EC 1.14.16.2: tyrosine 3-monooxygenase

OxidoreductasesOpen in Browse

CatLog read 85 records for EC 1.14.16.2 from 4 papers, for 2 organisms. Names in these papers: tyrosine hydroxylase, tyrosine hydroxylase (TyrH).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 45 records for EC 1.14.16.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.16.2 in Browse.

Papers

Papers with records for EC 1.14.16.2
Paper	Organisms	Records
Mutation of regulatory serines of rat tyrosine hydroxylase to glutamate: effects on enzyme stability and activity	Rattus norvegicus	43
Mutational analysis of catecholamine binding in tyrosine hydroxylase	Homo sapiens	24
Mutation to phenylalanine of tyrosine 371 in tyrosine hydroxylase increases the affinity for phenylalanine	Rattus norvegicus	14
Reversing the substrate specificities of phenylalanine and tyrosine hydroxylase: aspartate 425 of tyrosine hydroxylase is essential for L-DOPA formation	Rattus norvegicus	4

Records

Records for EC 1.14.16.2 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
tyrosine hydroxylase	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH (not treated with PKA); no dopamine	0.58	0.014	45.7	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase, tetrahydrobiopterin
tyrosine hydroxylase	Homo sapiens	tetrahydrobiopterinno dopamine; BH4 varied 0-200 μM; Table 2	0.47	0.0056	89.3	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase, tetrahydrobiopterin
tyrosine hydroxylase	Homo sapiens	tetrahydrobiopterinphosphorylated TH (phosphorylated by PKA); apparent Km	—	0.507	—	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase, tetrahydrobiopterin
tyrosine hydroxylase	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH; BH4 varied 0-1500 μM; apparent Km	—	0.356	—	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase, tetrahydrobiopterin
tyrosine hydroxylase	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH; BH4 varied 0-1500 μM; apparent kcat	0.017	—	—	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase, tetrahydrobiopterin
tyrosine hydroxylase	Homo sapiens	tetrahydrobiopterinapparent Km; in the text	—	0.134	—	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase, tetrahydrobiopterin
tyrosine hydroxylase	Homo sapiens	tetrahydrobiopterinapparent Km; in the text	—	0.0068	—	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase, tetrahydrobiopterin
tyrosine hydroxylasevariant A297L	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH (not treated with PKA); no dopamine	0.27	0.02	13.7	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant A297L, tetrahydrobiopterin
tyrosine hydroxylasevariant A297L	Homo sapiens	tetrahydrobiopterinno dopamine; BH4 varied 0-200 μM	0.22	0.017	12.5	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant A297L, tetrahydrobiopterin
tyrosine hydroxylasevariant A297L	Homo sapiens	tetrahydrobiopterin	—	0.027	—	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant A297L, tetrahydrobiopterin
tyrosine hydroxylasevariant E332D	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH (not treated with PKA); no dopamine	0.06	0.0083	7.3	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant E332D, tetrahydrobiopterin
tyrosine hydroxylasevariant E332D	Homo sapiens	tetrahydrobiopterinno dopamine; BH4 varied 0-200 μM	0.047	0.0072	6.5	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant E332D, tetrahydrobiopterin
tyrosine hydroxylasevariant E332D	Homo sapiens	tetrahydrobiopterin	—	0.034	—	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant E332D, tetrahydrobiopterin
tyrosine hydroxylasevariant F300A	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH (not treated with PKA)	0.088	0.054	1.7	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant F300A, tetrahydrobiopterin
tyrosine hydroxylasevariant F300A	Homo sapiens	tetrahydrobiopterin	0.112	0.07	1.7	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant F300A, tetrahydrobiopterin
tyrosine hydroxylasevariant F300Y	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH (not treated with PKA)	0.3	0.026	11.2	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant F300Y, tetrahydrobiopterin
tyrosine hydroxylasevariant F300Y	Homo sapiens	tetrahydrobiopterin	0.27	0.012	21.8	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant F300Y, tetrahydrobiopterin
tyrosine hydroxylasevariant L294A	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH (not treated with PKA)	0.18	0.014	13	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant L294A, tetrahydrobiopterin
tyrosine hydroxylasevariant L294A	Homo sapiens	tetrahydrobiopterin	0.142	0.029	5	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant L294A, tetrahydrobiopterin
tyrosine hydroxylasevariant L294Y	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH (not treated with PKA)	0.118	0.015	7.7	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant L294Y, tetrahydrobiopterin
tyrosine hydroxylasevariant L294Y	Homo sapiens	tetrahydrobiopterin	0.068	0.0099	7	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant L294Y, tetrahydrobiopterin
tyrosine hydroxylasevariant Y371F	Homo sapiens	tetrahydrobiopterinnonphosphorylated TH (not treated with PKA); no dopamine	0.2	0.017	11.7	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant Y371F, tetrahydrobiopterin
tyrosine hydroxylasevariant Y371F	Homo sapiens	tetrahydrobiopterinno dopamine; BH4 varied 0-200 μM	0.3	0.029	10.7	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant Y371F, tetrahydrobiopterin
tyrosine hydroxylasevariant Y371F	Homo sapiens	tetrahydrobiopterin	—	0.027	—	pH 7.2, 30 °C	PMID 21235236	Open record: tyrosine hydroxylase variant Y371F, tetrahydrobiopterin
tyrosine hydroxylase	Rattus norvegicus	(6R)-tetrahydrobiopterin	—	0.027	—	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase, (6R)-tetrahydrobiopterin
tyrosine hydroxylase	Rattus norvegicus	6-methyltetrahydropterin	—	0.033	—	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase, 6-methyltetrahydropterin
tyrosine hydroxylase	Rattus norvegicus	6-methyltetrahydropterin	—	0.055	—	pH 6, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase, 6-methyltetrahydropterin
tyrosine hydroxylase	Rattus norvegicus	6-methyltetrahydropterin	—	0.04	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase, 6-methyltetrahydropterin
tyrosine hydroxylase	Rattus norvegicus	L-phenylalaninekcat: the paper does not say per subunit or per oligomer	1.6	0.109	15 calc.	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase, L-phenylalanine
tyrosine hydroxylase	Rattus norvegicus	L-phenylalanine	—	—	17.5	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase, L-phenylalanine
tyrosine hydroxylase	Rattus norvegicus	L-tyrosine	2.52	0.051	49 calc.	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase, L-tyrosine
tyrosine hydroxylase	Rattus norvegicus	L-tyrosine	2.75	0.032	86 calc.	pH 6, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase, L-tyrosine
tyrosine hydroxylase	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.33	0.041	81 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase, L-tyrosine
tyrosine hydroxylase	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.035	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase, L-tyrosine
tyrosine hydroxylase	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase, tetrahydrobiopterin
tyrosine hydroxylasevariant S19A	Rattus norvegicus	6-methyltetrahydropterin	—	0.033	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19A, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S19A	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.33	0.035	95 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19A, L-tyrosine
tyrosine hydroxylasevariant S19A	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.027	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19A, L-tyrosine
tyrosine hydroxylasevariant S19A	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19A, tetrahydrobiopterin
tyrosine hydroxylasevariant S19E	Rattus norvegicus	6-methyltetrahydropterin	—	0.037	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S19E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.83	0.026	150 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19E, L-tyrosine
tyrosine hydroxylasevariant S19E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.033	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19E, L-tyrosine
tyrosine hydroxylasevariant S19E	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19E, tetrahydrobiopterin
tyrosine hydroxylasevariant S19E/S40E	Rattus norvegicus	6-methyltetrahydropterin	—	0.023	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19E/S40E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S19E/S40E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.83	0.059	65 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19E/S40E, L-tyrosine
tyrosine hydroxylasevariant S19E/S40E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.022	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19E/S40E, L-tyrosine
tyrosine hydroxylasevariant S19E/S40E	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S19E/S40E, tetrahydrobiopterin
tyrosine hydroxylasevariant S31A	Rattus norvegicus	6-methyltetrahydropterin	—	0.051	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S31A, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S31A	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.2	0.055	58 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S31A, L-tyrosine
tyrosine hydroxylasevariant S31A	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.041	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S31A, L-tyrosine
tyrosine hydroxylasevariant S31A	Rattus norvegicus	tetrahydrobiopterin	—	0.005	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S31A, tetrahydrobiopterin
tyrosine hydroxylasevariant S31E	Rattus norvegicus	6-methyltetrahydropterin	—	0.047	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S31E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S31E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	4	0.053	75 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S31E, L-tyrosine
tyrosine hydroxylasevariant S31E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.044	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S31E, L-tyrosine
tyrosine hydroxylasevariant S31E	Rattus norvegicus	tetrahydrobiopterin	—	0.008	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S31E, tetrahydrobiopterin
tyrosine hydroxylasevariant S40A	Rattus norvegicus	6-methyltetrahydropterin	—	0.035	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S40A, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S40A	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.17	0.046	69 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S40A, L-tyrosine
tyrosine hydroxylasevariant S40A	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.045	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S40A, L-tyrosine
tyrosine hydroxylasevariant S40A	Rattus norvegicus	tetrahydrobiopterin	—	0.01	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S40A, tetrahydrobiopterin
tyrosine hydroxylasevariant S40E	Rattus norvegicus	6-methyltetrahydropterin	—	0.035	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S40E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S40E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.33	0.047	71 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S40E, L-tyrosine
tyrosine hydroxylasevariant S40E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.034	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S40E, L-tyrosine
tyrosine hydroxylasevariant S40E	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S40E, tetrahydrobiopterin
tyrosine hydroxylasevariant S8A	Rattus norvegicus	6-methyltetrahydropterin	—	0.039	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S8A, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S8A	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	2.5	0.042	60 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S8A, L-tyrosine
tyrosine hydroxylasevariant S8A	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.032	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S8A, L-tyrosine
tyrosine hydroxylasevariant S8A	Rattus norvegicus	tetrahydrobiopterin	—	0.011	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S8A, tetrahydrobiopterin
tyrosine hydroxylasevariant S8E	Rattus norvegicus	6-methyltetrahydropterin	—	0.035	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S8E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S8E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.17	0.047	67 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S8E, L-tyrosine
tyrosine hydroxylasevariant S8E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.025	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S8E, L-tyrosine
tyrosine hydroxylasevariant S8E	Rattus norvegicus	tetrahydrobiopterin	—	0.011	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant S8E, tetrahydrobiopterin
tyrosine hydroxylasevariant TyrHpS31 (phosphorylated at Ser31)	Rattus norvegicus	6-methyltetrahydropterin	—	0.034	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant TyrHpS31 (phosphorylated at Ser31), 6-methyltetrahydropterin
tyrosine hydroxylasevariant TyrHpS31 (phosphorylated at Ser31)	Rattus norvegicus	L-tyrosine	2.95	0.046	64 calc.	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant TyrHpS31 (phosphorylated at Ser31), L-tyrosine
tyrosine hydroxylasevariant TyrHpS31 (phosphorylated at Ser31)	Rattus norvegicus	tetrahydrobiopterin	—	0.016	—	pH 7, 30 °C	PMID 15639226	Open record: tyrosine hydroxylase variant TyrHpS31 (phosphorylated at Ser31), tetrahydrobiopterin
tyrosine hydroxylasevariant Y371F	Rattus norvegicus	(6R)-tetrahydrobiopterin	—	0.053	—	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase variant Y371F, (6R)-tetrahydrobiopterin
tyrosine hydroxylasevariant Y371F	Rattus norvegicus	6-methyltetrahydropterin	—	0.059	—	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase variant Y371F, 6-methyltetrahydropterin
tyrosine hydroxylasevariant Y371F	Rattus norvegicus	6-methyltetrahydropterin	—	0.065	—	pH 6, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase variant Y371F, 6-methyltetrahydropterin
tyrosine hydroxylasevariant Y371F	Rattus norvegicus	L-phenylalaninekcat: the paper does not say per subunit or per oligomer	1.43	0.01	140 calc.	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase variant Y371F, L-phenylalanine
tyrosine hydroxylasevariant Y371F	Rattus norvegicus	L-phenylalanine	—	—	143	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase variant Y371F, L-phenylalanine
tyrosine hydroxylasevariant Y371F	Rattus norvegicus	L-tyrosine	3.58	0.065	55 calc.	pH 7.1, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase variant Y371F, L-tyrosine
tyrosine hydroxylasevariant Y371F	Rattus norvegicus	L-tyrosine	3.52	0.071	50 calc.	pH 6, 30 °C	PMID 9819237	Open record: tyrosine hydroxylase variant Y371F, L-tyrosine
tyrosine hydroxylase (TyrH)	Rattus norvegicus	L-tyrosine	2.5	0.016	156.3	pH 6.7, 32 °C	PMID 10933781	Open record: tyrosine hydroxylase (TyrH), L-tyrosine
tyrosine hydroxylase (TyrH)variant D425V	Rattus norvegicus	L-tyrosine	0.0207	0.045	0.47	pH 6.7, 32 °C	PMID 10933781	Open record: tyrosine hydroxylase (TyrH) variant D425V, L-tyrosine
tyrosine hydroxylase (TyrH)variant H323Y	Rattus norvegicus	L-tyrosine	2.65	0.092	28.8	pH 6.7, 32 °C	PMID 10933781	Open record: tyrosine hydroxylase (TyrH) variant H323Y, L-tyrosine
tyrosine hydroxylase (TyrH)variant Q310H	Rattus norvegicus	L-tyrosine	2.13	0.054	39.5	pH 6.7, 32 °C	PMID 10933781	Open record: tyrosine hydroxylase (TyrH) variant Q310H, L-tyrosine