Values CatLog read from this paper
Bioinformatics and Computationally Supported Redesign of Aspartase for β-Alanine Synthesis by Acrylic Acid Hydroamination
PMID 39839848doi:10.1021/acscatal.4c05525Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (12 values) from this paper: AspB (aspartate ammonia-lyase, aspartase), BbAsp (aspartase), CcAsp (aspartase) and 1 more from Bacillus sp. YM55-1, Brevibacillus borstelensis, Caenibacillus caldisaponilyticus and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| AspB (aspartate ammonia-lyase, aspartase)variant T187I/M321I/K324M/N326C (AspB-A1) | Bacillus sp. YM55-1 | acrylic acid | — | — | 5.3 × 10⁻⁴ | pH 9, 37 °C | Open record: AspB (aspartate ammonia-lyase, aspartase) variant T187I/M321I/K324M/N326C (AspB-A1), acrylic acid |
| AspB (aspartate ammonia-lyase, aspartase)variant T187I/M321I/K324M/N326C/A422S/Y429D (AspB-6x) | Bacillus sp. YM55-1 | acrylic acid | — | — | 0.0019 | pH 9, 37 °C | Open record: AspB (aspartate ammonia-lyase, aspartase) variant T187I/M321I/K324M/N326C/A422S/Y429D (AspB-6x), acrylic acid |
| BbAsp (aspartase)variant A1 carboxylate pocket (positions corresponding to AspB T187I/M321I/K324M/N326C) + A424E (BbAsp-5x) | Brevibacillus borstelensis | acrylic acid | — | — | 0.0042 | pH 9, 37 °C | Open record: BbAsp (aspartase) variant A1 carboxylate pocket (positions corresponding to AspB T187I/M321I/K324M/N326C) + A424E (BbAsp-5x), acrylic acid |
| BbAsp (aspartase)variant A1 carboxylate pocket, at the positions corresponding to AspB T187I/M321I/K324M/N326C (BbAsp-A1) | Brevibacillus borstelensis | acrylic acid | — | — | 0.0016 | pH 9, 37 °C | Open record: BbAsp (aspartase) variant A1 carboxylate pocket, at the positions corresponding to AspB T187I/M321I/K324M/N326C (BbAsp-A1), acrylic acid |
| CcAsp (aspartase)variant A1 carboxylate pocket (positions corresponding to AspB T187I/M321I/K324M/N326C) + A424S/I431T (CcAsp-6x) | Caenibacillus caldisaponilyticus | acrylic acid | 0.74 | 230 | 0.0032 | pH 9, 37 °C | Open record: CcAsp (aspartase) variant A1 carboxylate pocket (positions corresponding to AspB T187I/M321I/K324M/N326C) + A424S/I431T (CcAsp-6x), acrylic acid |
| CcAsp (aspartase)variant A1 carboxylate pocket, at the positions corresponding to AspB T187I/M321I/K324M/N326C (CcAsp-A1) | Caenibacillus caldisaponilyticus | acrylic acid | 0.49 | 180 | 0.0027 | pH 9, 37 °C | Open record: CcAsp (aspartase) variant A1 carboxylate pocket, at the positions corresponding to AspB T187I/M321I/K324M/N326C (CcAsp-A1), acrylic acid |
| StAsp (aspartase)variant A1 carboxylate pocket (positions corresponding to AspB T187I/M321I/K324M/N326C) + S421E (StAsp-5x) | Symbiobacterium thermophilum | acrylic acid | — | — | 0.0033 | pH 9, 37 °C | Open record: StAsp (aspartase) variant A1 carboxylate pocket (positions corresponding to AspB T187I/M321I/K324M/N326C) + S421E (StAsp-5x), acrylic acid |
| StAsp (aspartase)variant A1 carboxylate pocket, at the positions corresponding to AspB T187I/M321I/K324M/N326C (StAsp-A1) | Symbiobacterium thermophilum | acrylic acid | — | — | 6.6 × 10⁻⁴ | pH 9, 37 °C | Open record: StAsp (aspartase) variant A1 carboxylate pocket, at the positions corresponding to AspB T187I/M321I/K324M/N326C (StAsp-A1), acrylic acid |