EC 4.2.99.8: cysteine synthase

LyasesOpen in Browse

CatLog read 71 records for EC 4.2.99.8 from 6 papers, for 5 organisms. Names in these papers: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform), O-acetylserine sulfhydrylase (OASS), O-acetylserine (thiol) lyase, cysteine synthase A (cytosolic) and 6 more.

EC 4.2.99.8 is no longer current; BRENDA: “transferred to EC 2.5.1.47”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

Papers with records for EC 4.2.99.8
Paper	Organisms	Records
Molecular basis of cysteine biosynthesis in plants: structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana	Arabidopsis thaliana	40
Cysteine synthase (O-acetylserine (thiol) lyase) substrate specificities classify the mitochondrial isoform as a cyanoalanine synthase	Spinacia oleracea	8
Beta-cyanoalanine synthase and cysteine synthase from potato: molecular cloning, biochemical characterization, and spatial and hormonal regulation	Solanum tuberosum	8
Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1	Aeropyrum pernix	6
Interactions between serine acetyltransferase and O-acetylserine (thiol) lyase in higher plants--structural and kinetic properties of the free and bound enzymes	Escherichia coli, Spinacia oleracea	5
Effects of bienzyme complex formation of cysteine synthetase from escherichia coli on some properties and kinetics	Escherichia coli	4

Records

Records for EC 4.2.99.8 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
beta-cyanoalanine synthase PCAS-1 (mitochondrial CAS)	Solanum tuberosum	O-acetyl-L-serine	—	2.77	—	pH 7.5	PMID 11575729	Open record: beta-cyanoalanine synthase PCAS-1 (mitochondrial CAS), O-acetyl-L-serine
beta-cyanoalanine synthase PCAS-1 (mitochondrial CAS)	Solanum tuberosum	sodium sulfide	—	0.746	—	pH 7.5	PMID 11575729	Open record: beta-cyanoalanine synthase PCAS-1 (mitochondrial CAS), sodium sulfide
beta-cyanoalanine synthase PCAS-2	Solanum tuberosum	O-acetyl-L-serine	—	2.14	—	pH 7.5	PMID 11575729	Open record: beta-cyanoalanine synthase PCAS-2, O-acetyl-L-serine
beta-cyanoalanine synthase PCAS-2	Solanum tuberosum	sodium sulfide	—	1.52	—	pH 7.5	PMID 11575729	Open record: beta-cyanoalanine synthase PCAS-2, sodium sulfide
cysteine synthase A (cytosolic)	Spinacia oleracea	chloroalanine	—	1.053	—	pH 8, 26 °C	PMID 10948226	Open record: cysteine synthase A (cytosolic), chloroalanine
cysteine synthase A (cytosolic)	Spinacia oleracea	chloroalanine	—	1.135	—	pH 9.8, 26 °C	PMID 10948226	Open record: cysteine synthase A (cytosolic), chloroalanine
cysteine synthase A (cytosolic)	Spinacia oleracea	cysteine	—	0.108	—	pH 9.8, 26 °C	PMID 10948226	Open record: cysteine synthase A (cytosolic), cysteine
cysteine synthase A (cytosolic)	Spinacia oleracea	O-acetyl-L-serine	—	1.355	—	pH 8, 26 °C	PMID 10948226	Open record: cysteine synthase A (cytosolic), O-acetyl-L-serine
cysteine synthase B (chloroplastic)	Spinacia oleracea	chloroalanine	—	0.785	—	pH 8, 26 °C	PMID 10948226	Open record: cysteine synthase B (chloroplastic), chloroalanine
cysteine synthase B (chloroplastic)	Spinacia oleracea	chloroalanine	—	0.8	—	pH 9.8, 26 °C	PMID 10948226	Open record: cysteine synthase B (chloroplastic), chloroalanine
cysteine synthase B (chloroplastic)	Spinacia oleracea	cysteine	—	0.201	—	pH 9.8, 26 °C	PMID 10948226	Open record: cysteine synthase B (chloroplastic), cysteine
cysteine synthase B (chloroplastic)	Spinacia oleracea	O-acetyl-L-serine	—	1.03	—	pH 8, 26 °C	PMID 10948226	Open record: cysteine synthase B (chloroplastic), O-acetyl-L-serine
cysteine synthase PCS-1 (cytosolic CS)	Solanum tuberosum	O-acetyl-L-serine	—	2.99	—	pH 7.5	PMID 11575729	Open record: cysteine synthase PCS-1 (cytosolic CS), O-acetyl-L-serine
cysteine synthase PCS-1 (cytosolic CS)	Solanum tuberosum	sodium sulfide	—	1.57	—	pH 7.5	PMID 11575729	Open record: cysteine synthase PCS-1 (cytosolic CS), sodium sulfide
cysteine synthase PCS-2 (plastidic CS)	Solanum tuberosum	O-acetyl-L-serine	—	1.83	—	pH 7.5	PMID 11575729	Open record: cysteine synthase PCS-2 (plastidic CS), O-acetyl-L-serine
cysteine synthase PCS-2 (plastidic CS)	Solanum tuberosum	sodium sulfide	—	0.998	—	pH 7.5	PMID 11575729	Open record: cysteine synthase PCS-2 (plastidic CS), sodium sulfide
O-acetylserine (thiol) lyase	Escherichia coli	O-acetyl-L-serine	353	0.64	550	pH 7.5, 25 °C	PMID 9692924	Open record: O-acetylserine (thiol) lyase, O-acetyl-L-serine
O-acetylserine (thiol) lyase	Escherichia coli	O-acetyl-L-serineendogenous E. coli BL21(DE3)pLysS enzyme	88	0.84	105	pH 7.5, 25 °C	PMID 9692924	Open record: O-acetylserine (thiol) lyase, O-acetyl-L-serine
O-acetylserine (thiol) lyase	Escherichia coli	sulfide	—	1.04	102	pH 7.5, 25 °C	PMID 9692924	Open record: O-acetylserine (thiol) lyase, sulfide
O-acetylserine (thiol) lyase	Spinacia oleracea	O-acetyl-L-serinekcat from a Hill fit, n = 1.7	462	—	—	pH 7.5, 25 °C	PMID 9692924	Open record: O-acetylserine (thiol) lyase, O-acetyl-L-serine
O-acetylserine (thiol) lyase	Spinacia oleracea	O-acetyl-L-serineapparent Km	7.1	4.28	1.7	pH 7.5, 25 °C	PMID 9692924	Open record: O-acetylserine (thiol) lyase, O-acetyl-L-serine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)	Arabidopsis thaliana	O-acetylserine	1.78 × 10³	1.4	1.27 × 10³	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform), O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)	Arabidopsis thaliana	sodium sulfide	2.17 × 10³	0.22	9.86 × 10³	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform), sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant H157N	Arabidopsis thaliana	O-acetylserine	1.52 × 10³	0.94	1617	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant H157N, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant H157N	Arabidopsis thaliana	sodium sulfide	1.99 × 10³	0.4	4975	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant H157N, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant H157Q	Arabidopsis thaliana	O-acetylserine	1055	0.91	1.16 × 10³	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant H157Q, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant H157Q	Arabidopsis thaliana	sodium sulfide	1.78 × 10³	0.3	5.93 × 10³	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant H157Q, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant N77A	Arabidopsis thaliana	O-acetylserine	403	4.7	85.7	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant N77A, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant N77A	Arabidopsis thaliana	sodium sulfide	155	0.23	674	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant N77A, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant N77D	Arabidopsis thaliana	O-acetylserine	0.34	10.2	0.033	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant N77D, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant N77D	Arabidopsis thaliana	sodium sulfide	0.73	0.18	4.055	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant N77D, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant Q147A	Arabidopsis thaliana	O-acetylserine	4.02	4.4	0.914	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant Q147A, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant Q147A	Arabidopsis thaliana	sodium sulfide	12.5	1.7	7.35	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant Q147A, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant Q147E	Arabidopsis thaliana	O-acetylserine	0.19	3.8	0.05	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant Q147E, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant Q147E	Arabidopsis thaliana	sodium sulfide	0.22	0.27	0.815	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant Q147E, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S269A	Arabidopsis thaliana	O-acetylserine	34.3	0.57	60.2	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S269A, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S269A	Arabidopsis thaliana	sodium sulfide	29.5	0.54	54.6	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S269A, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S269T	Arabidopsis thaliana	O-acetylserine	572	0.96	596	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S269T, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S269T	Arabidopsis thaliana	sodium sulfide	929	1.6	581	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S269T, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S75A	Arabidopsis thaliana	O-acetylserine	7.16	1.6	4.475	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S75A, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S75A	Arabidopsis thaliana	sodium sulfide	20.2	4.6	4.39	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S75A, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S75N	Arabidopsis thaliana	O-acetylserine	0.09	2	0.045	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S75N, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S75N	Arabidopsis thaliana	sodium sulfide	1.45	5.7	0.254	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S75N, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S75T	Arabidopsis thaliana	O-acetylserine	51.5	1.2	42.9	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S75T, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant S75T	Arabidopsis thaliana	sodium sulfide	82.7	0.21	394	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant S75T, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T182A	Arabidopsis thaliana	O-acetylserine	889	1.2	741	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T182A, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T182A	Arabidopsis thaliana	sodium sulfide	1.6 × 10³	0.39	4 × 10³	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T182A, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T182S	Arabidopsis thaliana	O-acetylserine	1.66 × 10³	1.6	1038	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T182S, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T182S	Arabidopsis thaliana	sodium sulfide	1.69 × 10³	0.22	7.68 × 10³	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T182S, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T185A	Arabidopsis thaliana	O-acetylserine	1.07	1.5	0.713	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T185A, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T185A	Arabidopsis thaliana	sodium sulfide	1.44	0.22	6.55	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T185A, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T185S	Arabidopsis thaliana	O-acetylserine	3.11	1.6	1.94	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T185S, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T185S	Arabidopsis thaliana	sodium sulfide	5.57	0.36	15.5	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T185S, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T74A	Arabidopsis thaliana	O-acetylserine	0.08	1.6	0.05	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T74A, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T74A	Arabidopsis thaliana	sodium sulfide	0.42	3.9	0.108	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T74A, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T74S	Arabidopsis thaliana	O-acetylserine	456	1.5	304	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T74S, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T74S	Arabidopsis thaliana	sodium sulfide	1.7 × 10³	3.2	531	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T74S, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T78A	Arabidopsis thaliana	O-acetylserine	554	0.68	815	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T78A, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T78A	Arabidopsis thaliana	sodium sulfide	537	0.44	1.22 × 10³	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T78A, sodium sulfide
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T78S	Arabidopsis thaliana	O-acetylserine	775	0.82	945	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T78S, O-acetylserine
O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform)variant T78S	Arabidopsis thaliana	sodium sulfide	370	0.3	1.23 × 10³	pH 7, 25 °C	PMID 16166087	Open record: O-acetylserine sulfhydrylase (AtOASS, cytosolic isoform) variant T78S, sodium sulfide
O-acetylserine sulfhydrylase (OASS)	Aeropyrum pernix	L-homocysteine	—	0.51	—	pH 8.3, 85 °C	PMID 12644499	Open record: O-acetylserine sulfhydrylase (OASS), L-homocysteine
O-acetylserine sulfhydrylase (OASS)	Aeropyrum pernix	L-serine	0.7	8	0.087 calc.	pH 8.3, 85 °C	PMID 12644499	Open record: O-acetylserine sulfhydrylase (OASS), L-serine
O-acetylserine sulfhydrylase (OASS)	Aeropyrum pernix	L-serine	0.56	31	0.018 calc.	pH 8.5, 70 °C	PMID 12644499	Open record: O-acetylserine sulfhydrylase (OASS), L-serine
O-acetylserine sulfhydrylase (OASS)	Aeropyrum pernix	O-acetyl-L-serine	202	28	7.2 calc.	pH 6.7, 60 °C	PMID 12644499	Open record: O-acetylserine sulfhydrylase (OASS), O-acetyl-L-serine
O-acetylserine sulfhydrylase (OASS)	Aeropyrum pernix	O-acetyl-L-serine	24	13	1.8 calc.	pH 6.1, 85 °C	PMID 12644499	Open record: O-acetylserine sulfhydrylase (OASS), O-acetyl-L-serine
O-acetylserine sulfhydrylase (OASS)	Aeropyrum pernix	sodium thiosulfate	—	21	—	pH 6.1, 85 °C	PMID 12644499	Open record: O-acetylserine sulfhydrylase (OASS), sodium thiosulfate
O-acetylserine sulfhydrylase-A (OASS-A)	Escherichia coli	O-acetyl-L-serinefree enzyme (no SAT)	2.03 × 10³	4.8	420 calc.	pH 7.5, 25 °C	PMID 10993149	Open record: O-acetylserine sulfhydrylase-A (OASS-A), O-acetyl-L-serine
O-acetylserine sulfhydrylase-A (OASS-A)	Escherichia coli	O-acetyl-L-serineOASS-A bound to wild-type SAT (SAT/OASS-A molar ratio 5)	810	27	30 calc.	pH 7.5, 25 °C	PMID 10993149	Open record: O-acetylserine sulfhydrylase-A (OASS-A), O-acetyl-L-serine
O-acetylserine sulfhydrylase-A (OASS-A)	Escherichia coli	sulfidefree enzyme (no SAT)	—	0.006	—	pH 7.5, 25 °C	PMID 10993149	Open record: O-acetylserine sulfhydrylase-A (OASS-A), sulfide
O-acetylserine sulfhydrylase-A (OASS-A)	Escherichia coli	sulfideOASS-A bound to wild-type SAT (SAT/OASS-A molar ratio 5)	—	0.013	—	pH 7.5, 25 °C	PMID 10993149	Open record: O-acetylserine sulfhydrylase-A (OASS-A), sulfide