CatLogenzyme kinetics catalog

Values CatLog read from this paper

Functional characterization of leucine-specific domain 1 from eukaryal and archaeal leucyl-tRNA synthetases

PMID 20482517doi:10.1042/bj20100235Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 102 records (306 values) from this paper: leucyl-tRNA synthetase (PhLeuRS), leucyl-tRNA synthetase (GlLeuRS) from Pyrococcus horikoshii, Giardia lamblia.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.1.1.4

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
leucyl-tRNA synthetase (GlLeuRS)variant D173AGiardia lambliaATP34.41.02533.5pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant D173AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript0.80.0059136Open
leucyl-tRNA synthetase (GlLeuRS)variant D173AGiardia lambliaL-leucine36.30.01652.2 × 10³pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant E165AGiardia lambliaATP31.31.16926.8pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant E165AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript2.80.0083337Open
leucyl-tRNA synthetase (GlLeuRS)variant E165AGiardia lambliaL-leucine29.70.01571892pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant E167AGiardia lambliaATP60.22.12928.3pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant E167AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript1.20.0049245Open
leucyl-tRNA synthetase (GlLeuRS)variant E167AGiardia lambliaL-leucine54.60.01623.37 × 10³pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K139AGiardia lambliaATP33.20.81240.9pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K139AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript1.50.0114132Open
leucyl-tRNA synthetase (GlLeuRS)variant K139AGiardia lambliaL-leucine29.90.01511.98 × 10³pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K141AGiardia lambliaATP31.50.77240.8pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K141AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript0.80.0051157Open
leucyl-tRNA synthetase (GlLeuRS)variant K141AGiardia lambliaL-leucine33.10.01322508pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K142AGiardia lambliaATP36.20.72849.7pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K142AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript0.20.003557Open
leucyl-tRNA synthetase (GlLeuRS)variant K142AGiardia lambliaL-leucine31.70.01452186pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K144AGiardia lambliaATP31.80.82238.7pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K144AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript0.20.005238Open
leucyl-tRNA synthetase (GlLeuRS)variant K144AGiardia lambliaL-leucine28.90.01531889pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K148AGiardia lambliaATP32.10.69846pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K148AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript1.90.005380Open
leucyl-tRNA synthetase (GlLeuRS)variant K148AGiardia lambliaL-leucine28.40.01242.29 × 10³pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K152AGiardia lambliaATP8.10.8349.7pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K152AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript1.30.0051255Open
leucyl-tRNA synthetase (GlLeuRS)variant K152AGiardia lambliaL-leucine6.20.00242583pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K166AGiardia lambliaATP32.30.68747pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K166AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript2.80.0081346Open
leucyl-tRNA synthetase (GlLeuRS)variant K166AGiardia lambliaL-leucine28.70.01471952pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K170AGiardia lambliaATP310.68345.4pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant K170AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript1.40.0051275Open
leucyl-tRNA synthetase (GlLeuRS)variant K170AGiardia lambliaL-leucine28.10.01411993pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant Q154AGiardia lambliaATP7.50.67511.1pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant Q154AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript0.80.0038210Open
leucyl-tRNA synthetase (GlLeuRS)variant Q154AGiardia lambliaL-leucine5.90.00351686pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant S153AGiardia lambliaATP30.10.71142.3pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant S153AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript1.10.004275Open
leucyl-tRNA synthetase (GlLeuRS)variant S153AGiardia lambliaL-leucine32.50.01592044pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant W155AGiardia lambliaATP29.80.65345.6pH 8.2, 45 °COpen
leucyl-tRNA synthetase (GlLeuRS)variant W155AGiardia lambliaGiardia lamblia tRNALeu (AAG), in vitro T7 transcript2.50.0071352Open
leucyl-tRNA synthetase (GlLeuRS)variant W155AGiardia lambliaL-leucine31.90.01192681pH 8.2, 45 °COpen
leucyl-tRNA synthetase (PhLeuRS)Pyrococcus horikoshiiATP6.80.57811.8pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)Pyrococcus horikoshiiL-leucine60.005921013pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)Pyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.170.006526.2pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant D121APyrococcus horikoshiiATP4.70.5518.5pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant D121APyrococcus horikoshiiL-leucine60.00721839pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant D121APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.180.006826.5pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant D98APyrococcus horikoshiiATP4.30.36211.9pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant D98APyrococcus horikoshiiL-leucine3.20.00592541pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant D98APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.20.007526.7pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant E113APyrococcus horikoshiiATP5.80.8376.9pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant E113APyrococcus horikoshiiL-leucine5.20.00618846pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant E113APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.220.009124.2pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant E114APyrococcus horikoshiiATP4.30.37311.5pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant E114APyrococcus horikoshiiL-leucine3.10.00623498pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant E114APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.160.006325.4pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant F119APyrococcus horikoshiiATP40.5477.3pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant F119APyrococcus horikoshiiL-leucine3.40.0118286pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant F119APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.160.006425pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant I104APyrococcus horikoshiiATP7.40.7969.3pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant I104APyrococcus horikoshiiL-leucine5.60.00569984pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant I104APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.180.005334pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant I115APyrococcus horikoshiiATP13.82.1776.4pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant I115APyrococcus horikoshiiL-leucine10.70.005591.91 × 10³pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant I115APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.190.007625pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant K100APyrococcus horikoshiiATP4.60.5318.7pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant K100APyrococcus horikoshiiL-leucine3.90.00891438pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant K100APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.220.008725.3pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y105APyrococcus horikoshiiATP30.5845.2pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y105APyrococcus horikoshiiL-leucine3.10.0063493pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y105APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.20.007327.4pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y109APyrococcus horikoshiiATP4.90.36613.5pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y109APyrococcus horikoshiiL-leucine4.60.0057806pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant K100A/Y109APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.160.005330.2pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant N96APyrococcus horikoshiiATP5.10.3713.8pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant N96APyrococcus horikoshiiL-leucine4.40.00537819pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant N96APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.160.005529.1pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant R106APyrococcus horikoshiiATP5.40.29618.2pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant R106APyrococcus horikoshiiL-leucine4.70.00854550pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant R106APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.180.006627.3pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant R97APyrococcus horikoshiiATP5.50.32317pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant R97APyrococcus horikoshiiL-leucine5.10.00927550pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant R97APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.230.008925.8pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant T101APyrococcus horikoshiiATP50.38313.1pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant T101APyrococcus horikoshiiL-leucine3.80.00984386pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant T101APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.180.005433.3pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant T118APyrococcus horikoshiiATP7.71.1576.6pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant T118APyrococcus horikoshiiL-leucine8.10.006361277pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant T118APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.210.008125.9pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant V108APyrococcus horikoshiiATP4.20.35112pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant V108APyrococcus horikoshiiL-leucine4.10.00581706pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant V108APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.170.006327pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant W103APyrococcus horikoshiiATP7.40.47215.7pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant W103APyrococcus horikoshiiL-leucine5.10.00871586pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant W103APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.220.008326.5pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant Y105APyrococcus horikoshiiATP6.50.53712.1pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant Y105APyrococcus horikoshiiL-leucine4.30.00555775pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant Y105APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.170.005431.5pH 7.5, 37 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant Y109APyrococcus horikoshiiATP4.80.5918.1pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant Y109APyrococcus horikoshiiL-leucine4.70.00902521pH 7.5, 65 °COpen
leucyl-tRNA synthetase (PhLeuRS)variant Y109APyrococcus horikoshiiPyrococcus horikoshii tRNALeu (GAG), in vitro T7 transcript0.170.00628.3pH 7.5, 37 °COpen