Values CatLog read from this paper

Mutation of regulatory serines of rat tyrosine hydroxylase to glutamate: effects on enzyme stability and activity

PMID 15639226doi:10.1016/j.abb.2004.11.007Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 43 records (54 values) from this paper: tyrosine hydroxylase from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 11 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.14.16.2

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
tyrosine hydroxylase	Rattus norvegicus	6-methyltetrahydropterin	—	0.04	—	pH 7, 30 °C	Open record: tyrosine hydroxylase, 6-methyltetrahydropterin
tyrosine hydroxylase	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.33	0.041	81 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase, L-tyrosine
tyrosine hydroxylase	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.035	—	pH 7, 30 °C	Open record: tyrosine hydroxylase, L-tyrosine
tyrosine hydroxylase	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	Open record: tyrosine hydroxylase, tetrahydrobiopterin
tyrosine hydroxylasevariant S19A	Rattus norvegicus	6-methyltetrahydropterin	—	0.033	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19A, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S19A	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.33	0.035	95 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19A, L-tyrosine
tyrosine hydroxylasevariant S19A	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.027	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19A, L-tyrosine
tyrosine hydroxylasevariant S19A	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19A, tetrahydrobiopterin
tyrosine hydroxylasevariant S19E	Rattus norvegicus	6-methyltetrahydropterin	—	0.037	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S19E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.83	0.026	150 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19E, L-tyrosine
tyrosine hydroxylasevariant S19E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.033	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19E, L-tyrosine
tyrosine hydroxylasevariant S19E	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19E, tetrahydrobiopterin
tyrosine hydroxylasevariant S19E/S40E	Rattus norvegicus	6-methyltetrahydropterin	—	0.023	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19E/S40E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S19E/S40E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.83	0.059	65 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19E/S40E, L-tyrosine
tyrosine hydroxylasevariant S19E/S40E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.022	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19E/S40E, L-tyrosine
tyrosine hydroxylasevariant S19E/S40E	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S19E/S40E, tetrahydrobiopterin
tyrosine hydroxylasevariant S31A	Rattus norvegicus	6-methyltetrahydropterin	—	0.051	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S31A, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S31A	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.2	0.055	58 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S31A, L-tyrosine
tyrosine hydroxylasevariant S31A	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.041	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S31A, L-tyrosine
tyrosine hydroxylasevariant S31A	Rattus norvegicus	tetrahydrobiopterin	—	0.005	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S31A, tetrahydrobiopterin
tyrosine hydroxylasevariant S31E	Rattus norvegicus	6-methyltetrahydropterin	—	0.047	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S31E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S31E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	4	0.053	75 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S31E, L-tyrosine
tyrosine hydroxylasevariant S31E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.044	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S31E, L-tyrosine
tyrosine hydroxylasevariant S31E	Rattus norvegicus	tetrahydrobiopterin	—	0.008	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S31E, tetrahydrobiopterin
tyrosine hydroxylasevariant S40A	Rattus norvegicus	6-methyltetrahydropterin	—	0.035	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S40A, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S40A	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.17	0.046	69 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S40A, L-tyrosine
tyrosine hydroxylasevariant S40A	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.045	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S40A, L-tyrosine
tyrosine hydroxylasevariant S40A	Rattus norvegicus	tetrahydrobiopterin	—	0.01	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S40A, tetrahydrobiopterin
tyrosine hydroxylasevariant S40E	Rattus norvegicus	6-methyltetrahydropterin	—	0.035	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S40E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S40E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.33	0.047	71 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S40E, L-tyrosine
tyrosine hydroxylasevariant S40E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.034	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S40E, L-tyrosine
tyrosine hydroxylasevariant S40E	Rattus norvegicus	tetrahydrobiopterin	—	0.009	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S40E, tetrahydrobiopterin
tyrosine hydroxylasevariant S8A	Rattus norvegicus	6-methyltetrahydropterin	—	0.039	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S8A, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S8A	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	2.5	0.042	60 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S8A, L-tyrosine
tyrosine hydroxylasevariant S8A	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.032	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S8A, L-tyrosine
tyrosine hydroxylasevariant S8A	Rattus norvegicus	tetrahydrobiopterin	—	0.011	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S8A, tetrahydrobiopterin
tyrosine hydroxylasevariant S8E	Rattus norvegicus	6-methyltetrahydropterin	—	0.035	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S8E, 6-methyltetrahydropterin
tyrosine hydroxylasevariant S8E	Rattus norvegicus	L-tyrosineinitial rates fit to Michaelis-Menten equation	3.17	0.047	67 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S8E, L-tyrosine
tyrosine hydroxylasevariant S8E	Rattus norvegicus	L-tyrosineiron-reconstituted recombinant enzyme (E. coli)	—	0.025	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S8E, L-tyrosine
tyrosine hydroxylasevariant S8E	Rattus norvegicus	tetrahydrobiopterin	—	0.011	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant S8E, tetrahydrobiopterin
tyrosine hydroxylasevariant TyrHpS31 (phosphorylated at Ser31)	Rattus norvegicus	6-methyltetrahydropterin	—	0.034	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant TyrHpS31 (phosphorylated at Ser31), 6-methyltetrahydropterin
tyrosine hydroxylasevariant TyrHpS31 (phosphorylated at Ser31)	Rattus norvegicus	L-tyrosine	2.95	0.046	64 calc.	pH 7, 30 °C	Open record: tyrosine hydroxylase variant TyrHpS31 (phosphorylated at Ser31), L-tyrosine
tyrosine hydroxylasevariant TyrHpS31 (phosphorylated at Ser31)	Rattus norvegicus	tetrahydrobiopterin	—	0.016	—	pH 7, 30 °C	Open record: tyrosine hydroxylase variant TyrHpS31 (phosphorylated at Ser31), tetrahydrobiopterin