Values CatLog read from this paper
Effects of bienzyme complex formation of cysteine synthetase from escherichia coli on some properties and kinetics
PMID 10993149doi:10.1271/bbb.64.1628Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (21 values) from this paper: serine acetyltransferase (SAT), O-acetylserine sulfhydrylase-A (OASS-A) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| O-acetylserine sulfhydrylase-A (OASS-A) | Escherichia coli | O-acetyl-L-serinefree enzyme (no SAT) | 2.03 × 10³ | 4.8 | 420 calc. | pH 7.5, 25 °C | Open record: O-acetylserine sulfhydrylase-A (OASS-A), O-acetyl-L-serine |
| O-acetylserine sulfhydrylase-A (OASS-A) | Escherichia coli | O-acetyl-L-serineOASS-A bound to wild-type SAT (SAT/OASS-A molar ratio 5) | 810 | 27 | 30 calc. | pH 7.5, 25 °C | Open record: O-acetylserine sulfhydrylase-A (OASS-A), O-acetyl-L-serine |
| O-acetylserine sulfhydrylase-A (OASS-A) | Escherichia coli | sulfidefree enzyme (no SAT) | — | 0.006 | — | pH 7.5, 25 °C | Open record: O-acetylserine sulfhydrylase-A (OASS-A), sulfide |
| O-acetylserine sulfhydrylase-A (OASS-A) | Escherichia coli | sulfideOASS-A bound to wild-type SAT (SAT/OASS-A molar ratio 5) | — | 0.013 | — | pH 7.5, 25 °C | Open record: O-acetylserine sulfhydrylase-A (OASS-A), sulfide |
| serine acetyltransferase (SAT) | Escherichia coli | acetyl coenzyme Afree enzyme (no OASS-A) | — | 0.58 | — | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT), acetyl coenzyme A |
| serine acetyltransferase (SAT) | Escherichia coli | acetyl coenzyme A | — | 0.51 | — | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT), acetyl coenzyme A |
| serine acetyltransferase (SAT) | Escherichia coli | coenzyme A | 1.1 × 10³ | 0.69 | 1.6 × 10³ calc. | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT), coenzyme A |
| serine acetyltransferase (SAT) | Escherichia coli | L-serinefree enzyme (no OASS-A) | 2.48 × 10³ | 1.9 | 1.3 × 10³ calc. | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT), L-serine |
| serine acetyltransferase (SAT) | Escherichia coli | L-serine | 2.48 × 10³ | 2.3 | 1.1 × 10³ calc. | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT), L-serine |
| serine acetyltransferase (SAT) | Escherichia coli | O-acetyl-L-serine | — | 7.3 | — | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT), O-acetyl-L-serine |
| serine acetyltransferase (SAT)variant ΔC20 | Escherichia coli | acetyl coenzyme A | — | 0.63 | — | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT) variant ΔC20, acetyl coenzyme A |
| serine acetyltransferase (SAT)variant ΔC20 | Escherichia coli | coenzyme A | 1.18 × 10³ | 1.1 | 1.1 × 10³ calc. | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT) variant ΔC20, coenzyme A |
| serine acetyltransferase (SAT)variant ΔC20 | Escherichia coli | L-serine | 2.48 × 10³ | 2 | 1.2 × 10³ calc. | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT) variant ΔC20, L-serine |
| serine acetyltransferase (SAT)variant ΔC20 | Escherichia coli | O-acetyl-L-serine | — | 7.4 | — | pH 7.5, 25 °C | Open record: serine acetyltransferase (SAT) variant ΔC20, O-acetyl-L-serine |