Values CatLog read from this paper
Role of active-site residues Thr81, Ser82, Thr85, Gln157, and Tyr158 in yeast cystathionine beta-synthase catalysis and reaction specificity
PMID 14967036doi:10.1021/bi035496mOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 31 records (52 values) from this paper: cystathionine beta-synthase (ytCBS-His).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cystathionine beta-synthase (ytCBS-His) | — | L-cystathionine | 1.03 | — | 7.5 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His), L-cystathionine |
| cystathionine beta-synthase (ytCBS-His) | — | L-homocysteine | — | — | 80 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His), L-homocysteine |
| cystathionine beta-synthase (ytCBS-His) | — | L-serine | 17 | — | 25 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His), L-serine |
| cystathionine beta-synthase (ytCBS-His)variant Q157A | — | L-cystathioninereverse reaction; hydrolysis of L-Cth to L-Ser + L-Hcys | — | — | 0.00118 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Q157A, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant Q157A | — | L-cystathioninebeta-elimination reaction L-Cth -> L-Hcys + pyruvate + NH3 | — | — | 0.00105 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Q157A, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant Q157A | — | L-serine | 1.24 | — | 0.0062 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Q157A, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant Q157E | — | L-cystathioninereverse reaction; hydrolysis of L-Cth to L-Ser + L-Hcys | — | — | 0.0042 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Q157E, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant Q157E | — | L-cystathioninebeta-elimination reaction L-Cth -> L-Hcys + pyruvate + NH3 | 0.0237 | — | 0.023 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Q157E, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant Q157E | — | L-serine | 0.73 | — | 0.0019 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Q157E, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant Q157H | — | L-homocysteine | — | — | 0.7 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Q157H, L-homocysteine |
| cystathionine beta-synthase (ytCBS-His)variant Q157H | — | L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth | 0.082 | — | 0.021 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Q157H, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant Q157H | — | L-serinecoupled assay: continuous LDH assay for pyruvate formation | 0.075 | — | 3.9 × 10⁻⁴ | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Q157H, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant S82A | — | L-cystathionine | 0.083 | — | 0.08 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant S82A, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant S82A | — | L-homocysteine | — | — | 20 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant S82A, L-homocysteine |
| cystathionine beta-synthase (ytCBS-His)variant S82A | — | L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth | 5.3 | — | 0.33 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant S82A, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant S82A | — | L-serinecoupled assay: continuous LDH assay for pyruvate formation | 0.0208 | — | 0.016 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant S82A, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant T81A | — | L-cystathionine | — | — | 8 × 10⁻⁴ | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T81A, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant T81A | — | L-homocysteine | — | — | 2.1 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T81A, L-homocysteine |
| cystathionine beta-synthase (ytCBS-His)variant T81A | — | L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth | 0.45 | — | 0.0032 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T81A, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant T81A | — | L-serinecoupled assay: continuous LDH assay for pyruvate formation | 0.0051 | — | 9 × 10⁻⁵ | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T81A, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant T85A | — | L-cystathioninereverse reaction; hydrolysis of L-Cth to L-Ser + L-Hcys | 0.133 | — | 0.54 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T85A, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant T85A | — | L-cystathioninebeta-elimination reaction L-Cth -> L-Hcys + pyruvate + NH3 | 0.035 | — | 0.07 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T85A, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant T85A | — | L-homocysteine | — | — | 16 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T85A, L-homocysteine |
| cystathionine beta-synthase (ytCBS-His)variant T85A | — | L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth | 13.2 | — | 6.1 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T85A, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant T85A | — | L-serinecoupled assay: continuous LDH assay for pyruvate formation | 0.073 | — | 0.56 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T85A, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant T85A | — | L-serineby fitting to eq 7 | 0.1 | 0.5 | 0.20 calc. | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant T85A, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant Y158F | — | L-cystathioninereverse reaction; hydrolysis of L-Cth to L-Ser + L-Hcys | 0.418 | — | 1.49 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Y158F, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant Y158F | — | L-cystathioninebeta-elimination reaction L-Cth -> L-Hcys + pyruvate + NH3 | 0.0055 | — | 0.013 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Y158F, L-cystathionine |
| cystathionine beta-synthase (ytCBS-His)variant Y158F | — | L-homocysteine | — | — | 30 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Y158F, L-homocysteine |
| cystathionine beta-synthase (ytCBS-His)variant Y158F | — | L-serinebeta-replacement reaction L-Ser + L-Hcys -> L-Cth | 8.2 | — | 7 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Y158F, L-serine |
| cystathionine beta-synthase (ytCBS-His)variant Y158F | — | L-serinecoupled assay: continuous LDH assay for pyruvate formation | 0.0176 | — | 0.09 | pH 8.6, 37 °C | Open record: cystathionine beta-synthase (ytCBS-His) variant Y158F, L-serine |