Values CatLog read from this paper
Nickel(II) chelatase variants directly evolved from murine ferrochelatase: porphyrin distortion and kinetic mechanism
PMID 21222436doi:10.1021/bi101170pOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (40 values) from this paper: ferrochelatase from Mus musculus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ferrochelatase | Mus musculus | Fe2+ | — | 0.00226 | 62.5 | pH 8, 30 °C | Open record: ferrochelatase, Fe2+ |
| ferrochelatase | Mus musculus | Ni2+ | — | 0.02206 | 0.557 | pH 8, 30 °C | Open record: ferrochelatase, Ni2+ |
| ferrochelatase | Mus musculus | protoporphyrin IXaerobic; Ni2+ below 40 µM (no substrate inhibition) | 0.0125 | 2.6 × 10⁻⁴ | 47.3 | pH 8, 30 °C | Open record: ferrochelatase, protoporphyrin IX |
| ferrochelatase | Mus musculus | protoporphyrin IXanaerobic; cosubstrate Fe2+ | 0.142 | 0.00172 | 82.3 | pH 8, 30 °C | Open record: ferrochelatase, protoporphyrin IX |
| ferrochelatasevariant F323L | Mus musculus | Fe2+ | — | 0.00895 | 179 | pH 8, 30 °C | Open record: ferrochelatase variant F323L, Fe2+ |
| ferrochelatasevariant F323L | Mus musculus | Ni2+ | — | 0.03218 | 1.605 | pH 8, 30 °C | Open record: ferrochelatase variant F323L, Ni2+ |
| ferrochelatasevariant F323L | Mus musculus | protoporphyrin IXaerobic; Ni2+ below 40 µM (no substrate inhibition) | 0.212 | 0.00123 | 173 | pH 8, 30 °C | Open record: ferrochelatase variant F323L, protoporphyrin IX |
| ferrochelatasevariant F323L | Mus musculus | protoporphyrin IXanaerobic; cosubstrate Fe2+ | 1.603 | 0.00547 | 292.8 | pH 8, 30 °C | Open record: ferrochelatase variant F323L, protoporphyrin IX |
| ferrochelatasevariant S143T | Mus musculus | Fe2+ | — | 0.00291 | 416.8 | pH 8, 30 °C | Open record: ferrochelatase variant S143T, Fe2+ |
| ferrochelatasevariant S143T | Mus musculus | Ni2+ | — | 0.06521 | 9.05 | pH 8, 30 °C | Open record: ferrochelatase variant S143T, Ni2+ |
| ferrochelatasevariant S143T | Mus musculus | protoporphyrin IXaerobic; Ni2+ below 40 µM (no substrate inhibition) | 0.59 | 2.9 × 10⁻⁴ | 2015 | pH 8, 30 °C | Open record: ferrochelatase variant S143T, protoporphyrin IX |
| ferrochelatasevariant S143T | Mus musculus | protoporphyrin IXanaerobic; cosubstrate Fe2+ | 1.212 | 0.00831 | 145.7 | pH 8, 30 °C | Open record: ferrochelatase variant S143T, protoporphyrin IX |
| ferrochelatasevariant S143T/F323L | Mus musculus | Fe2+ | — | 0.00728 | 261.3 | pH 8, 30 °C | Open record: ferrochelatase variant S143T/F323L, Fe2+ |
| ferrochelatasevariant S143T/F323L | Mus musculus | Ni2+ | — | 0.1037 | 4.23 | pH 8, 30 °C | Open record: ferrochelatase variant S143T/F323L, Ni2+ |
| ferrochelatasevariant S143T/F323L | Mus musculus | protoporphyrin IXaerobic; Ni2+ below 40 µM (no substrate inhibition) | 0.438 | 0.00243 | 180 | pH 8, 30 °C | Open record: ferrochelatase variant S143T/F323L, protoporphyrin IX |
| ferrochelatasevariant S143T/F323L | Mus musculus | protoporphyrin IXanaerobic; cosubstrate Fe2+ | 1.903 | 0.00558 | 340.5 | pH 8, 30 °C | Open record: ferrochelatase variant S143T/F323L, protoporphyrin IX |