Values CatLog read from this paper
Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection
PMID 16036918doi:10.1074/jbc.m506883200Open in Browse
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CatLog read 25 records (48 values) from this paper: PchB, EcCM, the chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase from Pseudomonas aeruginosa, Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| EcCM, the chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase | Escherichia coli | chorismate | 55 | — | 230 | pH 7.5, 30 °C | Open record: EcCM, the chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase, chorismate |
| PchB | Pseudomonas aeruginosa | chorismate | 0.085 | — | 2.2 | pH 7.5, 30 °C | Open record: PchB, chorismate |
| PchB | Pseudomonas aeruginosa | chorismate | — | 0.038 | — | pH 7.5, 30 °C | Open record: PchB, chorismate |
| PchB | Pseudomonas aeruginosa | isochorismate | 1.06 | — | 1.13 × 10³ | pH 7.5, 30 °C | Open record: PchB, isochorismate |
| PchB | Pseudomonas aeruginosa | isochorismate | — | 9.5 × 10⁻⁴ | — | pH 7.5, 30 °C | Open record: PchB, isochorismate |
| PchBvariant E90F | Pseudomonas aeruginosa | chorismate | 0.051 | — | 0.87 | pH 7.5, 30 °C | Open record: PchB variant E90F, chorismate |
| PchBvariant E90F | Pseudomonas aeruginosa | isochorismate | 0.39 | — | 300 | pH 7.5, 30 °C | Open record: PchB variant E90F, isochorismate |
| PchBvariant E90F/Q91S | Pseudomonas aeruginosa | chorismate | 0.0073 | — | 0.11 | pH 7.5, 30 °C | Open record: PchB variant E90F/Q91S, chorismate |
| PchBvariant E90F/Q91S | Pseudomonas aeruginosa | isochorismate | 0.022 | — | 8.8 | pH 7.5, 30 °C | Open record: PchB variant E90F/Q91S, isochorismate |
| PchBvariant E90H/Q91A | Pseudomonas aeruginosa | chorismate | 0.0081 | — | 0.26 | pH 7.5, 30 °C | Open record: PchB variant E90H/Q91A, chorismate |
| PchBvariant E90H/Q91A | Pseudomonas aeruginosa | isochorismate | 0.075 | — | 21 | pH 7.5, 30 °C | Open record: PchB variant E90H/Q91A, isochorismate |
| PchBvariant E90K | Pseudomonas aeruginosa | chorismate | 0.089 | — | 1.7 | pH 7.5, 30 °C | Open record: PchB variant E90K, chorismate |
| PchBvariant E90K | Pseudomonas aeruginosa | isochorismate | 1.01 | — | 740 | pH 7.5, 30 °C | Open record: PchB variant E90K, isochorismate |
| PchBvariant E90K/Q91S | Pseudomonas aeruginosa | chorismate | 0.023 | — | 0.36 | pH 7.5, 30 °C | Open record: PchB variant E90K/Q91S, chorismate |
| PchBvariant E90K/Q91S | Pseudomonas aeruginosa | isochorismate | 0.11 | — | 53 | pH 7.5, 30 °C | Open record: PchB variant E90K/Q91S, isochorismate |
| PchBvariant E90S | Pseudomonas aeruginosa | chorismate | 0.068 | — | 1.3 | pH 7.5, 30 °C | Open record: PchB variant E90S, chorismate |
| PchBvariant E90S | Pseudomonas aeruginosa | isochorismate | 0.77 | — | 570 | pH 7.5, 30 °C | Open record: PchB variant E90S, isochorismate |
| PchBvariant E90Y/Q91E | Pseudomonas aeruginosa | chorismate | 1.5 | — | 0.46 | pH 7.5, 30 °C | Open record: PchB variant E90Y/Q91E, chorismate |
| PchBvariant E90Y/Q91E | Pseudomonas aeruginosa | isochorismate | 3 | — | 6.3 | pH 7.5, 30 °C | Open record: PchB variant E90Y/Q91E, isochorismate |
| PchBvariant Q91E | Pseudomonas aeruginosa | chorismate | 0.9 | — | 0.6 | pH 7.5, 30 °C | Open record: PchB variant Q91E, chorismate |
| PchBvariant Q91E | Pseudomonas aeruginosa | isochorismate | 3 | — | 10.4 | pH 7.5, 30 °C | Open record: PchB variant Q91E, isochorismate |
| PchBvariant Q91N | Pseudomonas aeruginosa | chorismate | 0.0062 | — | 0.083 | pH 7.5, 30 °C | Open record: PchB variant Q91N, chorismate |
| PchBvariant Q91N | Pseudomonas aeruginosa | isochorismate | 0.27 | — | 57 | pH 7.5, 30 °C | Open record: PchB variant Q91N, isochorismate |
| PchBvariant R54K | Pseudomonas aeruginosa | chorismate | 0.003 | — | 0.0023 | pH 7.5, 30 °C | Open record: PchB variant R54K, chorismate |
| PchBvariant R54K | Pseudomonas aeruginosa | isochorismate | 0.1 | — | 0.9 | pH 7.5, 30 °C | Open record: PchB variant R54K, isochorismate |