CatLogenzyme kinetics catalog

EC 4.4.1.5: lactoylglutathione lyase

LyasesOpen in Browse

CatLog read 59 records for EC 4.4.1.5 from 9 papers, for 11 organisms. Names in these papers: glyoxalase I, glyoxalase-I, glyoxalase I (GlxI), glyoxalase I (HsGLO1) and 4 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 79 records for EC 4.4.1.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.4.1.5 in Browse.

Papers

PaperOrganismsRecords
Purification and kinetic study of glyoxalase-I from rat liver, erythrocytes, brain and kidneyRattus norvegicus11
Specificity of the trypanothione-dependent Leishmania major glyoxalase I: structure and biochemical comparison with the human enzymeHomo sapiens, Leishmania major10
Comparison of glyoxalase I purified from yeast (Saccharomyces cerevisiae) with the enzyme from mammalian sourcesHomo sapiens, Rattus norvegicus and 2 more8
Yeast glyoxalase I is a monomeric enzyme with two active sitesSaccharomyces cerevisiae7
Mutagenesis of residue 157 in the active site of human glyoxalase IHomo sapiens7
Distinct classes of glyoxalase I: metal specificity of the Yersinia pestis, Pseudomonas aeruginosa and Neisseria meningitidis enzymesNeisseria meningitidis, Pseudomonas aeruginosa and 1 more6
Investigation of metal binding and activation of Escherichia coli glyoxalase I: kinetic, thermodynamic and mutagenesis studiesEscherichia coli4
Identification of thermostable glyoxalase I in the fission yeast Schizosaccharomyces pombeSchizosaccharomyces pombe4
Characterization of the glyoxalases of the malarial parasite Plasmodium falciparum and comparison with their human counterpartsHomo sapiens, Plasmodium falciparum2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
cGloI (cytosolic glyoxalase I)Plasmodium falciparummethylglyoxal-glutathione hemithioacetal110.60.0284 × 10³pH 7, 30 °CPMID 15843146Open
glyoxalase IHomo sapienshemithioacetal of GSH and methylglyoxal1.5 × 10³0.0662.3 × 10⁴pH 7, 30 °CPMID 9359858Open
glyoxalase Ivariant M157AHomo sapienshemithioacetal of GSH and methylglyoxal2600.0744 × 10³pH 7, 30 °CPMID 9359858Open
glyoxalase Ivariant M157AHomo sapienshemithioacetal of GSH and phenylglyoxal——1 × 10⁴pH 7, 30 °CPMID 9359858Open
glyoxalase Ivariant M157HHomo sapienshemithioacetal of GSH and methylglyoxal3000.0893 × 10³pH 7, 30 °CPMID 9359858Open
glyoxalase Ivariant M157HHomo sapienshemithioacetal of GSH and phenylglyoxal——1 × 10⁴pH 7, 30 °CPMID 9359858Open
glyoxalase Ivariant M157QHomo sapienshemithioacetal of GSH and methylglyoxal1.65 × 10³0.1621 × 10⁴pH 7, 30 °CPMID 9359858Open
glyoxalase Ivariant M157QHomo sapienshemithioacetal of GSH and phenylglyoxal——4.4 × 10⁴pH 7, 30 °CPMID 9359858Open
glyoxalase IRattus norvegicusMethylglyoxal1.18 × 10³——pH 7, 30 °CPMID 393249Open
glyoxalase IRattus norvegicusPhenylglyoxal1.1 × 10³——pH 7, 30 °CPMID 393249Open
glyoxalase ISaccharomyces cerevisiaehemimercaptal adduct of methylglyoxal and glutathionewithout addition of metals to the growth medium1.7 × 10³0.414.2 × 10³pH 7, 30 °CPMID 11050082Open
glyoxalase ISaccharomyces cerevisiaehemimercaptal adduct of methylglyoxal and glutathione1.1 × 10³0.372.9 × 10³pH 7, 30 °CPMID 11050082Open
glyoxalase ISaccharomyces cerevisiaehemimercaptal adduct of methylglyoxal and glutathionewith addition of 1 mM Mn(II) to the growth medium1.6 × 10³0.453.5 × 10³pH 7, 30 °CPMID 11050082Open
glyoxalase ISaccharomyces cerevisiaehemimercaptal adduct of methylglyoxal and glutathionewith addition of 1 mM Co(II) to the growth medium2.7 × 10³0.241.1 × 10⁴pH 7, 30 °CPMID 11050082Open
glyoxalase ISaccharomyces cerevisiaeMethylglyoxal1.82 × 10³——pH 7, 30 °CPMID 393249Open
glyoxalase ISaccharomyces cerevisiaePhenylglyoxal6.7 × 10³——pH 7, 30 °CPMID 393249Open
glyoxalase Ivariant E163QSaccharomyces cerevisiaehemimercaptal adduct of methylglyoxal and glutathione9900.33.3 × 10³pH 7, 30 °CPMID 11050082Open
glyoxalase Ivariant E163Q/E318QSaccharomyces cerevisiaehemimercaptal adduct of methylglyoxal and glutathione0.310.142.3pH 7, 30 °CPMID 11050082Open
glyoxalase Ivariant E318QSaccharomyces cerevisiaehemimercaptal adduct of methylglyoxal and glutathione2800.241.2 × 10³pH 7, 30 °CPMID 11050082Open
glyoxalase ISchizosaccharomyces pombeglutathione—0.8—pH 7, 25 °CPMID 15042280Open
glyoxalase ISchizosaccharomyces pombemethylglyoxal11471.6720 calc.pH 7, 25 °CPMID 15042280Open
glyoxalase ISchizosaccharomyces pombemethylglyoxal——720pH 7, 25 °CPMID 15042280Open
glyoxalase ISchizosaccharomyces pombephenylglyoxal111.30.24460 calc.pH 7, 25 °CPMID 15042280Open
glyoxalase ISus scrofaMethylglyoxal980——pH 7, 30 °CPMID 393249Open
glyoxalase ISus scrofaPhenylglyoxal620——pH 7, 30 °CPMID 393249Open
glyoxalase I (GlxI)Escherichia colihemithioacetal formed between methylglyoxal and glutathione50 mM potassium phosphate (standard assay buffer)1060.0128.8 × 10³pH 6.6PMID 14556652Open
glyoxalase I (GlxI)Escherichia colihemithioacetal formed between methylglyoxal and glutathioneMes buffer (instead of phosphate)60.20.016 × 10³pH 6.6PMID 14556652Open
glyoxalase I (GlxI)Escherichia colihemithioacetal formed between methylglyoxal and glutathione50 mM potassium phosphate (standard assay buffer)55.70.015.6 × 10³pH 6.6PMID 14556652Open
glyoxalase I (GlxI)Escherichia colihemithioacetal formed between methylglyoxal and glutathione50 mM potassium phosphate (standard assay buffer)21.40.00892.4 × 10³pH 6.6PMID 14556652Open
glyoxalase I (GlxI)Neisseria meningitidismethylglyoxal-glutathione hemithioacetal2040.0454.5 × 10³PMID 15270717Open
glyoxalase I (GlxI)Neisseria meningitidismethylglyoxal-glutathione hemithioacetal1460.0285.2 × 10³PMID 15270717Open
glyoxalase I (GlxI)Pseudomonas aeruginosamethylglyoxal-glutathione hemithioacetal2710.0328.5 × 10³PMID 15270717Open
glyoxalase I (GlxI)Pseudomonas aeruginosamethylglyoxal-glutathione hemithioacetal860.0165.4 × 10³PMID 15270717Open
glyoxalase I (GlxI)Yersinia pestismethylglyoxal-glutathione hemithioacetal3060.0565.5 × 10³PMID 15270717Open
glyoxalase I (GlxI)Yersinia pestismethylglyoxal-glutathione hemithioacetal690.0292.4 × 10³PMID 15270717Open
glyoxalase I (HsGLO1)Homo sapiensglutathione ethyl ester methylglyoxal hemithioacetal1640.0423.9 × 10³pH 7, 27 °CPMID 16430697Open
glyoxalase I (HsGLO1)Homo sapiensglutathione isopropyl ester methylglyoxal hemithioacetal2220.0534.2 × 10³pH 7, 27 °CPMID 16430697Open
glyoxalase I (HsGLO1)Homo sapiensglutathione methylglyoxal hemithioacetal1.36 × 10³0.0492.8 × 10⁴pH 7, 27 °CPMID 16430697Open
glyoxalase I (HsGLO1)Homo sapiensN1-glutathionylspermidine methylglyoxal hemithioacetal830.148600pH 7, 27 °CPMID 16430697Open
glyoxalase I (HsGLO1)Homo sapienstrypanothione (N1,N8-bis(glutathionyl)spermidine) methylglyoxal hemithioacetal1040.13800pH 7, 27 °CPMID 16430697Open
glyoxalase I (LmGLO1, trypanothione-dependent, Ni2+-dependent)Leishmania majorglutathione ethyl ester methylglyoxal hemithioacetal——900pH 7, 27 °CPMID 16430697Open
glyoxalase I (LmGLO1, trypanothione-dependent, Ni2+-dependent)Leishmania majorglutathione isopropyl ester methylglyoxal hemithioacetal1.17 × 10³0.33.9 × 10³pH 7, 27 °CPMID 16430697Open
glyoxalase I (LmGLO1, trypanothione-dependent, Ni2+-dependent)Leishmania majorglutathione methylglyoxal hemithioacetal——90pH 7, 27 °CPMID 16430697Open
glyoxalase I (LmGLO1, trypanothione-dependent, Ni2+-dependent)Leishmania majorN1-glutathionylspermidine methylglyoxal hemithioacetal1.59 × 10³0.0642.5 × 10⁴pH 7, 27 °CPMID 16430697Open
glyoxalase I (LmGLO1, trypanothione-dependent, Ni2+-dependent)Leishmania majortrypanothione (N1,N8-bis(glutathionyl)spermidine) methylglyoxal hemithioacetal1.07 × 10³0.0711.5 × 10⁴pH 7, 27 °CPMID 16430697Open
glyoxalase I form 1Homo sapiensMethylglyoxal1.13 × 10³——pH 7, 30 °CPMID 393249Open
glyoxalase I form 1Homo sapiensPhenylglyoxal1.07 × 10³——pH 7, 30 °CPMID 393249Open
glyoxalase-IRattus norvegicus2,4-Dimethylphenylglyoxal—0.07—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusm-Methoxyphenylglyoxal—0.03—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusMethylglyoxal—0.09—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusp-Bromophenylglyoxal—0.02—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusp-Chlorophenylglyoxal—0.02—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusp-Hydroxyphenylglyoxal—0.03—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusp-Methoxyphenylglyoxal—0.01—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusp-Methylphenylglyoxal—0.02—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusp-Phenylphenylglyoxal—0.02—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusPhenylglyoxal—0.04—pH 7, 25 °CPMID 953039Open
glyoxalase-IRattus norvegicusβ-ethoxy-α-ketobutyraldehyde—0.2—pH 7, 25 °CPMID 953039Open
human glyoxalase I (Human GloI)Homo sapiensmethylglyoxal-glutathione hemithioacetal39.580.09440pH 7, 30 °CPMID 15843146Open