CatLogenzyme kinetics catalog

Values CatLog read from this paper

The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex

PMID 16055448doi:10.1074/jbc.m413511200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (72 values) from this paper: cytosolic leucyl-tRNA synthetase (hcLeuRS), cytosolic arginyl-tRNA synthetase (hcArgRS), cytosolic arginyl-tRNA synthetase (hcArgRS) ΔNhcArgRS from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.1.1.4, EC 6.1.1.19

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cytosolic arginyl-tRNA synthetase (hcArgRS)Homo sapiensarginine0.1 mM EDTA; 10 mM MgCl2; 4 mM ATP; 4 mM arginine7.40.00391.9 × 10³pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS)Homo sapiensarginine150 mM KCl; 5 mM MgCl2; 20 µM [3H]arginine (400 cpm/pmol)0.410.0035120pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS)Homo sapiensATP0.1 mM EDTA; 10 mM MgCl2; 4 mM ATP; 4 mM arginine8.91.1837.5pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS)Homo sapiensATP150 mM KCl; 5 mM MgCl2; 20 µM [3H]arginine (400 cpm/pmol)0.280.910.31pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS)Homo sapienscalf liver tRNA-Arg0.1 mM EDTA; 10 mM MgCl2; 4 mM ATP; 4 mM arginine6.25 × 10⁻⁵1.2 × 10⁵pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS)Homo sapienscalf liver tRNA-Arg150 mM KCl; 5 mM MgCl2; 20 µM [3H]arginine (400 cpm/pmol)0.324.1 × 10⁻⁴780pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS) ΔNhcArgRSHomo sapiensarginine0.1 mM EDTA; 10 mM MgCl2; 4 mM ATP; 4 mM arginine17.60.00444 × 10³pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS) ΔNhcArgRSHomo sapiensarginine150 mM KCl; 5 mM MgCl2; 20 µM [3H]arginine (400 cpm/pmol)0.970.0048200pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS) ΔNhcArgRSHomo sapiensATP0.1 mM EDTA; 10 mM MgCl2; 4 mM ATP; 4 mM arginine20.20.89323pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS) ΔNhcArgRSHomo sapiensATP150 mM KCl; 5 mM MgCl2; 20 µM [3H]arginine (400 cpm/pmol)0.770.6711.1pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS) ΔNhcArgRSHomo sapienscalf liver tRNA-Arg0.1 mM EDTA; 10 mM MgCl2; 4 mM ATP; 4 mM arginine189.8 × 10⁻⁵1.8 × 10⁵pH 7.5, 37 °COpen
cytosolic arginyl-tRNA synthetase (hcArgRS) ΔNhcArgRSHomo sapienscalf liver tRNA-Arg150 mM KCl; 5 mM MgCl2; 20 µM [3H]arginine (400 cpm/pmol)0.813.4 × 10⁻⁴2.4 × 10³pH 7.5, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)Homo sapiensATP8 mM KF; 4 mM leucine0.791.3080.6pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)Homo sapiensATP100 µM [14C]leucine (70 cpm/pmol)0.270.7730.35pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)Homo sapienscalf liver tRNA-Leu0.280.0019147pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)Homo sapienshuman cytosolic tRNA-Leu(AAG) in vitro transcript (unmodified)0.250.001250pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)Homo sapiensleucine8 mM KF; 4 mM leucine0.820.07511pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)Homo sapiensleucine100 µM [14C]leucine (70 cpm/pmol)0.30.007540pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRSHomo sapiensATP8 mM KF; 4 mM leucine0.741.3490.55pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRSHomo sapiensATP100 µM [14C]leucine (70 cpm/pmol)0.550.7250.76pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRSHomo sapienscalf liver tRNA-Leu0.590.0014421pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRSHomo sapienshuman cytosolic tRNA-Leu(AAG) in vitro transcript (unmodified)0.59.4 × 10⁻⁴532pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRSHomo sapiensleucine8 mM KF; 4 mM leucine0.810.06413pH 7.6, 37 °COpen
cytosolic leucyl-tRNA synthetase (hcLeuRS)variant ΔChcLeuRSHomo sapiensleucine100 µM [14C]leucine (70 cpm/pmol)0.560.005897pH 7.6, 37 °COpen