EC 4.2.1.96: 4a-hydroxytetrahydrobiopterin dehydratase
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CatLog read 20 records for EC 4.2.1.96 from 2 papers, for 1 organism. Names in these papers: phenylalanine hydroxylase (PheH), phenylalanine hydroxylase (Δ117PheH).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 26 records for EC 4.2.1.96 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.2.1.96 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Reversing the substrate specificities of phenylalanine and tyrosine hydroxylase: aspartate 425 of tyrosine hydroxylase is essential for L-DOPA formation | Rattus norvegicus | 12 |
| Insights into the catalytic mechanisms of phenylalanine and tryptophan hydroxylase from kinetic isotope effects on aromatic hydroxylation | Rattus norvegicus | 8 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| phenylalanine hydroxylase (PheH) | Rattus norvegicus | L-phenylalanine | 16 | 0.252 | 63 | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH), L-phenylalanine |
| phenylalanine hydroxylase (PheH) | Rattus norvegicus | L-tyrosine | 0.0013 | — | 6 × 10⁻⁴ | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH), L-tyrosine |
| phenylalanine hydroxylase (PheH)variant H264Q | Rattus norvegicus | L-phenylalanine | 14 | 0.569 | 24.7 | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant H264Q, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant H264Q | Rattus norvegicus | L-tyrosine | 8.3 × 10⁻⁴ | — | 3.8 × 10⁻⁴ | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant H264Q, L-tyrosine |
| phenylalanine hydroxylase (PheH)variant H264Q/V379D | Rattus norvegicus | L-phenylalanine | 4.68 | 1.08 | 4.3 | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant H264Q/V379D, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant H264Q/V379D | Rattus norvegicus | L-tyrosine | 0.02 | 0.146 | 0.137 | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant H264Q/V379D, L-tyrosine |
| phenylalanine hydroxylase (PheH)variant H264Q/Y277H/V379D | Rattus norvegicus | L-phenylalanine | 2.08 | 0.218 | 9.5 | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant H264Q/Y277H/V379D, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant H264Q/Y277H/V379D | Rattus norvegicus | L-tyrosine | 0.0305 | 1.31 | 0.023 | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant H264Q/Y277H/V379D, L-tyrosine |
| phenylalanine hydroxylase (PheH)variant V379D | Rattus norvegicus | L-phenylalanine | 0.63 | 0.191 | 3.3 | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant V379D, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant V379D | Rattus norvegicus | L-tyrosine | 8.3 × 10⁻⁴ | — | 3.8 × 10⁻⁴ | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant V379D, L-tyrosine |
| phenylalanine hydroxylase (PheH)variant Y277H | Rattus norvegicus | L-phenylalanine | 10.07 | 0.574 | 17.5 | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant Y277H, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant Y277H | Rattus norvegicus | L-tyrosine | 0.001 | — | 4.5 × 10⁻⁴ | pH 6.7, 32 °C | PMID 10933781 | Open record: phenylalanine hydroxylase (PheH) variant Y277H, L-tyrosine |
| phenylalanine hydroxylase (Δ117PheH) | Rattus norvegicus | 6-methyltetrahydropterin | — | 0.083 | — | pH 7, 25 °C | PMID 16953590 | Open record: phenylalanine hydroxylase (Δ117PheH), 6-methyltetrahydropterin |
| phenylalanine hydroxylase (Δ117PheH) | Rattus norvegicus | L-phenylalaninetyrosine formation (A275); 5 mM DTT | 8.55 | 0.12 | 72 | pH 7, 25 °C | PMID 16953590 | Open record: phenylalanine hydroxylase (Δ117PheH), L-phenylalanine |
| phenylalanine hydroxylase (Δ117PheH) | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation; 200 µM 6-MePH4 (Table 1 footnote b) | 7.85 | 0.127 | 61.8 calc. | pH 7, 25 °C | PMID 16953590 | Open record: phenylalanine hydroxylase (Δ117PheH), L-phenylalanine |
| phenylalanine hydroxylase (Δ117PheH) | Rattus norvegicus | L-tryptophan200 µM 6-MePH4; 1.5 µM enzyme; 7 mM DTT; 50 µg/mL catalase | 0.55 | 0.7 | 0.79 calc. | pH 7, 25 °C | PMID 16953590 | Open record: phenylalanine hydroxylase (Δ117PheH), L-tryptophan |
| phenylalanine hydroxylase (Δ117PheH) | Rattus norvegicus | L-tryptophan6-MePH4 oxidation with L-tryptophan; 60 µg/mL catalase | 0.78 | 1 | 0.78 calc. | pH 7, 25 °C | PMID 16953590 | Open record: phenylalanine hydroxylase (Δ117PheH), L-tryptophan |
| phenylalanine hydroxylase (Δ117PheH)variant V379D | Rattus norvegicus | 6-methyltetrahydropterin | — | 0.063 | — | pH 7, 25 °C | PMID 16953590 | Open record: phenylalanine hydroxylase (Δ117PheH) variant V379D, 6-methyltetrahydropterin |
| phenylalanine hydroxylase (Δ117PheH)variant V379D | Rattus norvegicus | L-phenylalaninetyrosine formation (A275); 5 mM DTT | 0.3 | 0.42 | 0.72 | pH 7, 25 °C | PMID 16953590 | Open record: phenylalanine hydroxylase (Δ117PheH) variant V379D, L-phenylalanine |
| phenylalanine hydroxylase (Δ117PheH)variant V379D | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation; 200 µM 6-MePH4 (Table 1 footnote b) | 1.12 | 0.625 | 1.80 calc. | pH 7, 25 °C | PMID 16953590 | Open record: phenylalanine hydroxylase (Δ117PheH) variant V379D, L-phenylalanine |