EC 4.2.99.21: isochorismate lyase
LyasesOpen in Browse
CatLog read 30 records for EC 4.2.99.21 from 2 papers, for 2 organisms. Names in these papers: PchB, Irp9 (salicylate synthase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 17 records for EC 4.2.99.21 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.2.99.21 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection | Pseudomonas aeruginosa | 24 |
| Redesign of MST enzymes to target lyase activity instead promotes mutase and dehydratase activities | Yersinia enterocolitica | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| Irp9 (salicylate synthase) | Yersinia enterocolitica | chorismate | 0.084 | 0.00198 | 42 | pH 7.5, 25 °C | PMID 24055536 | Open record: Irp9 (salicylate synthase), chorismate |
| Irp9 (salicylate synthase) | Yersinia enterocolitica | isochorismate | 0.092 | 5.8 × 10⁻⁴ | 159 | pH 7.5, 25 °C | PMID 24055536 | Open record: Irp9 (salicylate synthase), isochorismate |
| Irp9 (salicylate synthase)variant E281D | Yersinia enterocolitica | chorismate | 0.008 | 0.006 | 1.3 | pH 7.5, 25 °C | PMID 24055536 | Open record: Irp9 (salicylate synthase) variant E281D, chorismate |
| Irp9 (salicylate synthase)variant E281D | Yersinia enterocolitica | isochorismate | 0.0504 | 0.0017 | 31 | pH 7.5, 25 °C | PMID 24055536 | Open record: Irp9 (salicylate synthase) variant E281D, isochorismate |
| Irp9 (salicylate synthase)variant E281D/T348A | Yersinia enterocolitica | isochorismate | 0.0343 | 0.047 | 0.74 | pH 7.5, 25 °C | PMID 24055536 | Open record: Irp9 (salicylate synthase) variant E281D/T348A, isochorismate |
| Irp9 (salicylate synthase)variant T348A | Yersinia enterocolitica | isochorismate | 0.0055 | 5.9 × 10⁻⁴ | 9.3 | pH 7.5, 25 °C | PMID 24055536 | Open record: Irp9 (salicylate synthase) variant T348A, isochorismate |
| PchB | Pseudomonas aeruginosa | chorismate | 0.085 | — | 2.2 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB, chorismate |
| PchB | Pseudomonas aeruginosa | chorismate | — | 0.038 | — | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB, chorismate |
| PchB | Pseudomonas aeruginosa | isochorismate | 1.06 | — | 1.13 × 10³ | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB, isochorismate |
| PchB | Pseudomonas aeruginosa | isochorismate | — | 9.5 × 10⁻⁴ | — | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB, isochorismate |
| PchBvariant E90F | Pseudomonas aeruginosa | chorismate | 0.051 | — | 0.87 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90F, chorismate |
| PchBvariant E90F | Pseudomonas aeruginosa | isochorismate | 0.39 | — | 300 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90F, isochorismate |
| PchBvariant E90F/Q91S | Pseudomonas aeruginosa | chorismate | 0.0073 | — | 0.11 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90F/Q91S, chorismate |
| PchBvariant E90F/Q91S | Pseudomonas aeruginosa | isochorismate | 0.022 | — | 8.8 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90F/Q91S, isochorismate |
| PchBvariant E90H/Q91A | Pseudomonas aeruginosa | chorismate | 0.0081 | — | 0.26 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90H/Q91A, chorismate |
| PchBvariant E90H/Q91A | Pseudomonas aeruginosa | isochorismate | 0.075 | — | 21 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90H/Q91A, isochorismate |
| PchBvariant E90K | Pseudomonas aeruginosa | chorismate | 0.089 | — | 1.7 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90K, chorismate |
| PchBvariant E90K | Pseudomonas aeruginosa | isochorismate | 1.01 | — | 740 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90K, isochorismate |
| PchBvariant E90K/Q91S | Pseudomonas aeruginosa | chorismate | 0.023 | — | 0.36 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90K/Q91S, chorismate |
| PchBvariant E90K/Q91S | Pseudomonas aeruginosa | isochorismate | 0.11 | — | 53 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90K/Q91S, isochorismate |
| PchBvariant E90S | Pseudomonas aeruginosa | chorismate | 0.068 | — | 1.3 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90S, chorismate |
| PchBvariant E90S | Pseudomonas aeruginosa | isochorismate | 0.77 | — | 570 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90S, isochorismate |
| PchBvariant E90Y/Q91E | Pseudomonas aeruginosa | chorismate | 1.5 | — | 0.46 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90Y/Q91E, chorismate |
| PchBvariant E90Y/Q91E | Pseudomonas aeruginosa | isochorismate | 3 | — | 6.3 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant E90Y/Q91E, isochorismate |
| PchBvariant Q91E | Pseudomonas aeruginosa | chorismate | 0.9 | — | 0.6 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant Q91E, chorismate |
| PchBvariant Q91E | Pseudomonas aeruginosa | isochorismate | 3 | — | 10.4 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant Q91E, isochorismate |
| PchBvariant Q91N | Pseudomonas aeruginosa | chorismate | 0.0062 | — | 0.083 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant Q91N, chorismate |
| PchBvariant Q91N | Pseudomonas aeruginosa | isochorismate | 0.27 | — | 57 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant Q91N, isochorismate |
| PchBvariant R54K | Pseudomonas aeruginosa | chorismate | 0.003 | — | 0.0023 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant R54K, chorismate |
| PchBvariant R54K | Pseudomonas aeruginosa | isochorismate | 0.1 | — | 0.9 | pH 7.5, 30 °C | PMID 16036918 | Open record: PchB variant R54K, isochorismate |