EC 6.1.1.15: proline-tRNA ligase
LigasesOpen in Browse
CatLog read 38 records for EC 6.1.1.15 from 3 papers, for 13 organisms. Names in these papers: prolyl-tRNA synthetase (ProRS).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 81 records for EC 6.1.1.15 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.1.1.15 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Cysteine activation is an inherent in vitro property of prolyl-tRNA synthetases | Aquifex aeolicus, Borrelia burgdorferi and 11 more | 26 |
| Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation | Methanothermobacter thermautotrophicus | 6 |
| Role of coupled dynamics in the catalytic activity of prokaryotic-like prolyl-tRNA synthetases | Escherichia coli | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| prolyl-tRNA synthetase (ProRS) | Aquifex aeolicus | L-cysteine | 0.09 | 0.05 | 1.8 calc. | pH 7.2, 60 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Aquifex aeolicus | L-proline | 15 | 0.06 | 250 calc. | pH 7.2, 60 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Borrelia burgdorferi | L-cysteine | 0.022 | 0.18 | 0.12 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Borrelia burgdorferi | L-proline | 5.8 | 0.17 | 34 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Clostridium sticklandii | L-cysteine | 0.39 | 0.01 | 39 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Clostridium sticklandii | L-proline | 60 | 0.05 | 1.2 × 10³ calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Cytophaga hutchinsonii | L-cysteine | 0.083 | 0.19 | 0.44 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Cytophaga hutchinsonii | L-proline | 19 | 0.08 | 240 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Deinococcus radiodurans | L-cysteine | 0.014 | 0.01 | 1.4 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Deinococcus radiodurans | L-proline | 32 | 0.16 | 200 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Escherichia coli | alanine | 3.52 | 685 | 0.00513 | 37 °C | PMID 22356126 | Open record: prolyl-tRNA synthetase (ProRS), alanine |
| prolyl-tRNA synthetase (ProRS) | Escherichia coli | L-cysteine | 0.022 | 0.17 | 0.13 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Escherichia coli | L-proline | 14 | 0.29 | 48 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Escherichia coli | proline | 12.7 | 0.228 | 55.7 | 37 °C | PMID 22356126 | Open record: prolyl-tRNA synthetase (ProRS), proline |
| prolyl-tRNA synthetase (ProRS)variant E218A | Escherichia coli | alanine | 3.26 | 1.36 × 10³ | 0.0024 | 37 °C | PMID 22356126 | Open record: prolyl-tRNA synthetase (ProRS) variant E218A, alanine |
| prolyl-tRNA synthetase (ProRS)variant E218A | Escherichia coli | proline | 4.4 | 3.4 | 1.29 | 37 °C | PMID 22356126 | Open record: prolyl-tRNA synthetase (ProRS) variant E218A, proline |
| prolyl-tRNA synthetase (ProRS)variant G217A | Escherichia coli | alanine | 2.18 | 454 | 0.0048 | 37 °C | PMID 22356126 | Open record: prolyl-tRNA synthetase (ProRS) variant G217A, alanine |
| prolyl-tRNA synthetase (ProRS)variant G217A | Escherichia coli | proline | 3.37 | 0.427 | 7.89 | 37 °C | PMID 22356126 | Open record: prolyl-tRNA synthetase (ProRS) variant G217A, proline |
| prolyl-tRNA synthetase (ProRS) | Magnetospirillum magnetotacticum | L-cysteine | 0.11 | 0.26 | 0.42 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Magnetospirillum magnetotacticum | L-proline | 35 | 0.14 | 250 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Methanocaldococcus jannaschii | L-cysteine | 0.19 | 0.09 | 2.1 calc. | pH 7.2, 60 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Methanocaldococcus jannaschii | L-proline | 63 | 0.28 | 220 calc. | pH 7.2, 60 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Methanothermobacter thermautotrophicus | L-cysteine | 0.02 | 0.05 | 0.40 calc. | pH 7.2, 60 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Methanothermobacter thermautotrophicus | L-proline | 3.4 | 0.26 | 13 calc. | pH 7.2, 60 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Methanothermobacter thermautotrophicus | L-prolineBSA added | — | 0.067 | — | pH 7.5, 50 °C | PMID 15917221 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Methanothermobacter thermautotrophicus | L-prolineLeuRS and 400 nM BSA added (ProRS-LeuRS complex) | — | 0.061 | — | pH 7.5, 50 °C | PMID 15917221 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Methanothermobacter thermautotrophicus | tRNAProno addition | 1.8 | 0.0041 | 440 calc. | pH 7.5, 50 °C | PMID 15917221 | Open record: prolyl-tRNA synthetase (ProRS), tRNAPro |
| prolyl-tRNA synthetase (ProRS) | Methanothermobacter thermautotrophicus | tRNAProwith 400 nM LeuRS added (ProRS-LeuRS complex) | 5.1 | 0.0022 | 2.3 × 10³ calc. | pH 7.5, 50 °C | PMID 15917221 | Open record: prolyl-tRNA synthetase (ProRS), tRNAPro |
| prolyl-tRNA synthetase (ProRS) | Methanothermobacter thermautotrophicus | tRNAProwith 400 nM BSA added | 2.9 | 0.0024 | 1.2 × 10³ calc. | pH 7.5, 50 °C | PMID 15917221 | Open record: prolyl-tRNA synthetase (ProRS), tRNAPro |
| prolyl-tRNA synthetase (ProRS) | Methanothermobacter thermautotrophicus | tRNAProwith 400 nM LeuRS and 400 nM BSA added (ProRS-LeuRS complex) | 6.9 | 0.0014 | 4.9 × 10³ calc. | pH 7.5, 50 °C | PMID 15917221 | Open record: prolyl-tRNA synthetase (ProRS), tRNAPro |
| prolyl-tRNA synthetase (ProRS) | Novosphingobium aromaticivorans | L-cysteine | 0.065 | 0.2 | 0.33 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Novosphingobium aromaticivorans | L-proline | 26 | 0.14 | 190 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Rhodopseudomonas palustris | L-cysteine | 0.025 | 0.17 | 0.15 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Rhodopseudomonas palustris | L-proline | 14 | 0.28 | 50 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Saccharomyces cerevisiae | L-cysteine | 0.003 | 0.03 | 0.10 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Saccharomyces cerevisiae | L-proline | 5.6 | 0.11 | 51 calc. | pH 7.2, 37 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |
| prolyl-tRNA synthetase (ProRS) | Thermus thermophilus | L-cysteine | 0.042 | 0.02 | 2.1 calc. | pH 7.2, 60 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-cysteine |
| prolyl-tRNA synthetase (ProRS) | Thermus thermophilus | L-proline | 35 | 0.15 | 230 calc. | pH 7.2, 60 °C | PMID 12130657 | Open record: prolyl-tRNA synthetase (ProRS), L-proline |