tris(2-carboxyethyl)phosphine; 0.2-100 mM L-OAS774.219pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)Lactobacillus plantarumO-acetyl-L-serine0.01-1 mM L-OAS; 500 µg/mL CBS; 37 °C; A340 continuous0.0140.0340.43pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)Lactobacillus plantarumsodium sulfide—1.958pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant A70SLactobacillus plantarumL-cysteinesingle-substrate fit (Eq. 4)——0.002pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant A70SLactobacillus plantarumL-cysteine0.1-40 mM L-homocysteine; cosubstrate L-homocysteine231.5pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant A70SLactobacillus plantarumL-homocysteine0.2-100 mM L-OAS; 0.1-20 mM L-homocysteine; 10 min at 37 °C—2.71.3pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant A70SLactobacillus plantarumL-homocysteine1-150 mM L-cysteine; 0.1-40 mM L-homocysteine; 37 °C—200.15pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant A70SLactobacillus plantarumO-acetyl-L-serine0.1-4 mM Na2S; cosubstrate Na2S3.90.755.2pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant A70SLactobacillus plantarumO-acetyl-L-serine0.1-20 mM L-homocysteine; cosubstrate L-homocysteine3.50.477.5pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant A70SLactobacillus plantarumsodium sulfide—1.82.1pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant E223GLactobacillus plantarumL-cysteinesingle-substrate fit (Eq. 4)0.022376 × 10⁻⁴pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant E223GLactobacillus plantarumL-cysteine0.1-40 mM L-homocysteine; cosubstrate L-homocysteine0.33370.0089pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant E223GLactobacillus plantarumL-homocysteine0.2-100 mM L-OAS; 0.1-20 mM L-homocysteine; 10 min at 37 °C—0.269.2pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant E223GLactobacillus plantarumL-homocysteine1-150 mM L-cysteine; 0.1-40 mM L-homocysteine; 37 °C—0.40.83pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant E223GLactobacillus plantarumO-acetyl-L-serine0.1-4 mM Na2S; cosubstrate Na2S5280.18pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant E223GLactobacillus plantarumO-acetyl-L-serine0.1-20 mM L-homocysteine; cosubstrate L-homocysteine2.4190.12pH 7.5, 37 °CPMID 28127810Open
cystathionine β-synthase (CBS-A)variant E223GLactobacillus plantarumsodium sulfide—0.2818pH 7.5, 37 °CPMID 28127810Open