EC 4.6.1.1: adenylate cyclase
LyasesOpen in Browse
CatLog read 58 records for EC 4.6.1.1 from 2 papers, for 2 organisms. Names in these papers: class IV adenylyl cyclase (YpAC-IV), adenylate cyclase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 138 records for EC 4.6.1.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.6.1.1 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Active-site structure of class IV adenylyl cyclase and transphyletic mechanism | Yersinia pestis | 43 |
| Structure-function relationships in Escherichia coli adenylate cyclase | Escherichia coli | 15 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| adenylate cyclase | Escherichia coli | ATP | — | 0.27 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase, ATP |
| adenylate cyclasevariant E185A | Escherichia coli | ATP | — | 0.275 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant E185A, ATP |
| adenylate cyclasevariant E242A | Escherichia coli | ATP | — | 0.216 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant E242A, ATP |
| adenylate cyclasevariant K136A | Escherichia coli | ATP | — | 0.201 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant K136A, ATP |
| adenylate cyclasevariant K253A | Escherichia coli | ATP | — | 0.259 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant K253A, ATP |
| adenylate cyclasevariant K260A | Escherichia coli | ATP | — | 0.284 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant K260A, ATP |
| adenylate cyclasevariant K332A | Escherichia coli | ATP | — | 0.682 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant K332A, ATP |
| adenylate cyclasevariant R19A | Escherichia coli | ATP | — | 0.46 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant R19A, ATP |
| adenylate cyclasevariant S103A | Escherichia coli | ATP | — | 4.7 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant S103A, ATP |
| adenylate cyclasevariant S106A | Escherichia coli | ATP | — | 0.533 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant S106A, ATP |
| adenylate cyclasevariant S113A | Escherichia coli | ATP | — | 0.33 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant S113A, ATP |
| adenylate cyclasevariant T189A | Escherichia coli | ATP | — | 0.398 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant T189A, ATP |
| adenylate cyclasevariant W249A | Escherichia coli | ATP | — | 0.168 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant W249A, ATP |
| adenylate cyclasevariant W374A | Escherichia coli | ATP | — | 0.165 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant W374A, ATP |
| adenylate cyclasevariant Y394A | Escherichia coli | ATP | — | 0.153 | — | pH 8, 37 °C | PMID 18620542 | Open record: adenylate cyclase variant Y394A, ATP |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphate50 mM Tris-HCl; 20 mM MgCl2; 1 mM DTT; [alpha-32P]ATP | — | 0.78 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphate50 mM Tris-HCl; 10 mM MnCl2; 1 mM DTT; [alpha-32P]ATP | — | 0.02 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT | 0.0057 | 0.21 | 0.027 calc. | pH 7, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT | 0.0349 | 0.37 | 0.094 calc. | pH 7.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT | 0.1002 | 0.34 | 0.29 calc. | pH 8, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT | 0.2519 | 0.43 | 0.59 calc. | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT | 0.4943 | 0.44 | 1.1 calc. | pH 9, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT | 0.7449 | 0.69 | 1.1 calc. | pH 9.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT | 0.8368 | 0.7 | 1.2 calc. | pH 10, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT | 0.889 | 0.71 | 1.3 calc. | pH 10.4, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min | — | — | 0.027 | pH 7, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min | — | — | 0.094 | pH 7.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min | — | — | 0.294 | pH 8, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min | — | — | 0.586 | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min | — | — | 1.124 | pH 9, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min | — | — | 1.079 | pH 9.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min | — | — | 1.195 | pH 10, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | adenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min | — | — | 1.252 | pH 10.4, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | guanosine 5'-triphosphateguanylyl cyclase assay: 50 mM Tris-Cl; 1 mM [alpha-32P]GTP | — | 1.38 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), guanosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV) | Yersinia pestis | guanosine 5'-triphosphate50 mM Tris-Cl; GC assay [alpha-32P]GTP -> cGMP | — | — | 0.105 | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV), guanosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant C83A | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 0.62 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant C83A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant C83A | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.005 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant C83A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant D55K | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 0.87 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant D55K, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant D55K | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.014 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant D55K, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant E10Q | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 0.24 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant E10Q, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant E10Q | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.1 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant E10Q, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant E12Q | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 1.15 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant E12Q, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant E12Q | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.015 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant E12Q, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant E136A | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 0.16 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant E136A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant E136A | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.06 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant E136A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant F5A | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 1.89 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant F5A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant F5A | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.03 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant F5A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant K14A | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 0.21 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant K14A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant K14A | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.09 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant K14A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant K76A | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 0.4 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant K76A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant K76A | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.046 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant K76A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant L72A | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 2.29 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant L72A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant L72A | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.035 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant L72A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant M140A | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 1.19 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant M140A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant M140A | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.035 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant M140A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant R113A | Yersinia pestis | adenosine 5'-triphosphate | — | 0.05 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant R113A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant R63A | Yersinia pestis | adenosine 5'-triphosphate20 mM MgCl2 | — | 0.75 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant R63A, adenosine 5'-triphosphate |
| class IV adenylyl cyclase (YpAC-IV)variant R63A | Yersinia pestis | adenosine 5'-triphosphate10 mM MnCl2 | — | 0.011 | — | pH 8.5, 37 °C | PMID 21094652 | Open record: class IV adenylyl cyclase (YpAC-IV) variant R63A, adenosine 5'-triphosphate |