CatLogenzyme kinetics catalog

Values CatLog read from this paper

Active-site structure of class IV adenylyl cyclase and transphyletic mechanism

PMID 21094652doi:10.1016/j.jmb.2010.11.026Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 43 records (51 values) from this paper: class IV adenylyl cyclase (YpAC-IV) from Yersinia pestis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 4.6.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphate50 mM Tris-HCl; 20 mM MgCl2; 1 mM DTT; [alpha-32P]ATP—0.78—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphate50 mM Tris-HCl; 10 mM MnCl2; 1 mM DTT; [alpha-32P]ATP—0.02—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT0.00570.210.027 calc.pH 7, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT0.03490.370.094 calc.pH 7.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT0.10020.340.29 calc.pH 8, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT0.25190.430.59 calc.pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT0.49430.441.1 calc.pH 9, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT0.74490.691.1 calc.pH 9.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT0.83680.71.2 calc.pH 10, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer with Mg (standard assay 20 mM MgCl2); 1 mM DTT0.8890.711.3 calc.pH 10.4, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min——0.027pH 7, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min——0.094pH 7.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min——0.294pH 8, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min——0.586pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min——1.124pH 9, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min——1.079pH 9.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min——1.195pH 10, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisadenosine 5'-triphosphateTris buffer; radiometric ATP -> cAMP assay; 5 min——1.252pH 10.4, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisguanosine 5'-triphosphateguanylyl cyclase assay: 50 mM Tris-Cl; 1 mM [alpha-32P]GTP—1.38—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)Yersinia pestisguanosine 5'-triphosphate50 mM Tris-Cl; GC assay [alpha-32P]GTP -> cGMP——0.105pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant C83AYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—0.62—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant C83AYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.005—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant D55KYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—0.87—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant D55KYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.014—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant E10QYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—0.24—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant E10QYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.1—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant E12QYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—1.15—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant E12QYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.015—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant E136AYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—0.16—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant E136AYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.06—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant F5AYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—1.89—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant F5AYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.03—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant K14AYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—0.21—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant K14AYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.09—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant K76AYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—0.4—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant K76AYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.046—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant L72AYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—2.29—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant L72AYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.035—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant M140AYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—1.19—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant M140AYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.035—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant R113AYersinia pestisadenosine 5'-triphosphate—0.05—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant R63AYersinia pestisadenosine 5'-triphosphate20 mM MgCl2—0.75—pH 8.5, 37 °COpen
class IV adenylyl cyclase (YpAC-IV)variant R63AYersinia pestisadenosine 5'-triphosphate10 mM MnCl2—0.011—pH 8.5, 37 °COpen