CatLogenzyme kinetics catalog

EC 5.4.3.5: D-ornithine 4,5-aminomutase

IsomerasesOpen in Browse

CatLog read 23 records for EC 5.4.3.5 from 4 papers, for 1 organism. Names in these papers: ornithine 4,5-aminomutase (OAM), D-ornithine aminomutase (OraSE).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 14 records for EC 5.4.3.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 5.4.3.5 in Browse.

Papers

PaperOrganismsRecords
A conformational sampling model for radical catalysis in pyridoxal phosphate- and cobalamin-dependent enzymesClostridium sticklandii9
Optimal electrostatic interactions between substrate and protein are essential for radical chemistry in ornithine 4,5-aminomutaseClostridium sticklandii8
Cloning, sequencing, heterologous expression, purification, and characterization of adenosylcobalamin-dependent D-ornithine aminomutase from Clostridium sticklandiiClostridium sticklandii3
Mutagenesis of a conserved glutamate reveals the contribution of electrostatic energy to adenosylcobalamin co-C bond homolysis in ornithine 4,5-aminomutase and methylmalonyl-CoA mutaseClostridium sticklandii3

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
D-ornithine aminomutase (OraSE)Clostridium sticklandii5'-deoxyadenosylcobalamin—4.3 × 10⁻⁴—pH 8.5PMID 11577113Open
D-ornithine aminomutase (OraSE)Clostridium sticklandiiD-ornithine6.30.0445140 calc.pH 8.5PMID 11577113Open
D-ornithine aminomutase (OraSE)Clostridium sticklandiipyridoxal 5'-phosphate—0.0015—pH 8.5PMID 11577113Open
ornithine 4,5-aminomutase (OAM)Clostridium sticklandiiD-ornithine2.970.18915.7pH 8.5, 25 °CPMID 25213862Open
ornithine 4,5-aminomutase (OAM)Clostridium sticklandiiD-ornithineMTSL nitroxide spin-labeled at Cys352 and Cys7002.860.18615.4pH 8.5, 25 °CPMID 25213862Open
ornithine 4,5-aminomutase (OAM)Clostridium sticklandiiD-ornithine8.20.043190PMID 28528213Open
ornithine 4,5-aminomutase (OAM)variant C700SClostridium sticklandiiD-ornithine2.880.18615.5pH 8.5, 25 °CPMID 25213862Open
ornithine 4,5-aminomutase (OAM)variant C700SClostridium sticklandiiD-ornithineMTSL nitroxide spin-labeled at Cys3522.780.18914.7pH 8.5, 25 °CPMID 25213862Open
ornithine 4,5-aminomutase (OAM)variant D627AClostridium sticklandiiD-ornithine2.890.19315pH 8.5, 25 °CPMID 25213862Open
ornithine 4,5-aminomutase (OAM)variant E338AClostridium sticklandiiD-ornithine0.00430.06060.07PMID 23311430Open
ornithine 4,5-aminomutase (OAM)variant E338AClostridium sticklandiiD-ornithine0.240.1761.34pH 8.5, 25 °CPMID 25213862Open
ornithine 4,5-aminomutase (OAM)variant E338DClostridium sticklandiiD-ornithine0.00760.03020.25PMID 23311430Open
ornithine 4,5-aminomutase (OAM)variant E338QClostridium sticklandiiD-ornithine0.0320.04270.75PMID 23311430Open
ornithine 4,5-aminomutase (OAM)variant E81AClostridium sticklandiiD-ornithine0.0130.780.016PMID 28528213Open
ornithine 4,5-aminomutase (OAM)variant E81DClostridium sticklandiiD-ornithine0.002——PMID 28528213Open
ornithine 4,5-aminomutase (OAM)variant E81QClostridium sticklandiiD-ornithine0.0290.870.33PMID 28528213Open
ornithine 4,5-aminomutase (OAM)variant G339WClostridium sticklandiiD-ornithine0.20.191.05pH 8.5, 25 °CPMID 25213862Open
ornithine 4,5-aminomutase (OAM)variant I424EClostridium sticklandiiD-ornithine0.760.1854.12pH 8.5, 25 °CPMID 25213862Open
ornithine 4,5-aminomutase (OAM)variant N226DClostridium sticklandiiD-ornithine0.380.123.2PMID 28528213Open
ornithine 4,5-aminomutase (OAM)variant P343WClostridium sticklandiiD-ornithine0.140.1930.73pH 8.5, 25 °CPMID 25213862Open
ornithine 4,5-aminomutase (OAM)variant R297KClostridium sticklandiiD-ornithine0.0684.90.014PMID 28528213Open
ornithine 4,5-aminomutase (OAM)variant S162AClostridium sticklandiiD-ornithine0.370.02913PMID 28528213Open
ornithine 4,5-aminomutase (OAM)variant Y160FClostridium sticklandiiD-ornithine0.0270.140.19PMID 28528213Open