Values CatLog read from this paper

Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation

PMID 15917221doi:10.1074/jbc.m503539200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 10 records (14 values) from this paper: prolyl-tRNA synthetase (ProRS), leucyl-tRNA synthetase (LeuRS) from Methanothermobacter thermautotrophicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.1.1.4, EC 6.1.1.15

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
leucyl-tRNA synthetase (LeuRS)Methanothermobacter thermautotrophicusL-leucineBSA added——350pH 7.5, 50 °COpen record: leucyl-tRNA synthetase (LeuRS), L-leucine
leucyl-tRNA synthetase (LeuRS)Methanothermobacter thermautotrophicusL-leucineProRS and 400 nM BSA added (LeuRS-ProRS complex)——370pH 7.5, 50 °COpen record: leucyl-tRNA synthetase (LeuRS), L-leucine
leucyl-tRNA synthetase (LeuRS)Methanothermobacter thermautotrophicustRNALeuBSA added——750pH 7.5, 50 °COpen record: leucyl-tRNA synthetase (LeuRS), tRNALeu
leucyl-tRNA synthetase (LeuRS)Methanothermobacter thermautotrophicustRNALeuProRS and 400 nM BSA added (LeuRS-ProRS complex)——750pH 7.5, 50 °COpen record: leucyl-tRNA synthetase (LeuRS), tRNALeu
prolyl-tRNA synthetase (ProRS)Methanothermobacter thermautotrophicusL-prolineBSA added—0.067—pH 7.5, 50 °COpen record: prolyl-tRNA synthetase (ProRS), L-proline
prolyl-tRNA synthetase (ProRS)Methanothermobacter thermautotrophicusL-prolineLeuRS and 400 nM BSA added (ProRS-LeuRS complex)—0.061—pH 7.5, 50 °COpen record: prolyl-tRNA synthetase (ProRS), L-proline
prolyl-tRNA synthetase (ProRS)Methanothermobacter thermautotrophicustRNAProno addition1.80.0041440 calc.pH 7.5, 50 °COpen record: prolyl-tRNA synthetase (ProRS), tRNAPro
prolyl-tRNA synthetase (ProRS)Methanothermobacter thermautotrophicustRNAProwith 400 nM LeuRS added (ProRS-LeuRS complex)5.10.00222.3 × 10³ calc.pH 7.5, 50 °COpen record: prolyl-tRNA synthetase (ProRS), tRNAPro
prolyl-tRNA synthetase (ProRS)Methanothermobacter thermautotrophicustRNAProwith 400 nM BSA added2.90.00241.2 × 10³ calc.pH 7.5, 50 °COpen record: prolyl-tRNA synthetase (ProRS), tRNAPro
prolyl-tRNA synthetase (ProRS)Methanothermobacter thermautotrophicustRNAProwith 400 nM LeuRS and 400 nM BSA added (ProRS-LeuRS complex)6.90.00144.9 × 10³ calc.pH 7.5, 50 °COpen record: prolyl-tRNA synthetase (ProRS), tRNAPro