CatLogenzyme kinetics catalog

Values CatLog read from this paper

The ZN domain acts as a dynamic switch coordinating multiple-step aminoacylation in bacterial leucyl-tRNA synthetase

PMID 42573068doi:10.1093/nar/gkag786Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (27 values) from this paper: leucyl-tRNA synthetase (LeuRS) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.1.1.4

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
leucyl-tRNA synthetase (LeuRS)Escherichia coliL-leucine20 mM MgCl2; 5 mM DTT; 4 mM [gamma-32P]ATP; 1 mM PPi71.20.04011.78 × 10³ calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)Escherichia coliL-leucine10 mM MgCl2; 150 mM KCl; 4 mM ATP; 1 mM DTT; 20 µM tRNALeu3.30.0214150 calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)Escherichia coliL-leucine——1.78 × 10³pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant D162AEscherichia coliL-leucine20 mM MgCl2; 5 mM DTT; 4 mM [gamma-32P]ATP; 1 mM PPi11.10.0573194 calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant D162AEscherichia coliL-leucine10 mM MgCl2; 150 mM KCl; 4 mM ATP; 1 mM DTT; 20 µM tRNALeu1.780.063228.2 calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant R178AEscherichia coliL-leucine20 mM MgCl2; 5 mM DTT; 4 mM [gamma-32P]ATP; 1 mM PPi0.240.0054544 calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant R178AEscherichia coliL-leucine10 mM MgCl2; 150 mM KCl; 4 mM ATP; 1 mM DTT; 20 µM tRNALeu0.510.05968.6 calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant W177AEscherichia coliL-leucine20 mM MgCl2; 5 mM DTT; 4 mM [gamma-32P]ATP; 1 mM PPi0.090.23550.38 calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant W177AEscherichia coliL-leucine10 mM MgCl2; 150 mM KCl; 4 mM ATP; 1 mM DTT; 20 µM tRNALeu0.00820.37850.022 calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant Y43AEscherichia coliL-leucine20 mM MgCl2; 5 mM DTT; 4 mM [gamma-32P]ATP; 1 mM PPi1.280.15618.20 calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant Y43AEscherichia coliL-leucine10 mM MgCl2; 150 mM KCl; 4 mM ATP; 1 mM DTT; 20 µM tRNALeu0.330.32151.0 calc.pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant Y43AEscherichia coliL-leucine——8.2pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant Y43AEscherichia colinorvaline1.817.60.1pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant Y43A/W177AEscherichia coliL-leucine20 mM MgCl2; 5 mM DTT; 4 mM [gamma-32P]ATP; 1 mM PPi0.05——pH 7.5, 37 °COpen
leucyl-tRNA synthetase (LeuRS)variant Y43A/W177AEscherichia coliL-leucine10 mM MgCl2; 150 mM KCl; 4 mM ATP; 1 mM DTT; 20 µM tRNALeu0.0013——pH 7.5, 37 °COpen