CatLogenzyme kinetics catalog

EC 4.3.1.1: aspartate ammonia-lyase

LyasesOpen in Browse

CatLog read 21 records for EC 4.3.1.1 from 5 papers, for 7 organisms. Names in these papers: L-aspartase, aspartate ammonia lyase (AspB), L-aspartate ammonia lyase (aspartase, PA-AspA), AspB (aspartate ammonia-lyase, aspartase) and 4 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 61 records for EC 4.3.1.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.3.1.1 in Browse.

Papers

PaperOrganismsRecords
Bioinformatics and Computationally Supported Redesign of Aspartase for β-Alanine Synthesis by Acrylic Acid HydroaminationBacillus sp. YM55-1, Brevibacillus borstelensis and 2 more8
Mutagenic investigation of conserved functional amino acids in Escherichia coli L-aspartaseEscherichia coli5
Structural basis for the catalytic mechanism of aspartate ammonia lyaseBacillus sp. YM55-14
Bioproduction of L-Aspartic Acid and Cinnamic Acid by L-Aspartate Ammonia Lyase from Pseudomonas aeruginosa PAO1Pseudomonas aeruginosa3
Enhanced Stability of Lactobacillus paracasei Aspartate Ammonia-Lyase via Electrospinning for Enzyme ImmobilizationLactobacillus paracasei1

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
aspartate ammonia lyase (AspB)variant I320ABacillus sp. YM55-1L-aspartic acid——0.055pH 8.5, 25 °CPMID 21661762Open
aspartate ammonia lyase (AspB)variant M321ABacillus sp. YM55-1L-aspartic acid——0.008pH 8.5, 25 °CPMID 21661762Open
aspartate ammonia lyase (AspB)variant P322ABacillus sp. YM55-1L-aspartic acid21111.9pH 8.5, 25 °CPMID 21661762Open
aspartate ammonia lyase (AspB)variant S319ABacillus sp. YM55-1L-aspartic acid17280.61pH 8.5, 25 °CPMID 21661762Open
aspartate ammonia-lyase (LpAAL)Lactobacillus paracaseiL-aspartic acid7.36.31.2pH 8, 40 °CPMID 39940473Open
AspB (aspartate ammonia-lyase, aspartase)variant T187I/M321I/K324M/N326C (AspB-A1)Bacillus sp. YM55-1acrylic acid——5.3 × 10⁻⁴pH 9, 37 °CPMID 39839848Open
AspB (aspartate ammonia-lyase, aspartase)variant T187I/M321I/K324M/N326C/A422S/Y429D (AspB-6x)Bacillus sp. YM55-1acrylic acid——0.0019pH 9, 37 °CPMID 39839848Open
BbAsp (aspartase)variant A1 carboxylate pocket (positions corresponding to AspB T187I/M321I/K324M/N326C) + A424E (BbAsp-5x)Brevibacillus borstelensisacrylic acid——0.0042pH 9, 37 °CPMID 39839848Open
BbAsp (aspartase)variant A1 carboxylate pocket, at the positions corresponding to AspB T187I/M321I/K324M/N326C (BbAsp-A1)Brevibacillus borstelensisacrylic acid——0.0016pH 9, 37 °CPMID 39839848Open
CcAsp (aspartase)variant A1 carboxylate pocket (positions corresponding to AspB T187I/M321I/K324M/N326C) + A424S/I431T (CcAsp-6x)Caenibacillus caldisaponilyticusacrylic acid0.742300.0032pH 9, 37 °CPMID 39839848Open
CcAsp (aspartase)variant A1 carboxylate pocket, at the positions corresponding to AspB T187I/M321I/K324M/N326C (CcAsp-A1)Caenibacillus caldisaponilyticusacrylic acid0.491800.0027pH 9, 37 °CPMID 39839848Open
L-aspartaseEscherichia coliL-aspartate1.0234.30.24pH 7, 30 °CPMID 8119980Open
L-aspartasevariant C389AEscherichia coliL-aspartate1.1035.20.212pH 7, 30 °CPMID 8119980Open
L-aspartasevariant C389SEscherichia coliL-aspartate0.80330.267pH 7, 30 °CPMID 8119980Open
L-aspartasevariant H123LEscherichia coliL-aspartate0.8452.10.398pH 7, 30 °CPMID 8119980Open
L-aspartasevariant K326REscherichia coliL-aspartate0.003323.81.5 × 10⁻⁴pH 7, 30 °CPMID 8119980Open
L-aspartate ammonia lyase (aspartase, PA-AspA)Pseudomonas aeruginosaL-aspartic acid4.9083.46 × 10⁻⁴1.418 × 10⁴pH 8, 35 °CPMID 27988856Open
L-aspartate ammonia lyase (aspartase, PA-AspA)Pseudomonas aeruginosaL-phenylalanine8.0021.7194.65pH 8, 35 °CPMID 27988856Open
L-aspartate ammonia lyase (aspartase, PA-AspA)Pseudomonas aeruginosasodium fumarate13.64—0.038pH 8, 35 °CPMID 27988856Open
StAsp (aspartase)variant A1 carboxylate pocket (positions corresponding to AspB T187I/M321I/K324M/N326C) + S421E (StAsp-5x)Symbiobacterium thermophilumacrylic acid——0.0033pH 9, 37 °CPMID 39839848Open
StAsp (aspartase)variant A1 carboxylate pocket, at the positions corresponding to AspB T187I/M321I/K324M/N326C (StAsp-A1)Symbiobacterium thermophilumacrylic acid——6.6 × 10⁻⁴pH 9, 37 °CPMID 39839848Open