EC 5.4.99.5: chorismate mutase

IsomerasesOpen in Browse

CatLog read 90 records for EC 5.4.99.5 from 7 papers, for 3 organisms. Names in these papers: chorismate mutase-prephenate dehydrogenase, PchB, P-protein (bifunctional chorismate mutase/prephenate dehydratase), chorismate mutase (BsCM, monofunctional) and 5 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 167 records for EC 5.4.99.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 5.4.99.5 in Browse.

Papers

Papers with records for EC 5.4.99.5
PaperOrganismsRecords
Use of site-directed mutagenesis to identify residues specific for each reaction catalyzed by chorismate mutase-prephenate dehydrogenase from Escherichia coliEscherichia coli31
Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selectionEscherichia coli, Pseudomonas aeruginosa25
Bacillus subtilis chorismate mutase is partially diffusion-controlledBacillus subtilis, Escherichia coli11
Probing the catalytic mechanism of prephenate dehydratase by site-directed mutagenesis of the Escherichia coli P-protein dehydratase domainEscherichia coli10
Probing the role of the C-terminus of Bacillus subtilis chorismate mutase by a novel random protein-termination strategyBacillus subtilis7
A strategically positioned cation is crucial for efficient catalysis by chorismate mutaseBacillus subtilis3
Chorismate mutase-prephenate dehydratase from Escherichia coli. Study of catalytic and regulatory domains using genetically engineered proteinsEscherichia coli3

Records

Records for EC 5.4.99.5 read from the paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaperOpen record
chorismate mutase (BsCM, AroH class)variant C88K/R90SBacillus subtilischorismate0.321.90.17pH 7.5, 30 °CPMID 10960481Open record: chorismate mutase (BsCM, AroH class) variant C88K/R90S, chorismate
chorismate mutase (BsCM, AroH class)variant C88S/R90KBacillus subtilischorismate0.294.30.067pH 7.5, 30 °CPMID 10960481Open record: chorismate mutase (BsCM, AroH class) variant C88S/R90K, chorismate
chorismate mutase (BsCM, AroH class)variant R90GBacillus subtilischorismate2.7 × 10⁻⁴0.150.0018pH 7.5, 30 °CPMID 10960481Open record: chorismate mutase (BsCM, AroH class) variant R90G, chorismate
chorismate mutase (BsCM, AroH)Bacillus subtilischorismate470.081580pH 7.5, 30 °CPMID 11087356Open record: chorismate mutase (BsCM, AroH), chorismate
chorismate mutase (BsCM, AroH)variant D118*Bacillus subtilischorismatehighest chorismate concentration used 5.8 mM; clone 4-5223.95.7pH 7.5, 30 °CPMID 11087356Open record: chorismate mutase (BsCM, AroH) variant D118*, chorismate
chorismate mutase (BsCM, AroH)variant D118*Bacillus subtilischorismatehighest chorismate concentration used 3.4 mM; clone 6-9244.15.7pH 7.5, 30 °CPMID 11087356Open record: chorismate mutase (BsCM, AroH) variant D118*, chorismate
chorismate mutase (BsCM, AroH)variant D118N/L119*Bacillus subtilischorismate2345.6pH 7.5, 30 °CPMID 11087356Open record: chorismate mutase (BsCM, AroH) variant D118N/L119*, chorismate
chorismate mutase (BsCM, AroH)variant K111N/A112S/V113N/D118*Bacillus subtilischorismate30152pH 7.5, 30 °CPMID 11087356Open record: chorismate mutase (BsCM, AroH) variant K111N/A112S/V113N/D118*, chorismate
chorismate mutase (BsCM, AroH)variant P117*Bacillus subtilischorismate269.32.8pH 7.5, 30 °CPMID 11087356Open record: chorismate mutase (BsCM, AroH) variant P117*, chorismate
chorismate mutase (BsCM, AroH)variant R116L/P117T/D118*Bacillus subtilischorismate26161.6pH 7.5, 30 °CPMID 11087356Open record: chorismate mutase (BsCM, AroH) variant R116L/P117T/D118*, chorismate
chorismate mutase (BsCM, monofunctional)Bacillus subtilischorismateno viscosogen (relative viscosity 1.00)500.085590pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase (BsCM, monofunctional), chorismate
chorismate mutase (BsCM, monofunctional)Bacillus subtilischorismate3.8% (w/w) PEG 8000; relative viscosity 2.09460.084550pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase (BsCM, monofunctional), chorismate
chorismate mutase (BsCM, monofunctional)Bacillus subtilischorismate5.7% (w/w) PEG 8000; relative viscosity 2.79450.076590pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase (BsCM, monofunctional), chorismate
chorismate mutase (BsCM, monofunctional)Bacillus subtilischorismate7.0% (w/w) PEG 8000; relative viscosity 3.43430.076570pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase (BsCM, monofunctional), chorismate
chorismate mutase (BsCM, monofunctional)Bacillus subtilischorismate15.3% (w/w) PEG 2000; relative viscosity 3.30470.091520pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase (BsCM, monofunctional), chorismate
chorismate mutase (BsCM, monofunctional)Bacillus subtilischorismate13.0% (w/w) ficoll; relative viscosity 7.81440.069640pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase (BsCM, monofunctional), chorismate
chorismate mutase (BsCM, monofunctional)variant C75ABacillus subtilischorismate591.0656pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase (BsCM, monofunctional) variant C75A, chorismate
chorismate mutase (BsCM, monofunctional)variant C75SBacillus subtilischorismate760.56140pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase (BsCM, monofunctional) variant C75S, chorismate
chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase (EcCM, AroQp)Escherichia colichorismateno viscosogen690.3230pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase (EcCM, AroQp), chorismate
chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase (EcCM, AroQp)Escherichia colichorismate44.4% (w/w) glycerol (relative viscosity about 3.9)740.32230pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase (EcCM, AroQp), chorismate
chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase (EcCM, AroQp)Escherichia colichorismate33.3% (w/w) sucrose (relative viscosity about 3.9)710.16460pH 7.5, 30 °CPMID 10103029Open record: chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase (EcCM, AroQp), chorismate
chorismate mutase-prephenate dehydratase (P-protein), chorismate mutase activityEscherichia colichorismate39.030.22617337 °CPMID 9497350Open record: chorismate mutase-prephenate dehydratase (P-protein), chorismate mutase activity, chorismate
chorismate mutase-prephenate dehydratase (P-protein), chorismate mutase activityP*285 (C-terminal truncation, residues 1-285)Escherichia colichorismate44.251.1713837 °CPMID 9497350Open record: chorismate mutase-prephenate dehydratase (P-protein), chorismate mutase activity P*285 (C-terminal truncation, residues 1-285), chorismate
chorismate mutase-prephenate dehydratase (P-protein), chorismate mutase activityP*300 (C-terminal truncation, residues 1-300)Escherichia colichorismate40.70.7055837 °CPMID 9497350Open record: chorismate mutase-prephenate dehydratase (P-protein), chorismate mutase activity P*300 (C-terminal truncation, residues 1-300), chorismate
chorismate mutase-prephenate dehydrogenaseEscherichia colichorismate270.045600pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase, chorismate
chorismate mutase-prephenate dehydrogenaseEscherichia colinicotinamide adenine dinucleotide270.103260pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenaseEscherichia coliprephenate270.044620pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase, prephenate
chorismate mutase-prephenate dehydrogenaseEscherichia coliprephenate80—1.46 × 10³30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase, prephenate
chorismate mutase-prephenate dehydrogenasevariant H131AEscherichia colichorismate7.20.053140pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H131A, chorismate
chorismate mutase-prephenate dehydrogenasevariant H131AEscherichia colinicotinamide adenine dinucleotide2.60.289.3pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H131A, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H131AEscherichia coliprephenate3.10.05660pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H131A, prephenate
chorismate mutase-prephenate dehydrogenasevariant H153NEscherichia colichorismate100.051200pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H153N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H153NEscherichia colinicotinamide adenine dinucleotide190.171110pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H153N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H153NEscherichia coliprephenate190.046420pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H153N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H197NEscherichia colichorismate160.068230pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H197N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H197NEscherichia colinicotinamide adenine dinucleotide0.00350.1280.0027pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H197N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H197NEscherichia coliprephenate0.0030.0550.055pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H197N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H197NEscherichia coliprephenate0.003——30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H197N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H238NEscherichia colichorismate2.30.12618pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H238N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H238NEscherichia colinicotinamide adenine dinucleotide5.60.18630pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H238N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H238NEscherichia coliprephenate5.60.034160pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H238N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H245NEscherichia colichorismate4.80.22521pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H245N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H245NEscherichia colinicotinamide adenine dinucleotide150.18580pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H245N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H245NEscherichia coliprephenate120.1120pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H245N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H257AEscherichia colichorismate80.09881pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H257A, chorismate
chorismate mutase-prephenate dehydrogenasevariant H257AEscherichia colinicotinamide adenine dinucleotide150.142100pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H257A, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H257AEscherichia coliprephenate200.099200pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H257A, prephenate
chorismate mutase-prephenate dehydrogenasevariant H265AEscherichia colichorismate150.099150pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H265A, chorismate
chorismate mutase-prephenate dehydrogenasevariant H265AEscherichia colinicotinamide adenine dinucleotide7.20.19140pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H265A, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H265AEscherichia coliprephenate80.077100pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H265A, prephenate
chorismate mutase-prephenate dehydrogenasevariant H347NEscherichia colichorismate60.041150pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H347N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H347NEscherichia colinicotinamide adenine dinucleotide4.50.07360pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H347N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H347NEscherichia coliprephenate9.80.028350pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant H347N, prephenate
chorismate mutase-prephenate dehydrogenasevariant K37QEscherichia colinicotinamide adenine dinucleotide220.141160pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant K37Q, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant K37QEscherichia coliprephenate230.054430pH 7.2, 30 °CPMID 9843375Open record: chorismate mutase-prephenate dehydrogenase variant K37Q, prephenate
EcCM, the chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydrataseEscherichia colichorismate55—230pH 7.5, 30 °CPMID 16036918Open record: EcCM, the chorismate mutase domain of the bifunctional chorismate mutase-prephenate dehydratase, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)Escherichia colichorismate34.180.12726837 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase), chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant C216AEscherichia colichorismate33.550.15921237 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant C216A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant C216SEscherichia colichorismate32.450.11328737 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant C216S, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant E159AEscherichia colichorismate34.220.12427537 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant E159A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant N160AEscherichia colichorismate33.550.15122237 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant N160A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant Q215AEscherichia colichorismate35.130.15422837 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant Q215A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant T278AEscherichia colichorismate35.520.12328837 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant T278A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant T278SEscherichia colichorismate34.120.12327737 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant T278S, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant T278VEscherichia colichorismate32.270.11528037 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant T278V, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant W226AEscherichia colichorismate32.60.13923537 °CPMID 10769128Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant W226A, chorismate
PchBPseudomonas aeruginosachorismate0.085—2.2pH 7.5, 30 °CPMID 16036918Open record: PchB, chorismate
PchBPseudomonas aeruginosachorismate—0.038—pH 7.5, 30 °CPMID 16036918Open record: PchB, chorismate
PchBPseudomonas aeruginosaisochorismate1.06—1.13 × 10³pH 7.5, 30 °CPMID 16036918Open record: PchB, isochorismate
PchBPseudomonas aeruginosaisochorismate—9.5 × 10⁻⁴—pH 7.5, 30 °CPMID 16036918Open record: PchB, isochorismate
PchBvariant E90FPseudomonas aeruginosachorismate0.051—0.87pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90F, chorismate
PchBvariant E90FPseudomonas aeruginosaisochorismate0.39—300pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90F, isochorismate
PchBvariant E90F/Q91SPseudomonas aeruginosachorismate0.0073—0.11pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90F/Q91S, chorismate
PchBvariant E90F/Q91SPseudomonas aeruginosaisochorismate0.022—8.8pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90F/Q91S, isochorismate
PchBvariant E90H/Q91APseudomonas aeruginosachorismate0.0081—0.26pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90H/Q91A, chorismate
PchBvariant E90H/Q91APseudomonas aeruginosaisochorismate0.075—21pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90H/Q91A, isochorismate
PchBvariant E90KPseudomonas aeruginosachorismate0.089—1.7pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90K, chorismate
PchBvariant E90KPseudomonas aeruginosaisochorismate1.01—740pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90K, isochorismate
PchBvariant E90K/Q91SPseudomonas aeruginosachorismate0.023—0.36pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90K/Q91S, chorismate
PchBvariant E90K/Q91SPseudomonas aeruginosaisochorismate0.11—53pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90K/Q91S, isochorismate
PchBvariant E90SPseudomonas aeruginosachorismate0.068—1.3pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90S, chorismate
PchBvariant E90SPseudomonas aeruginosaisochorismate0.77—570pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90S, isochorismate
PchBvariant E90Y/Q91EPseudomonas aeruginosachorismate1.5—0.46pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90Y/Q91E, chorismate
PchBvariant E90Y/Q91EPseudomonas aeruginosaisochorismate3—6.3pH 7.5, 30 °CPMID 16036918Open record: PchB variant E90Y/Q91E, isochorismate
PchBvariant Q91EPseudomonas aeruginosachorismate0.9—0.6pH 7.5, 30 °CPMID 16036918Open record: PchB variant Q91E, chorismate
PchBvariant Q91EPseudomonas aeruginosaisochorismate3—10.4pH 7.5, 30 °CPMID 16036918Open record: PchB variant Q91E, isochorismate
PchBvariant Q91NPseudomonas aeruginosachorismate0.0062—0.083pH 7.5, 30 °CPMID 16036918Open record: PchB variant Q91N, chorismate
PchBvariant Q91NPseudomonas aeruginosaisochorismate0.27—57pH 7.5, 30 °CPMID 16036918Open record: PchB variant Q91N, isochorismate
PchBvariant R54KPseudomonas aeruginosachorismate0.003—0.0023pH 7.5, 30 °CPMID 16036918Open record: PchB variant R54K, chorismate
PchBvariant R54KPseudomonas aeruginosaisochorismate0.1—0.9pH 7.5, 30 °CPMID 16036918Open record: PchB variant R54K, isochorismate