Values CatLog read from this paper

Use of site-directed mutagenesis to identify residues specific for each reaction catalyzed by chorismate mutase-prephenate dehydrogenase from Escherichia coli

PMID 9843375doi:10.1021/bi981412bOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 31 records (90 values) from this paper: chorismate mutase-prephenate dehydrogenase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.3.1.12, EC 5.4.99.5

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
chorismate mutase-prephenate dehydrogenase	Escherichia coli	chorismate	27	0.045	600	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase, chorismate
chorismate mutase-prephenate dehydrogenase	Escherichia coli	nicotinamide adenine dinucleotide	27	0.103	260	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenase	Escherichia coli	prephenate	27	0.044	620	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase, prephenate
chorismate mutase-prephenate dehydrogenase	Escherichia coli	prephenate	80	—	1.46 × 10³	30 °C	Open record: chorismate mutase-prephenate dehydrogenase, prephenate
chorismate mutase-prephenate dehydrogenasevariant H131A	Escherichia coli	chorismate	7.2	0.053	140	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H131A, chorismate
chorismate mutase-prephenate dehydrogenasevariant H131A	Escherichia coli	nicotinamide adenine dinucleotide	2.6	0.28	9.3	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H131A, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H131A	Escherichia coli	prephenate	3.1	0.056	60	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H131A, prephenate
chorismate mutase-prephenate dehydrogenasevariant H153N	Escherichia coli	chorismate	10	0.051	200	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H153N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H153N	Escherichia coli	nicotinamide adenine dinucleotide	19	0.171	110	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H153N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H153N	Escherichia coli	prephenate	19	0.046	420	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H153N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H197N	Escherichia coli	chorismate	16	0.068	230	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H197N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H197N	Escherichia coli	nicotinamide adenine dinucleotide	0.0035	0.128	0.0027	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H197N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H197N	Escherichia coli	prephenate	0.003	0.055	0.055	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H197N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H197N	Escherichia coli	prephenate	0.003	—	—	30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H197N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H238N	Escherichia coli	chorismate	2.3	0.126	18	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H238N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H238N	Escherichia coli	nicotinamide adenine dinucleotide	5.6	0.186	30	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H238N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H238N	Escherichia coli	prephenate	5.6	0.034	160	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H238N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H245N	Escherichia coli	chorismate	4.8	0.225	21	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H245N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H245N	Escherichia coli	nicotinamide adenine dinucleotide	15	0.185	80	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H245N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H245N	Escherichia coli	prephenate	12	0.1	120	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H245N, prephenate
chorismate mutase-prephenate dehydrogenasevariant H257A	Escherichia coli	chorismate	8	0.098	81	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H257A, chorismate
chorismate mutase-prephenate dehydrogenasevariant H257A	Escherichia coli	nicotinamide adenine dinucleotide	15	0.142	100	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H257A, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H257A	Escherichia coli	prephenate	20	0.099	200	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H257A, prephenate
chorismate mutase-prephenate dehydrogenasevariant H265A	Escherichia coli	chorismate	15	0.099	150	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H265A, chorismate
chorismate mutase-prephenate dehydrogenasevariant H265A	Escherichia coli	nicotinamide adenine dinucleotide	7.2	0.191	40	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H265A, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H265A	Escherichia coli	prephenate	8	0.077	100	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H265A, prephenate
chorismate mutase-prephenate dehydrogenasevariant H347N	Escherichia coli	chorismate	6	0.041	150	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H347N, chorismate
chorismate mutase-prephenate dehydrogenasevariant H347N	Escherichia coli	nicotinamide adenine dinucleotide	4.5	0.073	60	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H347N, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant H347N	Escherichia coli	prephenate	9.8	0.028	350	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant H347N, prephenate
chorismate mutase-prephenate dehydrogenasevariant K37Q	Escherichia coli	nicotinamide adenine dinucleotide	22	0.141	160	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant K37Q, nicotinamide adenine dinucleotide
chorismate mutase-prephenate dehydrogenasevariant K37Q	Escherichia coli	prephenate	23	0.054	430	pH 7.2, 30 °C	Open record: chorismate mutase-prephenate dehydrogenase variant K37Q, prephenate