Values CatLog read from this paper

A unique insertion in the CP1 domain of Giardia lamblia leucyl-tRNA synthetase

PMID 19170608doi:10.1021/bi801832jOpen in Browse

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CatLog read 13 records (39 values) from this paper: leucyl-tRNA synthetase (GlLeuRS) from Giardia lamblia.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.1.1.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	adenosine 5'-triphosphate1 mM Leu; 2 mM tetrasodium [32P]pyrophosphate; 20 nM GlLeuRS	34.5	0.7504	46	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS), adenosine 5'-triphosphate
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	adenosine 5'-triphosphate10 µM tRNALeu; 20 µM [3H]Leu; cosubstrate L-leucine, tRNALeu	3.1	0.6876	4.5	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS), adenosine 5'-triphosphate
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	Aquifex aeolicus tRNALeu(GAG), purified from an overproducing strain	0.8	0.0075	106.7	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS), Aquifex aeolicus tRNALeu(GAG), purified from an overproducing strain
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	Escherichia coli tRNALeu(GAG), purified from an overproducing strain	2.6	0.0072	361.1	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS), Escherichia coli tRNALeu(GAG), purified from an overproducing strain
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)	2.7	0.0061	442.6	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS), Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	L-leucine1 mM Leu; 2 mM tetrasodium [32P]pyrophosphate; 20 nM GlLeuRS	30.5	0.0142	2148	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS), L-leucine
leucyl-tRNA synthetase (GlLeuRS)	Giardia lamblia	L-leucine10 µM tRNALeu; 20 µM [3H]Leu; cosubstrate ATP, tRNALeu	2.8	0.009	311.1	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS), L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant ΔQ281-D294 (GlLeuRS-A)	Giardia lamblia	adenosine 5'-triphosphate	1.2	0.33	3.6	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔQ281-D294 (GlLeuRS-A), adenosine 5'-triphosphate
leucyl-tRNA synthetase (GlLeuRS)variant ΔQ281-D294 (GlLeuRS-A)	Giardia lamblia	Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)	1.3	0.0101	128.7	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔQ281-D294 (GlLeuRS-A), Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)
leucyl-tRNA synthetase (GlLeuRS)variant ΔQ281-D294 (GlLeuRS-A)	Giardia lamblia	L-leucine	1.1	0.0059	186.4	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔQ281-D294 (GlLeuRS-A), L-leucine
leucyl-tRNA synthetase (GlLeuRS)variant ΔS295-L304 (GlLeuRS-B)	Giardia lamblia	adenosine 5'-triphosphate	0.9	0.4153	2.2	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔS295-L304 (GlLeuRS-B), adenosine 5'-triphosphate
leucyl-tRNA synthetase (GlLeuRS)variant ΔS295-L304 (GlLeuRS-B)	Giardia lamblia	Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)	0.6	0.0082	73.2	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔS295-L304 (GlLeuRS-B), Giardia lamblia tRNALeu(AAG) T7 transcript (unmodified)
leucyl-tRNA synthetase (GlLeuRS)variant ΔS295-L304 (GlLeuRS-B)	Giardia lamblia	L-leucine	0.6	0.0077	77.9	pH 8.2, 45 °C	Open record: leucyl-tRNA synthetase (GlLeuRS) variant ΔS295-L304 (GlLeuRS-B), L-leucine