EC 6.1.1.4: leucine-tRNA ligase

LigasesOpen in Browse

CatLog read 308 records for EC 6.1.1.4 from 15 papers, for 9 organisms. Names in these papers: leucyl-tRNA synthetase (PhLeuRS), leucyl-tRNA synthetase (GlLeuRS), αβ-LeuRS (leucyl-tRNA synthetase), leucyl-tRNA synthetase (EcLeuRS) and 15 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 117 records for EC 6.1.1.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.1.1.4 in Browse.

Papers

Papers with records for EC 6.1.1.4
Paper	Organisms	Records
Functional characterization of leucine-specific domain 1 from eukaryal and archaeal leucyl-tRNA synthetases	Giardia lamblia, Pyrococcus horikoshii	102
Modulation of Aminoacylation and Editing Properties of Leucyl-tRNA Synthetase by a Conserved Structural Module	Aquifex aeolicus, Escherichia coli and 3 more	41
A single residue in leucyl-tRNA synthetase affecting amino acid specificity and tRNA aminoacylation	Escherichia coli, Homo sapiens	31
Two distinct domains of the beta subunit of Aquifex aeolicus leucyl-tRNA synthetase are involved in tRNA binding as revealed by a three-hybrid selection	Aquifex aeolicus	26
Coexistence of bacterial leucyl-tRNA synthetases with archaeal tRNA binding domains that distinguish tRNA(Leu) in the archaeal mode	Natrialba magadii	19
Leucyl-tRNA synthetase consisting of two subunits from hyperthermophilic bacteria Aquifex aeolicus	Aquifex aeolicus	13
A unique insertion in the CP1 domain of Giardia lamblia leucyl-tRNA synthetase	Giardia lamblia	13
Crucial role of the C-terminal domain of Mycobacterium tuberculosis leucyl-tRNA synthetase in aminoacylation and editing	Mycobacterium tuberculosis	13
The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex	Homo sapiens	12
Two tyrosine residues outside the editing active site in Giardia lamblia leucyl-tRNA synthetase are essential for the post-transfer editing	Giardia lamblia	10
Groups on the side chain of T252 in Escherichia coli leucyl-tRNA synthetase are important for discrimination of amino acids and cell viability	Escherichia coli	9
The processing of human mitochondrial leucyl-tRNA synthetase in the insect cells	Homo sapiens	6
A paradigm shift for the amino acid editing mechanism of human cytoplasmic leucyl-tRNA synthetase	Homo sapiens	6
Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation	Methanothermobacter thermautotrophicus	4
The ZN domain acts as a dynamic switch coordinating multiple-step aminoacylation in bacterial leucyl-tRNA synthetase	Escherichia coli	3

Records

Records for EC 6.1.1.4 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
cytosolic leucyl-tRNA synthetase (hcLeuRS)	Homo sapiens	ATP8 mM KF; 4 mM leucine	0.79	1.308	0.6	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS), ATP
cytosolic leucyl-tRNA synthetase (hcLeuRS)	Homo sapiens	ATP100 µM [14C]leucine (70 cpm/pmol)	0.27	0.773	0.35	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS), ATP
cytosolic leucyl-tRNA synthetase (hcLeuRS)	Homo sapiens	calf liver tRNA-Leu	0.28	0.0019	147	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS), calf liver tRNA-Leu
cytosolic leucyl-tRNA synthetase (hcLeuRS)	Homo sapiens	human cytosolic tRNA-Leu(AAG) in vitro transcript (unmodified)	0.25	0.001	250	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS), human cytosolic tRNA-Leu(AAG) in vitro transcript (unmodified)
cytosolic leucyl-tRNA synthetase (hcLeuRS)	Homo sapiens	leucine8 mM KF; 4 mM leucine	0.82	0.075	11	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS), leucine
cytosolic leucyl-tRNA synthetase (hcLeuRS)	Homo sapiens	leucine100 µM [14C]leucine (70 cpm/pmol)	0.3	0.0075	40	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS), leucine
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRS	Homo sapiens	ATP8 mM KF; 4 mM leucine	0.74	1.349	0.55	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS) variant ΔChcLeuRS, ATP
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRS	Homo sapiens	ATP100 µM [14C]leucine (70 cpm/pmol)	0.55	0.725	0.76	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS) variant ΔChcLeuRS, ATP
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRS	Homo sapiens	calf liver tRNA-Leu	0.59	0.0014	421	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS) variant ΔChcLeuRS, calf liver tRNA-Leu
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRS	Homo sapiens	human cytosolic tRNA-Leu(AAG) in vitro transcript (unmodified)	0.5	9.4 × 10⁻⁴	532	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS) variant ΔChcLeuRS, human cytosolic tRNA-Leu(AAG) in vitro transcript (unmodified)
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRS	Homo sapiens	leucine8 mM KF; 4 mM leucine	0.81	0.064	13	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS) variant ΔChcLeuRS, leucine
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRS	Homo sapiens	leucine100 µM [14C]leucine (70 cpm/pmol)	0.56	0.0058	97	pH 7.6, 37 °C	PMID 16055448	Open record: cytosolic leucyl-tRNA synthetase (hcLeuRS) variant ΔChcLeuRS, leucine
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)	Escherichia coli	Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)	14.5	4 × 10⁻⁴	3.6 × 10⁴	pH 7, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS), Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)	Escherichia coli	human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)	0.0016	0.025	0.0064	pH 7, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS), human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)	Escherichia coli	isoleucine	0.51	0.14	3.6	pH 7.5, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS), isoleucine
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)	Escherichia coli	leucine	29	0.013	2.2 × 10³	pH 7.5, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS), leucine
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570F	Escherichia coli	Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)	24	0.0029	8 × 10³	pH 7, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570F, Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570F	Escherichia coli	human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)	0.0029	0.0017	1.7	pH 7, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570F, human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570F	Escherichia coli	isoleucine	0.55	0.05	10.8	pH 7.5, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570F, isoleucine
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570F	Escherichia coli	leucine	35	0.15	230	pH 7.5, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570F, leucine
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570K	Escherichia coli	Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)	9.8	9 × 10⁻⁴	1.1 × 10⁴	pH 7, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570K, Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570K	Escherichia coli	human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)	1.8 × 10⁻⁵	0.002	0.009	pH 7, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570K, human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570K	Escherichia coli	isoleucine	0.76	1.5	0.5	pH 7.5, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570K, isoleucine
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570K	Escherichia coli	leucine	37	0.013	2.8 × 10³	pH 7.5, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570K, leucine
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570R	Escherichia coli	Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)	21	0.0015	1.4 × 10⁴	pH 7, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570R, Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570R	Escherichia coli	isoleucine	0.23	0.09	2.6	pH 7.5, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570R, isoleucine
Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS)variant L570R	Escherichia coli	leucine	43	0.003	1.43 × 10⁴	pH 7.5, 37 °C	PMID 17378584	Open record: Escherichia coli leucyl-tRNA synthetase (E. coli LeuRS) variant L570R, leucine
human cytoplasmic leucyl-tRNA synthetase (hscLeuRS)	Homo sapiens	isoleucine	0.34	2.04	0.16	pH 8	PMID 19702327	Open record: human cytoplasmic leucyl-tRNA synthetase (hscLeuRS), isoleucine
human cytoplasmic leucyl-tRNA synthetase (hscLeuRS)	Homo sapiens	leucine	2.1	0.024	94.3	pH 8	PMID 19702327	Open record: human cytoplasmic leucyl-tRNA synthetase (hscLeuRS), leucine
human cytoplasmic leucyl-tRNA synthetase (hscLeuRS)variant D399A	Homo sapiens	isoleucine	0.51	3.3	0.17	pH 8	PMID 19702327	Open record: human cytoplasmic leucyl-tRNA synthetase (hscLeuRS) variant D399A, isoleucine
human cytoplasmic leucyl-tRNA synthetase (hscLeuRS)variant D399A	Homo sapiens	isoleucine	0.0018	—	—	pH 7.6, 30 °C	PMID 19702327	Open record: human cytoplasmic leucyl-tRNA synthetase (hscLeuRS) variant D399A, isoleucine
human cytoplasmic leucyl-tRNA synthetase (hscLeuRS)variant D399A	Homo sapiens	isoleucine	0.06	1.8	0.033 calc.	pH 7.5	PMID 19702327	Open record: human cytoplasmic leucyl-tRNA synthetase (hscLeuRS) variant D399A, isoleucine
human cytoplasmic leucyl-tRNA synthetase (hscLeuRS)variant D399A	Homo sapiens	leucine	5.6	0.9	5.6	pH 8	PMID 19702327	Open record: human cytoplasmic leucyl-tRNA synthetase (hscLeuRS) variant D399A, leucine
human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B)	Homo sapiens	adenosine 5'-triphosphateATP-PPi exchange reaction; L-leucine	0.13	0.127	1	37 °C	PMID 12527375	Open record: human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B), adenosine 5'-triphosphate
human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B)	Homo sapiens	adenosine 5'-triphosphateleucylation of tRNA^Leu reaction	0.049	1.192	0.041	37 °C	PMID 12527375	Open record: human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B), adenosine 5'-triphosphate
human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B)	Homo sapiens	Aquifex aeolicus tRNA^Leu(GAG)	0.019	0.0028	6.8	37 °C	PMID 12527375	Open record: human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B), Aquifex aeolicus tRNA^Leu(GAG)
human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B)	Homo sapiens	Escherichia coli tRNA1^Leu(CAG)	0.041	0.0022	18.5	37 °C	PMID 12527375	Open record: human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B), Escherichia coli tRNA1^Leu(CAG)
human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B)	Homo sapiens	L-leucineATP-PPi exchange reaction; ATP	0.14	0.25	0.56	37 °C	PMID 12527375	Open record: human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B), L-leucine
human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B)	Homo sapiens	L-leucineleucylation of tRNA^Leu reaction	0.053	0.0397	1.33	37 °C	PMID 12527375	Open record: human mitochondrial leucyl-tRNA synthetase (hmLeuRS-B), L-leucine
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)	Homo sapiens	Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)	0.39	2 × 10⁻⁴	2 × 10³	pH 7, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS), Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)	Homo sapiens	human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)	0.09	0.004	24	pH 7, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS), human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)	Homo sapiens	isoleucine	0.44	11	0.04	pH 7.5, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS), isoleucine
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)	Homo sapiens	leucine	7.9	0.15	50	pH 7.5, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS), leucine
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600F	Homo sapiens	Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)	3	0.0022	1.4 × 10³	pH 7, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600F, Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600F	Homo sapiens	human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)	0.13	0.006	20	pH 7, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600F, human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600F	Homo sapiens	isoleucine	0.5	0.9	0.58	pH 7.5, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600F, isoleucine
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600F	Homo sapiens	leucine	25	0.33	80	pH 7.5, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600F, leucine
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600L	Homo sapiens	Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)	0.35	0.0018	190	pH 7, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600L, Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600L	Homo sapiens	human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)	0.0027	0.0015	1.8	pH 7, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600L, human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600L	Homo sapiens	isoleucine	0.14	11	0.013	pH 7.5, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600L, isoleucine
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600L	Homo sapiens	leucine	5.9	0.03	200	pH 7.5, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600L, leucine
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600R	Homo sapiens	Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)	3.2	0.0013	1.8 × 10³	pH 7, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600R, Escherichia coli tRNALeu(CUN) (T7 in vitro transcript)
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600R	Homo sapiens	human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)	0.14	0.004	30	pH 7, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600R, human mitochondrial tRNALeu(UUR) (T7 in vitro transcript)
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600R	Homo sapiens	isoleucine	0.52	1.1	0.49	pH 7.5, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600R, isoleucine
human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS)variant K600R	Homo sapiens	leucine	27	0.07	420	pH 7.5, 37 °C	PMID 17378584	Open record: human mitochondrial leucyl-tRNA synthetase (hs mt LeuRS) variant K600R, leucine
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	1.5	3 × 10⁻⁴	5 × 10³	65 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer), Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R94A	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	1.3	7.5 × 10⁻⁴	1.7 × 10³	65 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R94A, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R94A/R98A	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	0.67	8.1 × 10⁻⁴	830	65 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R94A/R98A, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R94E	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	0.89	8.1 × 10⁻⁴	1.1 × 10³	65 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R94E, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R94E/R98E	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	0.19	8.8 × 10⁻⁴	220	65 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R94E/R98E, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R98A	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	1.5	3.1 × 10⁻⁴	4.8 × 10³	65 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R98A, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R98E	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	1	3.9 × 10⁻⁴	2.6 × 10³	65 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R98E, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	4.2	0.0016	2.6 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C40U mutant	3.5	0.0015	1.9 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C40U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C41U mutant	1.9	0.0014	1.3 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C41U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C41U/C42U mutant	1.3	0.0042	310	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C41U/C42U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C42U mutant	1.8	0.0027	670	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C42U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), G12U mutant	3.9	0.0017	2.3 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), G12U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), G13U mutant	4	0.0017	2.4 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), G13U mutant
leucyl-tRNA synthetase (EcLeuRS)variant D653K	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	3.1	0.0022	1.4 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant D653K, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G662A/G665A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.7	0.0012	580	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G662A/G665A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G662P	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	2.2	0.0011	2 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G662P, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G662P/G665P	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.3	0.0014	210	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G662P/G665P, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G662R/G665R	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.8	0.0013	610	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G662R/G665R, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G665P	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	2.1	0.0013	1.6 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G665P, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant K671A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	4.8	0.0024	2 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant K671A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant L656K/E657K	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	1.1	6.6 × 10⁻⁴	1.7 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant L656K/E657K, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R668A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	1.4	0.0028	500	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R668A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R668A/R672A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.31	0.0022	140	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R668A/R672A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R668E	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.59	0.0054	110	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R668E, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R668E/R672E	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.18	0.0058	30	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R668E/R672E, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R672A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	4.8	0.0024	2 × 10³	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R672A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R672E	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.53	0.0028	190	pH 7.8, 37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R672E, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	adenosine 5'-triphosphate1 mM Leu; 2 mM tetrasodium [32P]pyrophosphate; 20 nM GlLeuRS	34.5	0.7504	46	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS), adenosine 5'-triphosphate
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	adenosine 5'-triphosphate10 µM tRNALeu; 20 µM [3H]Leu; cosubstrate L-leucine, tRNALeu	3.1	0.6876	4.5	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS), adenosine 5'-triphosphate
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	Aquifex aeolicus tRNALeu(GAG), purified from an overproducing strain	0.8	0.0075	106.7	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS), Aquifex aeolicus tRNALeu(GAG), purified from an overproducing strain
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	Escherichia coli tRNALeu(GAG), purified from an overproducing strain	2.6	0.0072	361.1	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS), Escherichia coli tRNALeu(GAG), purified from an overproducing strain
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)	2.7	0.0061	442.6	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS), Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	isoleucyl-tRNALeu (mischarged G. lamblia tRNALeu(AAG))	1.52	0.0092	165	pH 7.5, 37 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS), isoleucyl-tRNALeu (mischarged G. lamblia tRNALeu(AAG))
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	L-leucine1 mM Leu; 2 mM tetrasodium [32P]pyrophosphate; 20 nM GlLeuRS	30.5	0.0142	2148	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS), L-leucine
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	L-leucine10 µM tRNALeu; 20 µM [3H]Leu; cosubstrate ATP, tRNALeu	2.8	0.009	311.1	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS), L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant D173A	Giardia lamblia	ATP	34.4	1.025	33.5	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant D173A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant D173A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	0.8	0.0059	136		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant D173A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant D173A	Giardia lamblia	L-leucine	36.3	0.0165	2.2 × 10³	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant D173A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant E165A	Giardia lamblia	ATP	31.3	1.169	26.8	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant E165A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant E165A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	2.8	0.0083	337		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant E165A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant E165A	Giardia lamblia	L-leucine	29.7	0.0157	1892	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant E165A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant E167A	Giardia lamblia	ATP	60.2	2.129	28.3	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant E167A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant E167A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	1.2	0.0049	245		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant E167A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant E167A	Giardia lamblia	L-leucine	54.6	0.0162	3.37 × 10³	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant E167A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant K139A	Giardia lamblia	ATP	33.2	0.812	40.9	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K139A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant K139A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	1.5	0.0114	132		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K139A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant K139A	Giardia lamblia	L-leucine	29.9	0.0151	1.98 × 10³	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K139A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant K141A	Giardia lamblia	ATP	31.5	0.772	40.8	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K141A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant K141A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	0.8	0.0051	157		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K141A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant K141A	Giardia lamblia	L-leucine	33.1	0.0132	2508	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K141A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant K142A	Giardia lamblia	ATP	36.2	0.728	49.7	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K142A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant K142A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	0.2	0.0035	57		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K142A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant K142A	Giardia lamblia	L-leucine	31.7	0.0145	2186	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K142A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant K144A	Giardia lamblia	ATP	31.8	0.822	38.7	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K144A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant K144A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	0.2	0.0052	38		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K144A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant K144A	Giardia lamblia	L-leucine	28.9	0.0153	1889	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K144A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant K148A	Giardia lamblia	ATP	32.1	0.698	46	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K148A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant K148A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	1.9	0.005	380		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K148A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant K148A	Giardia lamblia	L-leucine	28.4	0.0124	2.29 × 10³	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K148A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant K152A	Giardia lamblia	ATP	8.1	0.834	9.7	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K152A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant K152A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	1.3	0.0051	255		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K152A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant K152A	Giardia lamblia	L-leucine	6.2	0.0024	2583	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K152A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant K166A	Giardia lamblia	ATP	32.3	0.687	47	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K166A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant K166A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	2.8	0.0081	346		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K166A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant K166A	Giardia lamblia	L-leucine	28.7	0.0147	1952	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K166A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant K170A	Giardia lamblia	ATP	31	0.683	45.4	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K170A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant K170A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	1.4	0.0051	275		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K170A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant K170A	Giardia lamblia	L-leucine	28.1	0.0141	1993	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant K170A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant Q154A	Giardia lamblia	ATP	7.5	0.675	11.1	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Q154A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant Q154A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	0.8	0.0038	210		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Q154A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant Q154A	Giardia lamblia	L-leucine	5.9	0.0035	1686	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Q154A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant S153A	Giardia lamblia	ATP	30.1	0.711	42.3	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant S153A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant S153A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	1.1	0.004	275		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant S153A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant S153A	Giardia lamblia	L-leucine	32.5	0.0159	2044	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant S153A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant W155A	Giardia lamblia	ATP	29.8	0.653	45.6	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant W155A, ATP
leucyl-tRNA synthetase (GlLeuRS)variant W155A	Giardia lamblia	Giardia lamblia tRNALeu (AAG), in vitro T7 transcript	2.5	0.0071	352		PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant W155A, Giardia lamblia tRNALeu (AAG), in vitro T7 transcript
leucyl-tRNA synthetase (GlLeuRS)variant W155A	Giardia lamblia	L-leucine	31.9	0.0119	2681	pH 8.2, 45 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (GlLeuRS) variant W155A, L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant Y515A	Giardia lamblia	Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript	2.6	0.0054	481.5	pH 8.2, 45 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Y515A, Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript
leucyl-tRNA synthetase (GlLeuRS)variant Y515A	Giardia lamblia	isoleucyl-tRNALeu (mischarged G. lamblia tRNALeu(AAG))	0.19	0.0122	16	pH 7.5, 37 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Y515A, isoleucyl-tRNALeu (mischarged G. lamblia tRNALeu(AAG))
leucyl-tRNA synthetase (GlLeuRS)variant Y515E	Giardia lamblia	Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript	1	0.0054	185.2	pH 8.2, 45 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Y515E, Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript
leucyl-tRNA synthetase (GlLeuRS)variant Y515K	Giardia lamblia	Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript	1.8	0.008	225	pH 8.2, 45 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Y515K, Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript
leucyl-tRNA synthetase (GlLeuRS)variant Y520A	Giardia lamblia	Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript	1.8	0.006	300	pH 8.2, 45 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Y520A, Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript
leucyl-tRNA synthetase (GlLeuRS)variant Y520A	Giardia lamblia	isoleucyl-tRNALeu (mischarged G. lamblia tRNALeu(AAG))	0.48	0.0173	28	pH 7.5, 37 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Y520A, isoleucyl-tRNALeu (mischarged G. lamblia tRNALeu(AAG))
leucyl-tRNA synthetase (GlLeuRS)variant Y520E	Giardia lamblia	Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript	1.4	0.0077	181.8	pH 8.2, 45 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Y520E, Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript
leucyl-tRNA synthetase (GlLeuRS)variant Y520H	Giardia lamblia	Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript	1.1	0.0054	203.7	pH 8.2, 45 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Y520H, Giardia lamblia tRNALeu(AAG), T7 RNA polymerase transcript
leucyl-tRNA synthetase (GlLeuRS)variant Y520H	Giardia lamblia	isoleucyl-tRNALeu (mischarged G. lamblia tRNALeu(AAG))	0.93	0.0147	63	pH 7.5, 37 °C	PMID 19540202	Open record: leucyl-tRNA synthetase (GlLeuRS) variant Y520H, isoleucyl-tRNALeu (mischarged G. lamblia tRNALeu(AAG))
leucyl-tRNA synthetase (GlLeuRS)variant ΔQ281-D294 (GlLeuRS-A)	Giardia lamblia	adenosine 5'-triphosphate	1.2	0.33	3.6	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔQ281-D294 (GlLeuRS-A), adenosine 5'-triphosphate
leucyl-tRNA synthetase (GlLeuRS)variant ΔQ281-D294 (GlLeuRS-A)	Giardia lamblia	Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)	1.3	0.0101	128.7	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔQ281-D294 (GlLeuRS-A), Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)
leucyl-tRNA synthetase (GlLeuRS)variant ΔQ281-D294 (GlLeuRS-A)	Giardia lamblia	L-leucine	1.1	0.0059	186.4	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔQ281-D294 (GlLeuRS-A), L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant ΔS295-L304 (GlLeuRS-B)	Giardia lamblia	adenosine 5'-triphosphate	0.9	0.4153	2.2	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔS295-L304 (GlLeuRS-B), adenosine 5'-triphosphate
leucyl-tRNA synthetase (GlLeuRS)variant ΔS295-L304 (GlLeuRS-B)	Giardia lamblia	Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)	0.6	0.0082	73.2	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔS295-L304 (GlLeuRS-B), Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)
leucyl-tRNA synthetase (GlLeuRS)variant ΔS295-L304 (GlLeuRS-B)	Giardia lamblia	L-leucine	0.6	0.0077	77.9	pH 8.2, 45 °C	PMID 19170608	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔS295-L304 (GlLeuRS-B), L-leucine
leucyl-tRNA synthetase (LeuRS)	Escherichia coli	adenosine 5'-triphosphate	5	0.24	21	pH 7.8, 37 °C	PMID 15110746	Open record: leucyl-tRNA synthetase (LeuRS), adenosine 5'-triphosphate
leucyl-tRNA synthetase (LeuRS)	Escherichia coli	E. coli tRNA(Leu)	5.1	0.0026	1923	pH 7.8, 37 °C	PMID 15110746	Open record: leucyl-tRNA synthetase (LeuRS), E. coli tRNA(Leu)
leucyl-tRNA synthetase (LeuRS)	Escherichia coli	L-leucine	5.1	0.02	255	pH 7.8, 37 °C	PMID 15110746	Open record: leucyl-tRNA synthetase (LeuRS), L-leucine
leucyl-tRNA synthetase (LeuRS)	Escherichia coli	L-leucine	—	—	1.78 × 10³	pH 7.5, 37 °C	PMID 42573068	Open record: leucyl-tRNA synthetase (LeuRS), L-leucine
leucyl-tRNA synthetase (LeuRS)variant T252D	Escherichia coli	adenosine 5'-triphosphate	4.8	0.23	21	pH 7.8, 37 °C	PMID 15110746	Open record: leucyl-tRNA synthetase (LeuRS) variant T252D, adenosine 5'-triphosphate
leucyl-tRNA synthetase (LeuRS)variant T252D	Escherichia coli	E. coli tRNA(Leu)	4.7	0.0025	1.88 × 10³	pH 7.8, 37 °C	PMID 15110746	Open record: leucyl-tRNA synthetase (LeuRS) variant T252D, E. coli tRNA(Leu)
leucyl-tRNA synthetase (LeuRS)variant T252D	Escherichia coli	L-leucine	4.6	0.019	242	pH 7.8, 37 °C	PMID 15110746	Open record: leucyl-tRNA synthetase (LeuRS) variant T252D, L-leucine
leucyl-tRNA synthetase (LeuRS)variant T252E	Escherichia coli	adenosine 5'-triphosphate	4.9	0.22	22	pH 7.8, 37 °C	PMID 15110746	Open record: leucyl-tRNA synthetase (LeuRS) variant T252E, adenosine 5'-triphosphate
leucyl-tRNA synthetase (LeuRS)variant T252E	Escherichia coli	E. coli tRNA(Leu)	5.1	0.0033	1545	pH 7.8, 37 °C	PMID 15110746	Open record: leucyl-tRNA synthetase (LeuRS) variant T252E, E. coli tRNA(Leu)
leucyl-tRNA synthetase (LeuRS)variant T252E	Escherichia coli	L-leucine	4.9	0.019	258	pH 7.8, 37 °C	PMID 15110746	Open record: leucyl-tRNA synthetase (LeuRS) variant T252E, L-leucine
leucyl-tRNA synthetase (LeuRS)variant Y43A	Escherichia coli	L-leucine	—	—	8.2	pH 7.5, 37 °C	PMID 42573068	Open record: leucyl-tRNA synthetase (LeuRS) variant Y43A, L-leucine
leucyl-tRNA synthetase (LeuRS)variant Y43A	Escherichia coli	norvaline	1.8	17.6	0.1	pH 7.5, 37 °C	PMID 42573068	Open record: leucyl-tRNA synthetase (LeuRS) variant Y43A, norvaline
leucyl-tRNA synthetase (LeuRS)	Methanothermobacter thermautotrophicus	L-leucineBSA added	—	—	350	pH 7.5, 50 °C	PMID 15917221	Open record: leucyl-tRNA synthetase (LeuRS), L-leucine
leucyl-tRNA synthetase (LeuRS)	Methanothermobacter thermautotrophicus	L-leucineProRS and 400 nM BSA added (LeuRS-ProRS complex)	—	—	370	pH 7.5, 50 °C	PMID 15917221	Open record: leucyl-tRNA synthetase (LeuRS), L-leucine
leucyl-tRNA synthetase (LeuRS)	Methanothermobacter thermautotrophicus	tRNALeuBSA added	—	—	750	pH 7.5, 50 °C	PMID 15917221	Open record: leucyl-tRNA synthetase (LeuRS), tRNALeu
leucyl-tRNA synthetase (LeuRS)	Methanothermobacter thermautotrophicus	tRNALeuProRS and 400 nM BSA added (LeuRS-ProRS complex)	—	—	750	pH 7.5, 50 °C	PMID 15917221	Open record: leucyl-tRNA synthetase (LeuRS), tRNALeu
leucyl-tRNA synthetase (MmLeuRS)	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	3.3	0.0015	2.2 × 10³	pH 7.8, 30 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (MmLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (MmLeuRS)variant K452A	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	3.2	0.0017	1.9 × 10³	pH 7.8, 30 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (MmLeuRS) variant K452A, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (MmLeuRS)variant K452E	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	2.8	0.0019	1.5 × 10³	pH 7.8, 30 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (MmLeuRS) variant K452E, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (MmLeuRS)variant R456A	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	1.3	0.0023	600	pH 7.8, 30 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (MmLeuRS) variant R456A, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (MmLeuRS)variant R456E	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	0.85	0.0025	340	pH 7.8, 30 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (MmLeuRS) variant R456E, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (MtbLeuRS)	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	7.8	0.0011	7091	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS), Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)	Mycobacterium tuberculosis	transcribed Mtb-tRNA^Leu	2.04	0.00274	745 calc.	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS), transcribed Mtb-tRNA^Leu
leucyl-tRNA synthetase (MtbLeuRS)variant L949A	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	5.6	0.0083	674.7	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant L949A, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant L949K	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	5	0.0273	183.2	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant L949K, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant L964A	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	7.2	0.0012	6 × 10³	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant L964A, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant L964K	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	10.6	0.0174	609.2	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant L964K, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant Q915A	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	13.3	0.0019	7 × 10³	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant Q915A, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant Q915K	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	5	0.0013	3846	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant Q915K, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant R921A	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	13.6	0.0052	2615	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant R921A, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant R921K	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	10.6	0.0018	5889	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant R921K, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant V910A	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	7	0.0011	6364	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant V910A, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant V910P	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	2.4	0.0092	260.9	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant V910P, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (MtbLeuRS)variant V910W	Mycobacterium tuberculosis	Mtb-tRNA^Leu(CAG) isolated from E. coli transformants	9.2	0.0014	6571	pH 8.2, 30 °C	PMID 23268443	Open record: leucyl-tRNA synthetase (MtbLeuRS) variant V910W, Mtb-tRNA^Leu(CAG) isolated from E. coli transformants
leucyl-tRNA synthetase (PhLeuRS)	Pyrococcus horikoshii	ATP	6.8	0.578	11.8	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS), ATP
leucyl-tRNA synthetase (PhLeuRS)	Pyrococcus horikoshii	L-leucine	6	0.00592	1013	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS), L-leucine
leucyl-tRNA synthetase (PhLeuRS)	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.17	0.0065	26.2	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS), Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant D121A	Pyrococcus horikoshii	ATP	4.7	0.551	8.5	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant D121A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant D121A	Pyrococcus horikoshii	L-leucine	6	0.00721	839	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant D121A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant D121A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.18	0.0068	26.5	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant D121A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant D98A	Pyrococcus horikoshii	ATP	4.3	0.362	11.9	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant D98A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant D98A	Pyrococcus horikoshii	L-leucine	3.2	0.00592	541	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant D98A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant D98A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.2	0.0075	26.7	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant D98A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant E113A	Pyrococcus horikoshii	ATP	5.8	0.837	6.9	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant E113A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant E113A	Pyrococcus horikoshii	L-leucine	5.2	0.00618	846	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant E113A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant E113A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.22	0.0091	24.2	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant E113A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant E114A	Pyrococcus horikoshii	ATP	4.3	0.373	11.5	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant E114A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant E114A	Pyrococcus horikoshii	L-leucine	3.1	0.00623	498	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant E114A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant E114A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.16	0.0063	25.4	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant E114A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant F119A	Pyrococcus horikoshii	ATP	4	0.547	7.3	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant F119A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant F119A	Pyrococcus horikoshii	L-leucine	3.4	0.0118	286	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant F119A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant F119A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.16	0.0064	25	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant F119A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant G695P	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.01	1.9 × 10⁻⁴	53	37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (PhLeuRS) variant G695P, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant I104A	Pyrococcus horikoshii	ATP	7.4	0.796	9.3	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant I104A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant I104A	Pyrococcus horikoshii	L-leucine	5.6	0.00569	984	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant I104A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant I104A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.18	0.0053	34	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant I104A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant I115A	Pyrococcus horikoshii	ATP	13.8	2.177	6.4	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant I115A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant I115A	Pyrococcus horikoshii	L-leucine	10.7	0.00559	1.91 × 10³	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant I115A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant I115A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.19	0.0076	25	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant I115A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant K100A	Pyrococcus horikoshii	ATP	4.6	0.531	8.7	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K100A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant K100A	Pyrococcus horikoshii	L-leucine	3.9	0.00891	438	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K100A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant K100A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.22	0.0087	25.3	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K100A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y105A	Pyrococcus horikoshii	ATP	3	0.584	5.2	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K100A/Y105A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y105A	Pyrococcus horikoshii	L-leucine	3.1	0.0063	493	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K100A/Y105A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y105A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.2	0.0073	27.4	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K100A/Y105A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y109A	Pyrococcus horikoshii	ATP	4.9	0.366	13.5	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K100A/Y109A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y109A	Pyrococcus horikoshii	L-leucine	4.6	0.0057	806	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K100A/Y109A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y109A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.16	0.0053	30.2	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K100A/Y109A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant K692A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.041	4.4 × 10⁻⁴	90	37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K692A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant K696A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.017	6.5 × 10⁻⁴	30	37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K696A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant K699A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.05	6 × 10⁻⁴	80	37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K699A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant N96A	Pyrococcus horikoshii	ATP	5.1	0.37	13.8	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant N96A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant N96A	Pyrococcus horikoshii	L-leucine	4.4	0.00537	819	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant N96A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant N96A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.16	0.0055	29.1	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant N96A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant R106A	Pyrococcus horikoshii	ATP	5.4	0.296	18.2	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R106A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant R106A	Pyrococcus horikoshii	L-leucine	4.7	0.00854	550	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R106A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant R106A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.18	0.0066	27.3	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R106A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant R698A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.067	5.2 × 10⁻⁴	130	37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R698A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant R703A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.0055	1.2 × 10⁻⁴	50	37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R703A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant R97A	Pyrococcus horikoshii	ATP	5.5	0.323	17	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R97A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant R97A	Pyrococcus horikoshii	L-leucine	5.1	0.00927	550	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R97A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant R97A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.23	0.0089	25.8	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R97A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant T101A	Pyrococcus horikoshii	ATP	5	0.383	13.1	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant T101A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant T101A	Pyrococcus horikoshii	L-leucine	3.8	0.00984	386	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant T101A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant T101A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.18	0.0054	33.3	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant T101A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant T118A	Pyrococcus horikoshii	ATP	7.7	1.157	6.6	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant T118A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant T118A	Pyrococcus horikoshii	L-leucine	8.1	0.00636	1277	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant T118A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant T118A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.21	0.0081	25.9	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant T118A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant V108A	Pyrococcus horikoshii	ATP	4.2	0.351	12	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant V108A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant V108A	Pyrococcus horikoshii	L-leucine	4.1	0.00581	706	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant V108A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant V108A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.17	0.0063	27	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant V108A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant W103A	Pyrococcus horikoshii	ATP	7.4	0.472	15.7	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant W103A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant W103A	Pyrococcus horikoshii	L-leucine	5.1	0.00871	586	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant W103A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant W103A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.22	0.0083	26.5	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant W103A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant Y105A	Pyrococcus horikoshii	ATP	6.5	0.537	12.1	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant Y105A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant Y105A	Pyrococcus horikoshii	L-leucine	4.3	0.00555	775	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant Y105A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant Y105A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.17	0.0054	31.5	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant Y105A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (PhLeuRS)variant Y109A	Pyrococcus horikoshii	ATP	4.8	0.591	8.1	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant Y109A, ATP
leucyl-tRNA synthetase (PhLeuRS)variant Y109A	Pyrococcus horikoshii	L-leucine	4.7	0.00902	521	pH 7.5, 65 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant Y109A, L-leucine
leucyl-tRNA synthetase (PhLeuRS)variant Y109A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript	0.17	0.006	28.3	pH 7.5, 37 °C	PMID 20482517	Open record: leucyl-tRNA synthetase (PhLeuRS) variant Y109A, Pyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	A. aeolicus tRNALeu	0.39	3.8 × 10⁻⁴	1026	pH 7.8, 37 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), A. aeolicus tRNALeu
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	A. aeolicus tRNALeu	1.5	3 × 10⁻⁴	5 × 10³	pH 7.8, 60 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), A. aeolicus tRNALeu
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	ATP	3.3	0.36	9.2	37 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), ATP
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	ATP	0.39	0.55	0.71	pH 7.8, 37 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), ATP
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	ATP	14.5	0.38	38.2	60 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), ATP
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	ATP	1.8	0.1123	16	pH 7.8, 60 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), ATP
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	E. coli tRNA2Leu	0.084	7.6 × 10⁻⁴	111	pH 7.8, 37 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), E. coli tRNA2Leu
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	E. coli tRNA2Leu	0.4	0.0015	267	pH 7.8, 60 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), E. coli tRNA2Leu
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	L-leucine	3.5	0.0013	2692	37 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), L-leucine
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	L-leucine	0.39	0.0064	61	pH 7.8, 37 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), L-leucine
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	L-leucine	15.5	0.0016	9688	60 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), L-leucine
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	L-leucine	1.4	0.006	233	pH 7.8, 60 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), L-leucine
leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer)	Aquifex aeolicus	tRNALeu	0.36	—	—	pH 7.8, 40 °C	PMID 12196521	Open record: leucyl-tRNA synthetase (αβ-LeuRS, α/β heterodimer), tRNALeu
leucyl-tRNA synthetase, cytoplasmic (hcLeuRS)variant G763P	Homo sapiens	human cytoplasmic tRNALeu with CAG anticodon (purified from an overproducing strain)	1.1	6.1 × 10⁻⁴	1.8 × 10³	37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase, cytoplasmic (hcLeuRS) variant G763P, human cytoplasmic tRNALeu with CAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase, cytoplasmic (hcLeuRS)variant R766A	Homo sapiens	human cytoplasmic tRNALeu with CAG anticodon (purified from an overproducing strain)	0.56	0.0017	330	37 °C	PMID 25817995	Open record: leucyl-tRNA synthetase, cytoplasmic (hcLeuRS) variant R766A, human cytoplasmic tRNALeu with CAG anticodon (purified from an overproducing strain)
NmLeuRS1	Natrialba magadii	L-leucine	0.563	0.00843	66.8	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1, L-leucine
NmLeuRS1	Natrialba magadii	L-norvaline	0.364	0.955	0.381	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1, L-norvaline
NmLeuRS1	Natrialba magadii	Natrialba magadii tRNA(Leu) (CAA anticodon), in vitro T7 transcript	0.0464	0.01101	4.21	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1, Natrialba magadii tRNA(Leu) (CAA anticodon), in vitro T7 transcript
NmLeuRS1	Natrialba magadii	Natrialba magadii tRNA(Leu) (GAG anticodon), in vitro T7 transcript	0.064	0.00773	8.28	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1, Natrialba magadii tRNA(Leu) (GAG anticodon), in vitro T7 transcript
NmLeuRS1	Natrialba magadii	Pyrococcus horikoshii tRNA(Leu) (GAG anticodon), in vitro T7 transcript	0.0418	0.01251	3.34	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1, Pyrococcus horikoshii tRNA(Leu) (GAG anticodon), in vitro T7 transcript
NmLeuRS1	Natrialba magadii	α-aminobutyric acid	0.518	37.82	0.0136	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1, α-aminobutyric acid
NmLeuRS1 (leucyl-tRNA synthetase 1)	Natrialba magadii	alpha-aminobutyric acid	0.518	37.82	0.0136	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1 (leucyl-tRNA synthetase 1), alpha-aminobutyric acid
NmLeuRS1 (leucyl-tRNA synthetase 1)	Natrialba magadii	L-norvaline	0.364	0.955	0.381	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1 (leucyl-tRNA synthetase 1), L-norvaline
NmLeuRS1 (leucyl-tRNA synthetase 1)	Natrialba magadii	Natrialba magadii tRNA-Leu(CAA) in vitro transcript	0.0464	0.01101	4.21	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1 (leucyl-tRNA synthetase 1), Natrialba magadii tRNA-Leu(CAA) in vitro transcript
NmLeuRS1 (leucyl-tRNA synthetase 1)	Natrialba magadii	Natrialba magadii tRNA-Leu(GAG) in vitro transcript	0.064	0.00773	8.28	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1 (leucyl-tRNA synthetase 1), Natrialba magadii tRNA-Leu(GAG) in vitro transcript
NmLeuRS1 (leucyl-tRNA synthetase 1)	Natrialba magadii	Natrialba magadii tRNA-Leu(GAG) in vitro transcript with A47c-to-C mutation	0.0124	0.0202	0.614	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1 (leucyl-tRNA synthetase 1), Natrialba magadii tRNA-Leu(GAG) in vitro transcript with A47c-to-C mutation
NmLeuRS1 (leucyl-tRNA synthetase 1)	Natrialba magadii	Pyrococcus horikoshii tRNA-Leu(GAG) in vitro transcript	0.0418	0.01251	3.34	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS1 (leucyl-tRNA synthetase 1), Pyrococcus horikoshii tRNA-Leu(GAG) in vitro transcript
NmLeuRS2	Natrialba magadii	L-leucine	0.00961	0.891	0.0108	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS2, L-leucine
NmLeuRS2	Natrialba magadii	Natrialba magadii tRNA(Leu) (CAA anticodon), in vitro T7 transcript	4.18 × 10⁻⁴	0.01313	0.0318	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS2, Natrialba magadii tRNA(Leu) (CAA anticodon), in vitro T7 transcript
NmLeuRS2	Natrialba magadii	Natrialba magadii tRNA(Leu) (GAG anticodon), in vitro T7 transcript	6.68 × 10⁻⁴	0.00335	0.199	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS2, Natrialba magadii tRNA(Leu) (GAG anticodon), in vitro T7 transcript
NmLeuRS2	Natrialba magadii	Pyrococcus horikoshii tRNA(Leu) (GAG anticodon), in vitro T7 transcript	0.00119	0.01643	0.0724	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS2, Pyrococcus horikoshii tRNA(Leu) (GAG anticodon), in vitro T7 transcript
NmLeuRS2 (leucyl-tRNA synthetase 2)	Natrialba magadii	Natrialba magadii tRNA-Leu(CAA) in vitro transcript	4.18 × 10⁻⁴	0.01313	0.0318	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS2 (leucyl-tRNA synthetase 2), Natrialba magadii tRNA-Leu(CAA) in vitro transcript
NmLeuRS2 (leucyl-tRNA synthetase 2)	Natrialba magadii	Natrialba magadii tRNA-Leu(GAG) in vitro transcript	6.68 × 10⁻⁴	0.00335	0.199	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS2 (leucyl-tRNA synthetase 2), Natrialba magadii tRNA-Leu(GAG) in vitro transcript
NmLeuRS2 (leucyl-tRNA synthetase 2)	Natrialba magadii	Pyrococcus horikoshii tRNA-Leu(GAG) in vitro transcript	0.00119	0.01643	0.0724	pH 9, 40 °C	PMID 24500203	Open record: NmLeuRS2 (leucyl-tRNA synthetase 2), Pyrococcus horikoshii tRNA-Leu(GAG) in vitro transcript
αβ-LeuRS (leucyl-tRNA synthetase)	Aquifex aeolicus	A. aeolicus tRNALeu	0.32	2 × 10⁻⁴	1.6 × 10³ calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase), A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)	Aquifex aeolicus	L-leucine	3.9	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase), L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant A156V	Aquifex aeolicus	A. aeolicus tRNALeu	0.84	5.1 × 10⁻⁴	1.6 × 10³ calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant A156V, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant A156V	Aquifex aeolicus	L-leucine	4.1	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant A156V, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant G237D	Aquifex aeolicus	A. aeolicus tRNALeu	0.39	1.4 × 10⁻⁴	2.8 × 10³ calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant G237D, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant G237D	Aquifex aeolicus	L-leucine	4	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant G237D, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant K160N	Aquifex aeolicus	A. aeolicus tRNALeu	0.66	2.4 × 10⁻⁴	2.8 × 10³ calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant K160N, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant K160N	Aquifex aeolicus	L-leucine	3.9	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant K160N, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant K238A	Aquifex aeolicus	A. aeolicus tRNALeu	0.42	1.1 × 10⁻⁴	3.8 × 10³ calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant K238A, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant K238A	Aquifex aeolicus	L-leucine	4.2	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant K238A, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant L283F	Aquifex aeolicus	A. aeolicus tRNALeu	0.59	1.8 × 10⁻⁴	3.3 × 10³ calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant L283F, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant L283F	Aquifex aeolicus	L-leucine	4.5	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant L283F, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant M159A	Aquifex aeolicus	A. aeolicus tRNALeu	0.47	3.8 × 10⁻⁴	1.2 × 10³ calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant M159A, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant M159A	Aquifex aeolicus	L-leucine	4.1	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant M159A, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant N152A	Aquifex aeolicus	A. aeolicus tRNALeu	0.072	2.9 × 10⁻⁴	250 calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant N152A, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant N152A	Aquifex aeolicus	L-leucine	3.8	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant N152A, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant N163A	Aquifex aeolicus	A. aeolicus tRNALeu	0.39	1 × 10⁻⁴	3.9 × 10³ calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant N163A, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant N163A	Aquifex aeolicus	L-leucine	4.3	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant N163A, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant Q234H	Aquifex aeolicus	A. aeolicus tRNALeu	0.4	2.5 × 10⁻⁴	1.6 × 10³ calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant Q234H, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant Q234H	Aquifex aeolicus	L-leucine	3.7	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant Q234H, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant Q269stop	Aquifex aeolicus	A. aeolicus tRNALeu	0.003	0.0095	0.32 calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant Q269stop, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant Q269stop	Aquifex aeolicus	L-leucine	4.1	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant Q269stop, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant R94A	Aquifex aeolicus	A. aeolicus tRNALeu	0.31	5 × 10⁻⁴	620 calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant R94A, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant R94A	Aquifex aeolicus	L-leucine	4.1	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant R94A, L-leucine
αβ-LeuRS (leucyl-tRNA synthetase)variant V286stop	Aquifex aeolicus	A. aeolicus tRNALeu	0.32	0.035	9.1 calc.	pH 7.8, 37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant V286stop, A. aeolicus tRNALeu
αβ-LeuRS (leucyl-tRNA synthetase)variant V286stop	Aquifex aeolicus	L-leucine	4.1	—	—	37 °C	PMID 15208367	Open record: αβ-LeuRS (leucyl-tRNA synthetase) variant V286stop, L-leucine