Values CatLog read from this paper

Probing the catalytic mechanism of prephenate dehydratase by site-directed mutagenesis of the Escherichia coli P-protein dehydratase domain

PMID 10769128doi:10.1021/bi9926680Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (62 values) from this paper: P-protein (bifunctional chorismate mutase/prephenate dehydratase) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.51, EC 5.4.99.5

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
P-protein (bifunctional chorismate mutase/prephenate dehydratase)	Escherichia coli	chorismate	34.18	0.127	268	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase), chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)	Escherichia coli	prephenateP-protein expressed in E. coli NK6024	32.2	0.559	57.7	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase), prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)	Escherichia coli	prephenatePDT20 expressed in E. coli BL21(DE3) from pET26b(+)	31.55	0.648	48.7	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase), prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant C216A	Escherichia coli	chorismate	33.55	0.159	212	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant C216A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant C216A	Escherichia coli	prephenate	—	—	14.8	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant C216A, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant C216S	Escherichia coli	chorismate	32.45	0.113	287	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant C216S, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant C216S	Escherichia coli	prephenate	34.93	2.087	17	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant C216S, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant E159A	Escherichia coli	chorismate	34.22	0.124	275	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant E159A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant E159A	Escherichia coli	prephenate	42.13	0.25	168.5	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant E159A, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant E159A/E232A	Escherichia coli	prephenate	49.37	0.123	400	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant E159A/E232A, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant E232A	Escherichia coli	prephenate	40.02	0.162	247	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant E232A, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant N160A	Escherichia coli	chorismate	33.55	0.151	222	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant N160A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant N160A	Escherichia coli	prephenate	0.22	1.649	0.13	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant N160A, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant Q215A	Escherichia coli	chorismate	35.13	0.154	228	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant Q215A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant Q215A	Escherichia coli	prephenate	—	—	0.15	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant Q215A, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant S208A	Escherichia coli	prephenate	—	—	0.5	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant S208A, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant S208C	Escherichia coli	prephenate	—	—	0.5	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant S208C, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant T278A	Escherichia coli	chorismate	35.52	0.123	288	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant T278A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant T278S	Escherichia coli	chorismate	34.12	0.123	277	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant T278S, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant T278S	Escherichia coli	prephenate	0.41	0.565	0.67	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant T278S, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant T278V	Escherichia coli	chorismate	32.27	0.115	280	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant T278V, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant W226A	Escherichia coli	chorismate	32.6	0.139	235	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant W226A, chorismate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant W226A	Escherichia coli	prephenate	—	—	3	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant W226A, prephenate
P-protein (bifunctional chorismate mutase/prephenate dehydratase)variant W226L	Escherichia coli	prephenate	20.45	3	7.8	37 °C	Open record: P-protein (bifunctional chorismate mutase/prephenate dehydratase) variant W226L, prephenate