CatLogenzyme kinetics catalog

EC 4.99.1.1: protoporphyrin ferrochelatase

LyasesOpen in Browse

CatLog read 28 records for EC 4.99.1.1 from 2 papers, for 1 organism. Names in these papers: ferrochelatase.

EC 4.99.1.1 is no longer current; BRENDA: “deleted. Now classified as EC 4.98.1.1”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 27 records for EC 4.99.1.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.99.1.1 in Browse.

Papers

PaperOrganismsRecords
Nickel(II) chelatase variants directly evolved from murine ferrochelatase: porphyrin distortion and kinetic mechanismMus musculus16
Probing the active site loop motif of murine ferrochelatase by random mutagenesisMus musculus12

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
ferrochelataseMus musculusFe2+—0.0022662.5pH 8, 30 °CPMID 21222436Open
ferrochelataseMus musculusiron(2+)—0.001936pH 8.1, 30 °CPMID 14981080Open
ferrochelataseMus musculusNi2+—0.022060.557pH 8, 30 °CPMID 21222436Open
ferrochelataseMus musculusprotoporphyrin IX0.0680.001448.8pH 8.1, 30 °CPMID 14981080Open
ferrochelataseMus musculusprotoporphyrin IXaerobic; Ni2+ below 40 µM (no substrate inhibition)0.01252.6 × 10⁻⁴47.3pH 8, 30 °CPMID 21222436Open
ferrochelataseMus musculusprotoporphyrin IXanaerobic; cosubstrate Fe2+0.1420.0017282.3pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant F323LMus musculusFe2+—0.00895179pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant F323LMus musculusNi2+—0.032181.605pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant F323LMus musculusprotoporphyrin IXaerobic; Ni2+ below 40 µM (no substrate inhibition)0.2120.00123173pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant F323LMus musculusprotoporphyrin IXanaerobic; cosubstrate Fe2+1.6030.00547292.8pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant K250M/V251L/W256YMus musculusiron(2+)—0.0010846.3pH 8.1, 30 °CPMID 14981080Open
ferrochelatasevariant K250M/V251L/W256YMus musculusprotoporphyrin IX0.050.011254.5pH 8.1, 30 °CPMID 14981080Open
ferrochelatasevariant P255RMus musculusiron(2+)—0.0014689pH 8.1, 30 °CPMID 14981080Open
ferrochelatasevariant P255RMus musculusprotoporphyrin IX0.130.0026549pH 8.1, 30 °CPMID 14981080Open
ferrochelatasevariant Q248P/S249G/K250P/G252WMus musculusiron(2+)—4.8 × 10⁻⁴490pH 8.1, 30 °CPMID 14981080Open
ferrochelatasevariant Q248P/S249G/K250P/G252WMus musculusprotoporphyrin IX0.2350.0103422.7pH 8.1, 30 °CPMID 14981080Open
ferrochelatasevariant S143TMus musculusFe2+—0.00291416.8pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant S143TMus musculusNi2+—0.065219.05pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant S143TMus musculusprotoporphyrin IXaerobic; Ni2+ below 40 µM (no substrate inhibition)0.592.9 × 10⁻⁴2015pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant S143TMus musculusprotoporphyrin IXanaerobic; cosubstrate Fe2+1.2120.00831145.7pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant S143T/F323LMus musculusFe2+—0.00728261.3pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant S143T/F323LMus musculusNi2+—0.10374.23pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant S143T/F323LMus musculusprotoporphyrin IXaerobic; Ni2+ below 40 µM (no substrate inhibition)0.4380.00243180pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant S143T/F323LMus musculusprotoporphyrin IXanaerobic; cosubstrate Fe2+1.9030.00558340.5pH 8, 30 °CPMID 21222436Open
ferrochelatasevariant S249A/K250Q/V251CMus musculusiron(2+)—0.0051558.3pH 8.1, 30 °CPMID 14981080Open
ferrochelatasevariant S249A/K250Q/V251CMus musculusprotoporphyrin IX0.30.0068443.8pH 8.1, 30 °CPMID 14981080Open
ferrochelatasevariant V251LMus musculusiron(2+)—0.00113122.5pH 8.1, 30 °CPMID 14981080Open
ferrochelatasevariant V251LMus musculusprotoporphyrin IX0.1380.0018276pH 8.1, 30 °CPMID 14981080Open