Values CatLog read from this paper

Modulation of Aminoacylation and Editing Properties of Leucyl-tRNA Synthetase by a Conserved Structural Module

PMID 25817995doi:10.1074/jbc.m115.639492Open in Browse

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CatLog read 43 records (129 values) from this paper: leucyl-tRNA synthetase (EcLeuRS), leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer), leucyl-tRNA synthetase (PhLeuRS) and 3 more from Escherichia coli, Mycoplasma mobile, Aquifex aeolicus and 2 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.1.1.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
chimeric leucyl-tRNA synthetase MmLeuRS-CP1/LSDchimera MmLeuRS-CP1/LSD	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	2.1	0.0028	780	pH 7.8, 30 °C	Open record: chimeric leucyl-tRNA synthetase MmLeuRS-CP1/LSD chimera MmLeuRS-CP1/LSD, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
chimeric leucyl-tRNA synthetase MmLeuRS-CP1/LSDvariant K440L/K441E	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	0.69	0.0052	130	pH 7.8, 30 °C	Open record: chimeric leucyl-tRNA synthetase MmLeuRS-CP1/LSD variant K440L/K441E, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	1.5	3 × 10⁻⁴	5 × 10³	65 °C	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer), Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R94A	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	1.3	7.5 × 10⁻⁴	1.7 × 10³	65 °C	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R94A, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R94A/R98A	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	0.67	8.1 × 10⁻⁴	830	65 °C	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R94A/R98A, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R94E	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	0.89	8.1 × 10⁻⁴	1.1 × 10³	65 °C	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R94E, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R94E/R98E	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	0.19	8.8 × 10⁻⁴	220	65 °C	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R94E/R98E, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R98A	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	1.5	3.1 × 10⁻⁴	4.8 × 10³	65 °C	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R98A, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer)variant β-R98E	Aquifex aeolicus	Aquifex aeolicus tRNALeu with GAG anticodon	1	3.9 × 10⁻⁴	2.6 × 10³	65 °C	Open record: leucyl-tRNA synthetase (AaLeuRS, alpha-beta heterodimer) variant β-R98E, Aquifex aeolicus tRNALeu with GAG anticodon
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	4.2	0.0016	2.6 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C40U mutant	3.5	0.0015	1.9 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C40U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C41U mutant	1.9	0.0014	1.3 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C41U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C41U/C42U mutant	1.3	0.0042	310	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C41U/C42U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C42U mutant	1.8	0.0027	670	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), C42U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), G12U mutant	3.9	0.0017	2.3 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), G12U mutant
leucyl-tRNA synthetase (EcLeuRS)	Escherichia coli	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), G13U mutant	4	0.0017	2.4 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript), G13U mutant
leucyl-tRNA synthetase (EcLeuRS)variant D653K	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	3.1	0.0022	1.4 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant D653K, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G662A/G665A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.7	0.0012	580	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G662A/G665A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G662P	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	2.2	0.0011	2 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G662P, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G662P/G665P	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.3	0.0014	210	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G662P/G665P, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G662R/G665R	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.8	0.0013	610	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G662R/G665R, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant G665P	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	2.1	0.0013	1.6 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant G665P, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant K671A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	4.8	0.0024	2 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant K671A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant L656K/E657K	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	1.1	6.6 × 10⁻⁴	1.7 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant L656K/E657K, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R668A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	1.4	0.0028	500	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R668A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R668A/R672A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.31	0.0022	140	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R668A/R672A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R668E	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.59	0.0054	110	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R668E, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R668E/R672E	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.18	0.0058	30	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R668E/R672E, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R672A	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	4.8	0.0024	2 × 10³	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R672A, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (EcLeuRS)variant R672E	Escherichia coli	Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)	0.53	0.0028	190	pH 7.8, 37 °C	Open record: leucyl-tRNA synthetase (EcLeuRS) variant R672E, Escherichia coli tRNALeu with GAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase (MmLeuRS)	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	3.3	0.0015	2.2 × 10³	pH 7.8, 30 °C	Open record: leucyl-tRNA synthetase (MmLeuRS), Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (MmLeuRS)variant K452A	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	3.2	0.0017	1.9 × 10³	pH 7.8, 30 °C	Open record: leucyl-tRNA synthetase (MmLeuRS) variant K452A, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (MmLeuRS)variant K452E	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	2.8	0.0019	1.5 × 10³	pH 7.8, 30 °C	Open record: leucyl-tRNA synthetase (MmLeuRS) variant K452E, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (MmLeuRS)variant R456A	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	1.3	0.0023	600	pH 7.8, 30 °C	Open record: leucyl-tRNA synthetase (MmLeuRS) variant R456A, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (MmLeuRS)variant R456E	Mycoplasma mobile	Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)	0.85	0.0025	340	pH 7.8, 30 °C	Open record: leucyl-tRNA synthetase (MmLeuRS) variant R456E, Mycoplasma mobile tRNALeu with UAA anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant G695P	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.01	1.9 × 10⁻⁴	53	37 °C	Open record: leucyl-tRNA synthetase (PhLeuRS) variant G695P, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant K692A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.041	4.4 × 10⁻⁴	90	37 °C	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K692A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant K696A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.017	6.5 × 10⁻⁴	30	37 °C	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K696A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant K699A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.05	6 × 10⁻⁴	80	37 °C	Open record: leucyl-tRNA synthetase (PhLeuRS) variant K699A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant R698A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.067	5.2 × 10⁻⁴	130	37 °C	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R698A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase (PhLeuRS)variant R703A	Pyrococcus horikoshii	Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)	0.0055	1.2 × 10⁻⁴	50	37 °C	Open record: leucyl-tRNA synthetase (PhLeuRS) variant R703A, Pyrococcus horikoshii tRNALeu with GAG anticodon (in vitro T7 transcript)
leucyl-tRNA synthetase, cytoplasmic (hcLeuRS)variant G763P	Homo sapiens	human cytoplasmic tRNALeu with CAG anticodon (purified from an overproducing strain)	1.1	6.1 × 10⁻⁴	1.8 × 10³	37 °C	Open record: leucyl-tRNA synthetase, cytoplasmic (hcLeuRS) variant G763P, human cytoplasmic tRNALeu with CAG anticodon (purified from an overproducing strain)
leucyl-tRNA synthetase, cytoplasmic (hcLeuRS)variant R766A	Homo sapiens	human cytoplasmic tRNALeu with CAG anticodon (purified from an overproducing strain)	0.56	0.0017	330	37 °C	Open record: leucyl-tRNA synthetase, cytoplasmic (hcLeuRS) variant R766A, human cytoplasmic tRNALeu with CAG anticodon (purified from an overproducing strain)