EC 5.3.1.16: 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase
IsomerasesOpen in Browse
CatLog read 14 records for EC 5.3.1.16 from 3 papers, for 3 organisms. Names in these papers: ProFAR isomerase (HisA), 5′-ProFAR isomerase (HISN3), HisA.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 4 records for EC 5.3.1.16 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 5.3.1.16 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Two (betaalpha)(8)-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates | Escherichia coli, Thermotoga maritima | 10 |
| A sugar isomerization reaction established on various (βα)₈-barrel scaffolds is based on substrate-assisted catalysis | Thermotoga maritima | 2 |
| Structural, kinetic, and evolutionary peculiarities of HISN3, a plant 5'-ProFAR isomerase | Medicago truncatula | 2 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| 5′-ProFAR isomerase (HISN3) | Medicago truncatula | N’-[(5′-phosphoribosyl)formimino]-5-amino-imidazole-4-carboxamide ribonucleotide | 9.7 | 0.0046 | 2.1 × 10³ | pH 8, 22 °C | PMID 39186852 | Open record: 5′-ProFAR isomerase (HISN3), N’-[(5′-phosphoribosyl)formimino]-5-amino-imidazole-4-carboxamide ribonucleotide |
| 5′-ProFAR isomerase (HISN3)variant Δ74-80 | Medicago truncatula | N’-[(5′-phosphoribosyl)formimino]-5-amino-imidazole-4-carboxamide ribonucleotide | 0.8 | 0.0119 | 70 | pH 8, 22 °C | PMID 39186852 | Open record: 5′-ProFAR isomerase (HISN3) variant Δ74-80, N’-[(5′-phosphoribosyl)formimino]-5-amino-imidazole-4-carboxamide ribonucleotide |
| HisAvariant D127V/D169V | Thermotoga maritima | phosphoribosylanthranilate | 0.522 | 0.036 | 15 | pH 7.5, 25 °C | PMID 23109729 | Open record: HisA variant D127V/D169V, phosphoribosylanthranilate |
| HisAvariant D169V | Thermotoga maritima | phosphoribosylanthranilate | 0.028 | 0.036 | 0.78 | pH 7.5, 25 °C | PMID 23109729 | Open record: HisA variant D169V, phosphoribosylanthranilate |
| ProFAR isomerase (HisA) | Escherichia coli | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 4.9 | 0.0016 | 3.1 × 10³ | pH 7.5, 25 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA), N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |
| ProFAR isomerase (HisA) | Escherichia coli | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 14.3 | 0.0018 | 7.9 × 10³ | pH 7.5, 37 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA), N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |
| ProFAR isomerase (HisA) | Thermotoga maritima | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 0.67 | 6 × 10⁻⁴ | 1.1 × 10³ | pH 7.5, 25 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA), N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |
| ProFAR isomerase (HisA) | Thermotoga maritima | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 1.54 | 6 × 10⁻⁴ | 2.6 × 10³ | pH 7.5, 37 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA), N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |
| ProFAR isomerase (HisA) | Thermotoga maritima | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 32 | — | — | pH 7.5, 80 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA), N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |
| ProFAR isomerase (HisA)variant D127N | Thermotoga maritima | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 2.7 × 10⁻⁴ | 0.002 | 0.13 | pH 7.5, 25 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA) variant D127N, N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |
| ProFAR isomerase (HisA)variant D51N | Thermotoga maritima | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 0.29 | 0.0064 | 46 | pH 7.5, 25 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA) variant D51N, N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |
| ProFAR isomerase (HisA)variant H48A | Thermotoga maritima | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 0.38 | 0.0029 | 131 | pH 7.5, 25 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA) variant H48A, N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |
| ProFAR isomerase (HisA)variant R83N | Thermotoga maritima | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 0.23 | 0.0379 | 6 | pH 7.5, 25 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA) variant R83N, N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |
| ProFAR isomerase (HisA)variant T164A | Thermotoga maritima | N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide | 0.018 | 0.0056 | 3 | pH 7.5, 25 °C | PMID 12356303 | Open record: ProFAR isomerase (HisA) variant T164A, N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide |