CatLogenzyme kinetics catalog

Values CatLog read from this paper

Enzymology and evolution of the pyruvate pathway to 2-oxobutyrate in Methanocaldococcus jannaschii

PMID 17449626doi:10.1128/jb.00166-07Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 9 records (25 values) from this paper: isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277, isopropylmalate dehydrogenase (IPMDHMj, MJ0720) from Methanocaldococcus jannaschii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.n5, EC 1.1.1.83, EC 1.1.1.85, EC 4.2.1.31, EC 4.2.1.33, EC 4.2.1.35

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
isopropylmalate dehydrogenase (IPMDHMj, MJ0720)Methanocaldococcus jannaschii(R)-malate—0.4114pH 8.5, 60 °COpen
isopropylmalate dehydrogenase (IPMDHMj, MJ0720)Methanocaldococcus jannaschiiDL-threo-3-isopropylmalate (racemic; physiological isomer (2R,3S)-3-isopropylmalate)—0.02488pH 8.5, 60 °COpen
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277Methanocaldococcus jannaschii(R)-2-hydroxy-2-methylbutanedioate ((R)-citramalate)1.60.812pH 7.5, 60 °COpen
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277Methanocaldococcus jannaschii2-isopropylmalate (racemic, commercial)4.51.92.4pH 7.5, 60 °COpen
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277Methanocaldococcus jannaschiicitraconate (cis-2-methylbutenedioate, methylmaleate)continuous UV absorbance assay of hydrolyase activity160.08200pH 7.5, 60 °COpen
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277Methanocaldococcus jannaschiicitraconate (cis-2-methylbutenedioate, methylmaleate)coupled assay: excess IPMDHMj (30 µg/ml) + 5 mM NAD+140.04350pH 7.5, 60 °COpen
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277Methanocaldococcus jannaschiiDL-threo-3-isopropylmalate (racemic; physiological isomer (2R,3S)-3-isopropylmalate)1.90.03950pH 7.5, 60 °COpen
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277Methanocaldococcus jannaschiimaleate (cis-butenedioate)continuous UV absorbance assay of hydrolyase activity360.490pH 7.5, 60 °COpen
isopropylmalate isomerase (citramalate/isopropylmalate isomerase) MJ0499/MJ1277Methanocaldococcus jannaschiimaleate (cis-butenedioate)coupled assay: excess IPMDHMj (30 µg/ml) + 5 mM NAD+200.18110pH 7.5, 60 °COpen