Values CatLog read from this paper
Reversing the substrate specificities of phenylalanine and tyrosine hydroxylase: aspartate 425 of tyrosine hydroxylase is essential for L-DOPA formation
PMID 10933781doi:10.1021/bi000493kOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (56 values) from this paper: phenylalanine hydroxylase (PheH), tyrosine hydroxylase (TyrH) from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 1.14.16.1, EC 1.14.16.2, EC 4.2.1.96
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phenylalanine hydroxylase (PheH) | Rattus norvegicus | L-phenylalanine | 16 | 0.252 | 63 | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH), L-phenylalanine |
| phenylalanine hydroxylase (PheH) | Rattus norvegicus | L-tyrosine | 0.0013 | — | 6 × 10⁻⁴ | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH), L-tyrosine |
| phenylalanine hydroxylase (PheH)variant H264Q | Rattus norvegicus | L-phenylalanine | 14 | 0.569 | 24.7 | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant H264Q, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant H264Q | Rattus norvegicus | L-tyrosine | 8.3 × 10⁻⁴ | — | 3.8 × 10⁻⁴ | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant H264Q, L-tyrosine |
| phenylalanine hydroxylase (PheH)variant H264Q/V379D | Rattus norvegicus | L-phenylalanine | 4.68 | 1.08 | 4.3 | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant H264Q/V379D, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant H264Q/V379D | Rattus norvegicus | L-tyrosine | 0.02 | 0.146 | 0.137 | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant H264Q/V379D, L-tyrosine |
| phenylalanine hydroxylase (PheH)variant H264Q/Y277H/V379D | Rattus norvegicus | L-phenylalanine | 2.08 | 0.218 | 9.5 | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant H264Q/Y277H/V379D, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant H264Q/Y277H/V379D | Rattus norvegicus | L-tyrosine | 0.0305 | 1.31 | 0.023 | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant H264Q/Y277H/V379D, L-tyrosine |
| phenylalanine hydroxylase (PheH)variant V379D | Rattus norvegicus | L-phenylalanine | 0.63 | 0.191 | 3.3 | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant V379D, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant V379D | Rattus norvegicus | L-tyrosine | 8.3 × 10⁻⁴ | — | 3.8 × 10⁻⁴ | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant V379D, L-tyrosine |
| phenylalanine hydroxylase (PheH)variant Y277H | Rattus norvegicus | L-phenylalanine | 10.07 | 0.574 | 17.5 | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant Y277H, L-phenylalanine |
| phenylalanine hydroxylase (PheH)variant Y277H | Rattus norvegicus | L-tyrosine | 0.001 | — | 4.5 × 10⁻⁴ | pH 6.7, 32 °C | Open record: phenylalanine hydroxylase (PheH) variant Y277H, L-tyrosine |
| tyrosine hydroxylase (TyrH) | Rattus norvegicus | L-phenylalanine | 1.6 | 0.1 | 16 | pH 6.7, 32 °C | Open record: tyrosine hydroxylase (TyrH), L-phenylalanine |
| tyrosine hydroxylase (TyrH) | Rattus norvegicus | L-tyrosine | 2.5 | 0.016 | 156.3 | pH 6.7, 32 °C | Open record: tyrosine hydroxylase (TyrH), L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425V | Rattus norvegicus | L-phenylalanine | 4.5 | 0.00125 | 3.75 × 10³ | pH 6.7, 32 °C | Open record: tyrosine hydroxylase (TyrH) variant D425V, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425V | Rattus norvegicus | L-tyrosine | 0.0207 | 0.045 | 0.47 | pH 6.7, 32 °C | Open record: tyrosine hydroxylase (TyrH) variant D425V, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant H323Y | Rattus norvegicus | L-phenylalanine | 7.28 | 0.0077 | 947 | pH 6.7, 32 °C | Open record: tyrosine hydroxylase (TyrH) variant H323Y, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant H323Y | Rattus norvegicus | L-tyrosine | 2.65 | 0.092 | 28.8 | pH 6.7, 32 °C | Open record: tyrosine hydroxylase (TyrH) variant H323Y, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant Q310H | Rattus norvegicus | L-phenylalanine | 2.03 | 0.0088 | 232 | pH 6.7, 32 °C | Open record: tyrosine hydroxylase (TyrH) variant Q310H, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant Q310H | Rattus norvegicus | L-tyrosine | 2.13 | 0.054 | 39.5 | pH 6.7, 32 °C | Open record: tyrosine hydroxylase (TyrH) variant Q310H, L-tyrosine |