Values CatLog read from this paper
Mutation to phenylalanine of tyrosine 371 in tyrosine hydroxylase increases the affinity for phenylalanine
PMID 9819237doi:10.1021/bi981648fOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (20 values) from this paper: tyrosine hydroxylase from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tyrosine hydroxylase | Rattus norvegicus | (6R)-tetrahydrobiopterin | — | 0.027 | — | pH 7.1, 30 °C | Open record: tyrosine hydroxylase, (6R)-tetrahydrobiopterin |
| tyrosine hydroxylase | Rattus norvegicus | 6-methyltetrahydropterin | — | 0.033 | — | pH 7.1, 30 °C | Open record: tyrosine hydroxylase, 6-methyltetrahydropterin |
| tyrosine hydroxylase | Rattus norvegicus | 6-methyltetrahydropterin | — | 0.055 | — | pH 6, 30 °C | Open record: tyrosine hydroxylase, 6-methyltetrahydropterin |
| tyrosine hydroxylase | Rattus norvegicus | L-phenylalaninekcat: the paper does not say per subunit or per oligomer | 1.6 | 0.109 | 15 calc. | pH 7.1, 30 °C | Open record: tyrosine hydroxylase, L-phenylalanine |
| tyrosine hydroxylase | Rattus norvegicus | L-phenylalanine | — | — | 17.5 | pH 7.1, 30 °C | Open record: tyrosine hydroxylase, L-phenylalanine |
| tyrosine hydroxylase | Rattus norvegicus | L-tyrosine | 2.52 | 0.051 | 49 calc. | pH 7.1, 30 °C | Open record: tyrosine hydroxylase, L-tyrosine |
| tyrosine hydroxylase | Rattus norvegicus | L-tyrosine | 2.75 | 0.032 | 86 calc. | pH 6, 30 °C | Open record: tyrosine hydroxylase, L-tyrosine |
| tyrosine hydroxylasevariant Y371F | Rattus norvegicus | (6R)-tetrahydrobiopterin | — | 0.053 | — | pH 7.1, 30 °C | Open record: tyrosine hydroxylase variant Y371F, (6R)-tetrahydrobiopterin |
| tyrosine hydroxylasevariant Y371F | Rattus norvegicus | 6-methyltetrahydropterin | — | 0.059 | — | pH 7.1, 30 °C | Open record: tyrosine hydroxylase variant Y371F, 6-methyltetrahydropterin |
| tyrosine hydroxylasevariant Y371F | Rattus norvegicus | 6-methyltetrahydropterin | — | 0.065 | — | pH 6, 30 °C | Open record: tyrosine hydroxylase variant Y371F, 6-methyltetrahydropterin |
| tyrosine hydroxylasevariant Y371F | Rattus norvegicus | L-phenylalaninekcat: the paper does not say per subunit or per oligomer | 1.43 | 0.01 | 140 calc. | pH 7.1, 30 °C | Open record: tyrosine hydroxylase variant Y371F, L-phenylalanine |
| tyrosine hydroxylasevariant Y371F | Rattus norvegicus | L-phenylalanine | — | — | 143 | pH 7.1, 30 °C | Open record: tyrosine hydroxylase variant Y371F, L-phenylalanine |
| tyrosine hydroxylasevariant Y371F | Rattus norvegicus | L-tyrosine | 3.58 | 0.065 | 55 calc. | pH 7.1, 30 °C | Open record: tyrosine hydroxylase variant Y371F, L-tyrosine |
| tyrosine hydroxylasevariant Y371F | Rattus norvegicus | L-tyrosine | 3.52 | 0.071 | 50 calc. | pH 6, 30 °C | Open record: tyrosine hydroxylase variant Y371F, L-tyrosine |