EC 4.2.1.31: maleate hydratase

LyasesOpen in Browse

CatLog read 7 records for EC 4.2.1.31 from 1 paper, for 1 organism. Names in these papers: isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 2 records for EC 4.2.1.31 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.2.1.31 in Browse.

Papers

Papers with records for EC 4.2.1.31
Paper	Organisms	Records
Enzymology and evolution of the pyruvate pathway to 2-oxobutyrate in Methanocaldococcus jannaschii	Methanocaldococcus jannaschii	7

Records

Records for EC 4.2.1.31 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277	Methanocaldococcus jannaschii	(R)-2-hydroxy-2-methylbutanedioate ((R)-citramalate)	1.6	0.81	2	pH 7.5, 60 °C	PMID 17449626	Open record: isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277, (R)-2-hydroxy-2-methylbutanedioate ((R)-citramalate)
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277	Methanocaldococcus jannaschii	2-isopropylmalate (racemic, commercial)	4.5	1.9	2.4	pH 7.5, 60 °C	PMID 17449626	Open record: isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277, 2-isopropylmalate (racemic, commercial)
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277	Methanocaldococcus jannaschii	citraconate (cis-2-methylbutenedioate, methylmaleate)continuous UV absorbance assay of hydrolyase activity	16	0.08	200	pH 7.5, 60 °C	PMID 17449626	Open record: isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277, citraconate (cis-2-methylbutenedioate, methylmaleate)
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277	Methanocaldococcus jannaschii	citraconate (cis-2-methylbutenedioate, methylmaleate)coupled assay: excess IPMDHMj (30 µg/ml) + 5 mM NAD+	14	0.04	350	pH 7.5, 60 °C	PMID 17449626	Open record: isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277, citraconate (cis-2-methylbutenedioate, methylmaleate)
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277	Methanocaldococcus jannaschii	DL-threo-3-isopropylmalate (racemic; physiological isomer (2R,3S)-3-isopropylmalate)	1.9	0.039	50	pH 7.5, 60 °C	PMID 17449626	Open record: isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277, DL-threo-3-isopropylmalate (racemic; physiological isomer (2R,3S)-3-isopropylmalate)
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277	Methanocaldococcus jannaschii	maleate (cis-butenedioate)continuous UV absorbance assay of hydrolyase activity	36	0.4	90	pH 7.5, 60 °C	PMID 17449626	Open record: isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277, maleate (cis-butenedioate)
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277	Methanocaldococcus jannaschii	maleate (cis-butenedioate)coupled assay: excess IPMDHMj (30 µg/ml) + 5 mM NAD+	20	0.18	110	pH 7.5, 60 °C	PMID 17449626	Open record: isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277, maleate (cis-butenedioate)